A miniaturized electrochemical platform with an integrated PDMS reservoir for label-free DNA hybridization detection using nanostructured Au electrodes

Abstract

We report the fabrication and characterization of a miniaturized electrochemical platform for the label-free detection of DNA hybridization.

Label-Free Electrochemical Biosensors for the Determination of Flaviviruses: Dengue, Zika, and Japanese Encephalitis

“…In the first case, hybridizing solution mixture is immobilized on the electrode surface and a direct electrochemical response from the hybrid duplex is recorded [66]. In the second case, a DNA probe is immobilized on the surface of the sensor, which is complementary to the target DNA in the analyzed solution, and electrochemical signal from redox/capture probes is recorded by voltammetric methods before and after hybridization [80].…”

“…The presence of metals or their oxides on the electrode surface contributes to the biocompatibility and target specificity with DNA molecules [82]. A successful example with nanostructured gold electrode (AuE) surfaces for DENV determination is the paper of Tripathy et al [80]. The authors made a miniature electrochemical sensor as a promising point-of-care platform for determining the DENV specific consensus primer at the subfemtomolar concentration level (LOD 9.7 × 10 −16 mol/L).…”

Strategies for the preparation of non-amplified and amplified genomic dengue gene samples for electrochemical DNA biosensing applications

“…However, most earlier MB research focused on synthetic DNA sequences or puried samples, and the use of MB for electrochemical detection based on large DNA sequences from real samples is currently still limited. Most recent works related to electrochemical DNA biosensors have focused on studying miniaturized electrochemical sensor devices, 22 amplication signals, 23,24 biorecognition probes with new detection mechanisms, 25 and the optimization of immobilization and hybridization conditions. 26 However, far too little attention has been focused on the preparation of genomic DNA samples for electrochemical detection.…”

Facile, Label‐Free, Non‐Enzymatic Electrochemical Nanobiosensor Platform as a Significant Step towards Continuous Glucose Monitoring

“…The main advantage is the precise control of deposition and adhesion of nanoparticles onto the surface, which in turn gives control over electrical conductivity and active surface area, thereby improving the analytical sensitivity, selectivity, and stability of the biosensors. [1,[7][8][9][10][11][12] Over the past few decades, a plethora of efforts has been put in to improve the reliability and, more so, on developing minimally invasive continuous glucose monitoring systems. The standard enzymatic-based electrochemical sensors suffer from issues related to the Glucose oxidase (Gox) enzyme stability, which makes it a non-ideal candidate for continuous glucose monitoring.…”

“…Electrochemical deposition is the most simple yet robust way of depositing these nanoparticles. The main advantage is the precise control of deposition and adhesion of nanoparticles onto the surface, which in turn gives control over electrical conductivity and active surface area, thereby improving the analytical sensitivity, selectivity, and stability of the biosensors [1,7–12] …”