A miRNA signature suggestive of nodal metastases from laryngeal carcinoma

Ricciardiello, Filippo; Capasso, Rosanna; Kawasaki, Hiromichi; Abate, Teresa; Oliva, Flavia; Lombardi, Angela; Misso, Gabriella; Ingrosso, Diego; Leone, Carlo Antonio; Iengo, Maurizio; Caraglia, Michele

doi:10.14639/0392-100x-851

Cited by 14 publications

(7 citation statements)

References 49 publications

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“…In addition, in many cholesteatoma operation cases, there is the presence of bacteria such as Pseudomonas aeruginosa and Staphylococcus aureus surrounding the cholesteatoma tissue 46 , 47 . These types of bacteria, along with many others, contain porphyrins, which also have a very prominent auto-fluorescent signature 48 .…”

Section: Discussionmentioning

confidence: 99%

Imaging guidance for cholesteatoma surgery using tissue autofluorescence

Yang

Farrell

et al. 2023

J. Biomed. Opt.

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.SignificanceCholesteatoma is an expansile destructive lesion of the middle ear and mastoid, which can result in significant complications by eroding adjacent bony structures. Currently, there is an inability to accurately distinguish cholesteatoma tissue margins from middle ear mucosa tissue, causing a high recidivism rate. Accurately differentiating cholesteatoma and mucosa will enable a more complete removal of the tissue.AimDevelop an imaging system to enhance the visibility of cholesteatoma tissue and margins during surgery.ApproachCholesteatoma and mucosa tissue samples were excised from the inner ear of patients and illuminated with 405, 450, and 520 nm narrowband lights. Measurements were made with a spectroradiometer equipped with a series of different longpass filters. Images were obtained using a red-green-blue (RGB) digital camera equipped with a long pass filter to block reflected light.ResultsCholesteatoma tissue fluoresced under 405 and 450 nm illumination. Middle ear mucosa tissue did not fluoresce under the same illumination and measurement conditions. All measurements were negligible under 520 nm illumination conditions. All spectroradiometric measurements of cholesteatoma tissue fluorescence can be predicted by a linear combination of emissions from keratin and flavin adenine dinucleotide. We built a prototype of a fluorescence imaging system using a 495 nm longpass filter in combination with an RGB camera. The system was used to capture calibrated digital camera images of cholesteatoma and mucosa tissue samples. The results confirm that cholesteatoma emits light when it is illuminated with 405 and 450 nm, whereas mucosa tissue does not.ConclusionsWe prototyped an imaging system that is capable of measuring cholesteatoma tissue autofluorescence.

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Section: Discussionmentioning

confidence: 99%

Imaging guidance for cholesteatoma surgery using tissue autofluorescence

Yang

Farrell

et al. 2023

J. Biomed. Opt.

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“…A five-gene ( EMP1 , HOXB9 , DPY19L2P1 , MMP1 and KLHDC7B ) signature was investigated to monitor the progression of LSCC ( 33 ). Various studies have also proposed prognostic model to predict survival of LSCC ( 34 , 35 ). Compared to other studies, the present IRGPM has both prognostic and immune status monitoring function.…”

Section: Discussionmentioning

confidence: 99%

Prognostic value of immune-related genes in laryngeal squamous cell carcinoma

Liu¹,

Zhang²,

Gong³

et al. 2020

Transl Cancer Res TCR

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Background: Laryngeal squamous cell carcinoma (LSCC) is a very common cancer in head and neck.A large amount of evidence indicated that immune genes take a non-negligible part in the occurrence and development of LSCC. We aimed to evaluate the prognostic value of the immune-related genes (IRGs) in LSCC.Methods: We analyzed the expression profiles of IRGs and clinical information of patients from The Cancer Genome Atlas (TCGA) dataset, including 114 LSCC and 12 non-tumor tissues. The uncover molecular mechanisms of these survival-associated IRGs (SAIRGs) were explored by the bioinformatics analyses. A novel prognostic model based on IRGs was developed through multivariate Cox regression.Results: Twenty-seven differentially expressed SAIRGs in LSCC patients. Copy number alterations, proteinprotein interaction (PPI) network, transcription factors (TFs) regulate network were used to identify molecular characteristics of these IRGs. We established an IRGs (TLR2, XCL2, CYSLTR2 and FCGR3B) based prognostic model (IRGPM), which had good performance to predict the prognosis of LSCC (AUC =0.820, P<0.05).Further analysis, we found the IRGPM can reflect the infiltration of several immune cells.Conclusions: IRGs have a potential role in the occurrence and development of LSCC, and the IRGPM can predict the overall survival rate of LSCC.

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“…Moreover, in the validation process, we underlined the diagnostic potential of miR-223, which resulted in significant upregulation in laryngeal tumor samples. A laryngeal cancer-related oncogenic function was also confirmed by Wei et al, which demonstrated the inhibitory effect of hsa_circ_0042666 on proliferation and invasion through the targeting of miR-223/TGFBR3 axis [ 9 , 10 , 11 ]. Based on these latest data, we focused on these three miRNAs to investigate their modulation also in LNECs.…”

Section: Introductionmentioning

confidence: 85%

Poorly Differentiated Neuroendocrine Larynx Carcinoma: Clinical Features and miRNAs Signature—A New Goal for Early Diagnosis and Therapy?

Ricciardiello

Falco

Tortoriello³

et al. 2021

JCM

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Laryngeal neuroendocrine carcinomas (LNECs) are rare and highly heterogeneous malignancies presenting a wide range of pathological and clinical manifestations. Herein, we retrospectively characterize ten patients diagnosticated with LNEC, five of which were defined as well-moderately differentiated neuroendocrine carcinomas, and five that were defined as poorly differentiated neuroendocrine carcinomas, according to the latest WHO classification. Clinical features were analyzed and compared between the two subgroups together with a microRNA study which evidenced a peculiar signature likely related to poorly differentiated larynx neuroendocrine carcinomas. These findings may offer new useful insights for clinicians to improve diagnosis efficiency, therapy response, and patients’ outcome for this aggressive neoplasm.

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A miRNA signature suggestive of nodal metastases from laryngeal carcinoma

Cited by 14 publications

References 49 publications

Imaging guidance for cholesteatoma surgery using tissue autofluorescence

Imaging guidance for cholesteatoma surgery using tissue autofluorescence

Prognostic value of immune-related genes in laryngeal squamous cell carcinoma

Poorly Differentiated Neuroendocrine Larynx Carcinoma: Clinical Features and miRNAs Signature—A New Goal for Early Diagnosis and Therapy?

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