A missense mutation in the transmembrane domain of CESA9 affects cell wall biosynthesis and plant growth in rice

Wang, Daofeng; Yuan, Shu; Liang, Yin; Zhao, Jinfeng; Guo, Baotai; Lan, Jinhao; Li, Xueyong

doi:10.1016/j.plantsci.2012.08.002

Cited by 29 publications

(26 citation statements)

References 27 publications

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“…RNA-seq expression profiling revealed that the three SCW genes ( TaCesA4 , 7 , 8 ) and their homoeologs were co-expressed in the mature stem tissues ( Fig 9 ). This observation provided support for these genes being functionally orthologous to the secondary wall-forming genes from other species, for example, Arabidopsis [ 10 , 44 , 45 ], barley [ 18 ], maize [ 2 , 19 ], and rice [ 46 , 47 ]. Five genes ( TaCesA1 , 2 , 3 , 5 , 6 ) and their homoeologs from the A, B and D genomes of wheat constituted a second group involved in PCW synthesis.…”

Section: Discussionmentioning

confidence: 86%

“…Structure and function of the CesA genes in wheat remains undocumented. Most studies about structural and functional characterization of CesAs have been performed in Arabidopsis [ 10 , 44 , 45 ], maize [ 2 , 19 ], and rice [ 46 , 47 ]. Bread wheat, an allohexaploid, has a complex genome, ~17 Gb in size, ~80–90% of which consists of repetitive DNA [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

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Novel Structural and Functional Motifs in cellulose synthase (CesA) Genes of Bread Wheat (Triticum aestivum, L.)

et al. 2016

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Cellulose is the primary determinant of mechanical strength in plant tissues. Late-season lodging is inversely related to the amount of cellulose in a unit length of the stem. Wheat is the most widely grown of all the crops globally, yet information on its CesA gene family is limited. We have identified 22 CesA genes from bread wheat, which include homoeologs from each of the three genomes, and named them as TaCesAXA, TaCesAXB or TaCesAXD, where X denotes the gene number and the last suffix stands for the respective genome. Sequence analyses of the CESA proteins from wheat and their orthologs from barley, maize, rice, and several dicot species (Arabidopsis, beet, cotton, poplar, potato, rose gum and soybean) revealed motifs unique to monocots (Poales) or dicots. Novel structural motifs CQIC and SVICEXWFA were identified, which distinguished the CESAs involved in the formation of primary and secondary cell wall (PCW and SCW) in all the species. We also identified several new motifs specific to monocots or dicots. The conserved motifs identified in this study possibly play functional roles specific to PCW or SCW formation. The new insights from this study advance our knowledge about the structure, function and evolution of the CesA family in plants in general and wheat in particular. This information will be useful in improving culm strength to reduce lodging or alter wall composition to improve biofuel production.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Novel Structural and Functional Motifs in cellulose synthase (CesA) Genes of Bread Wheat (Triticum aestivum, L.)

et al. 2016

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“…The cellulose content from cell wall extract of roots was 20% less in 5-day-old osmogs seedlings than in the WT (Figure 7b). A series of reports have shown that the cellulose synthase catalytic subunit genes in rice (OsCesAs) are involved in cell wall biosynthesis (Tanaka et al, 2003;Kotake et al, 2011;Wang et al, 2012). To verify whether OsCesAs expression in osmogs seedlings was altered, total RNA was isolated from roots of 5-day-old WT and osmogs seedlings.…”

Section: Osmogs Is Required For Er N-glycan Trimming and Formationmentioning

confidence: 99%

OsMOGS is required for N‐glycan formation and auxin‐mediated root development in rice (Oryza sativa L.)

Wang

et al. 2014

The Plant Journal

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SUMMARY N-glycosylation is a major modification of glycoproteins in eukaryotic cells. In Arabidopsis, great progress has been made in functional analysis of N-glycan production; however, there are few studies in monocotyledons. Here, we characterized a rice (Oryza sativa L.) osmogs mutant with shortened roots and isolated a gene coding a putative mannosyl-oligosaccharide glucosidase (OsMOGS), an ortholog of α-glucosidase I in Arabidopsis, which trims the terminal glucosyl residue of the oligosaccharide chain of nascent peptides in the endoplasmic reticulum (ER). OsMOGS is strongly expressed in rapidly cell-dividing tissues and OsMOGS protein is localized in the ER. Mutation of OsMOGS entirely blocked N-glycan maturation and inhibited high-mannose N-glycan formation. The osmogs mutant exhibited severe defects in root cell division and elongation, resulting in a short-root phenotype. In addition, osmogs plants had impaired root hair formation and elongation, and reduced root epidemic cell wall thickness due to decreased cellulose synthesis. Further analysis showed that auxin content and polar transport in osmogs roots were reduced due to incomplete N-glycosylation of the B subfamily of ATP-binding cassette transporter proteins (ABCBs). Our results demonstrate that involvement of OsMOGS in N-glycan formation is required for auxin-mediated root development in rice.

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“…Previous studies have isolated at least 11 bc mutants, including bc1, bc2, bc3, bc4, bc5, BC6, bc7, bc10, bc11, bc12, and bc15 (Li et al 2003;Yan et al 2007;Aohara et al 2009;Zhang et al 2009;Hirano et al 2010;Zhang et al 2010;Kotake et al 2011;Wu et al 2012;Rao et al 2013). Nine of these genes have been cloned and further characterization showed a division into two categories: the first category is comprised of cell wall biosynthetic genes, including OsCesA4/BC7/BC11, OsCesA7 and OsCesA9/bc6 (Tanaka et al 2003;Yan et al 2007;Zhang et al 2009;Kotake et al 2011;Wang et al 2012;Rao et al 2013), while the second category consists of regulatory factors that indirectly function in cell wall biosynthesis, including BC1, BC3, BC10, BC12, and BC15 (Li et al 2003;Hirano et al 2010;Zhang et al 2010;Wu et al 2012). Additionally, a recent report showed that the BSH1 gene, encoding an OsCYP96B4 protein, functions in the secondary cell wall formation in an unknown manner (Wang et al 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Several genes directly involved in cell wall biosynthesis have already been identified and characterized with mutants of this type. For example, the disruptions of CesA (Cellulose synthase catalytic subunit) genes, OsCesA4/BC7/BC11, OsCesA7 and OsCesA9/bc6, cause a dramatic reduction in cellulose content and the mechanical strength of plant tissues, suggesting their essential roles in synthesis of the secondary cell wall in rice (Tanaka et al 2003;Yan et al 2007;Zhang et al 2009;Kotake et al 2011;Wang et al 2012;Rao et al 2013). Additionally, regulatory mutations, indirectly involved in cell wall synthesis, also cause fragility in plant tissues.…”

Section: Introductionmentioning

confidence: 99%

BRITTLE CULM16 (BRITTLE NODE) is required for the formation of secondary cell walls in rice nodes

Wang

Ren

Chen

et al. 2017

Journal of Integrative Agriculture

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Plant cell walls constitute the skeletal structures of plant bodies, and thus confer lodging resistance for grain crops. While the basic cell wall synthesis machinery is relatively well established now, our understanding of how the process is regulated remains limited and fragmented. In this study, we report the identification and characterization of the novel rice (Oryza sativa L.) brittle culm16 (brittle node; bc16) mutant. The brittle node phenotype of the bc16 mutant appears exclusively at nodes, and resembles the previously reported bc5 mutant. Combined histochemical staining and electron microscopy assays revealed that in the bc16 mutant, the secondary cell wall formation and thickening of node sclerenchyma tissues are seriously affected after heading. Furthermore, cell wall composition assays revealed that the bc16 mutation leads to a significant reduction in cellulose and lignin contents. Using a map-based cloning approach, the bc16 locus is mapped to an approximately 1.7-Mb region of chromosome 4. Together, our findings strengthen evidence for discretely spatial differences in the secondary cell wall formation within plant bodies.

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A missense mutation in the transmembrane domain of CESA9 affects cell wall biosynthesis and plant growth in rice

Cited by 29 publications

References 27 publications

Novel Structural and Functional Motifs in cellulose synthase (CesA) Genes of Bread Wheat (Triticum aestivum, L.)

Novel Structural and Functional Motifs in cellulose synthase (CesA) Genes of Bread Wheat (Triticum aestivum, L.)

OsMOGS is required for N‐glycan formation and auxin‐mediated root development in rice (Oryza sativa L.)

BRITTLE CULM16 (BRITTLE NODE) is required for the formation of secondary cell walls in rice nodes

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