A mitochondria-localized pentatricopeptide repeat protein is required to restore hau cytoplasmic male sterility in Brassica napus

Wang, Huadong; Xiao, Qing; Wei, Chao; Chen, Hui; Chen, Xiaohan; Dai, Cheng; Wen, Jing; Ma, Chaozhi; Tu, Jinxing; Fu, Tingdong; Shen, Jinxiong

doi:10.1007/s00122-021-03777-3

Cited by 12 publications

(7 citation statements)

References 65 publications

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“…CMS is a widespread maternal genetic trait found in nearly 200 species; however, it can be restored by fertility genes in the nuclear genome ( Feng et al 2009 ; Liu et al 2016c , 2017 ; Kim and Zhang, 2018 ; Wang et al 2021 ). With the discovery and utilization of additional cytosolic types in B. napus , the efficient and rapid identification of these types are becoming increasingly important.…”

Section: Discussionmentioning

confidence: 99%

The Bnapus50K array: a quick and versatile genotyping tool for Brassica napus genomic breeding and research

Xiao

Wang

Song

et al. 2021

G3 Genes|Genomes|Genetics

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Rapeseed is a globally cultivated commercial crop, primarily grown for its oil. High-density single nucleotide polymorphism (SNP) arrays are widely used as a standard genotyping tool for rapeseed research, including for gene mapping, genome-wide association studies, germplasm resource analysis, and cluster analysis. Although considerable rapeseed genome sequencing data has been released, DNA arrays are still an attractive choice for providing additional genetic data in an era of high-throughput whole-genome sequencing. Here, we integrated re-sequencing DNA array data (32,216, and 304 SNPs) from 505 inbred rapeseed lines, allowing us to develop a sensitive and efficient genotyping DNA array, Bnapus50K, with a more consistent genetic and physical distribution of probes. A total of 42,090 high quality probes were filtered and synthesized, with an average distance between adjacent SNPs of 8 kb. To improve the practical application potential of this array in rapeseed breeding, we also added 1,618 functional probes related to important agronomic traits such as oil content, disease resistance, male sterility, and flowering time. The additional probes also included those specifically for detecting genetically modified material. These probes show a good detection efficiency and are therefore useful for gene mapping, along with crop variety improvement and identification. The novel Bnapus50K DNA array developed in this study could prove to be a quick and versatile genotyping tool for B. napus genomic breeding and research.

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Section: Discussionmentioning

confidence: 99%

The Bnapus50K array: a quick and versatile genotyping tool for Brassica napus genomic breeding and research

Xiao

Wang

Song

et al. 2021

G3 Genes|Genomes|Genetics

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“…At present, most of the restorer genes for cytoplasmic male sterility in crops are identified as PPR genes [2,40]. PPR genes can modify the CMS transcript after its transcription [9,41,42].…”

Section: Discussionmentioning

confidence: 99%

Molecular Analysis Uncovers the Mechanism of Fertility Restoration in Temperature-Sensitive Polima Cytoplasmic Male-Sterile Brassica napus

Xiao

Wang

Chen

et al. 2021

IJMS

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Temperature-sensitive male sterility is a heritable agronomic trait affected by genotype-environment interactions. In rapeseed (Brassica napus), Polima (pol) temperature-sensitive cytoplasmic male sterility (TCMS) is commonly used for two-line breeding, as the fertility of pol TCMS lines can be partially restored at certain temperatures. However, little is known about the underlying molecular mechanism that controls fertility restoration. Therefore, we aimed to investigate the fertility conversion mechanism of the pol TCMS line at two different ambient temperatures (16 °C and 25 °C). Our results showed that the anthers developed and produced vigorous pollen at 16 °C but not at 25 °C. In addition, we identified a novel co-transcript of orf224-atp6 in the mitochondria that might lead to fertility conversion of the pol TCMS line. RNA-seq analysis showed that 1637 genes were significantly differentially expressed in the fertile flowers of 596-L when compared to the sterile flower of 1318 and 596-H. Detailed analysis revealed that differentially expressed genes were involved in temperature response, ROS accumulation, anther development, and mitochondrial function. Single-molecule long-read isoform sequencing combined with RNA sequencing revealed numerous genes produce alternative splicing transcripts at high temperatures. Here, we also found that alternative oxidase, type II NAD(P)H dehydrogenases, and transcription factor Hsfs might play a crucial role in male fertility under the low-temperature condition. RNA sequencing and bulked segregant analysis coupled with whole-genome sequencing identified the candidate genes involved in the post-transcriptional modification of orf224. Overall, our study described a putative mechanism of fertility restoration in a pol TCMS line controlled by ambient temperature that might help utilise TCMS in the two-line breeding of Brassica crops.

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“…The PPR gene PPR-Nd1 in the Hui01 line (which is a homolog of BnaA09g46030D in Darmor-bzh) is a candidate Rfw gene in the finely mapped Rfw interval. BnaA09g46030D is known to contain mitochondrial localization signals and is highly upregulated in buds, indicating that it can be regulated and functions in mitochondria ( Wang H. et al, 2021 ). However, comparing the CDS sequences of PPR-Nd1 and Rfh ( BnaA09g46030D , which restores the sterile phenotype of hau CMS, Wang H. et al, 2021 ), there are multiple SNPs and one InDel (–3 bp) difference, and there are also differences in the amino acid sequences.…”

Section: Discussionmentioning

confidence: 99%

“…BnaA09g46030D is known to contain mitochondrial localization signals and is highly upregulated in buds, indicating that it can be regulated and functions in mitochondria ( Wang H. et al, 2021 ). However, comparing the CDS sequences of PPR-Nd1 and Rfh ( BnaA09g46030D , which restores the sterile phenotype of hau CMS, Wang H. et al, 2021 ), there are multiple SNPs and one InDel (–3 bp) difference, and there are also differences in the amino acid sequences. Critically, whether PPR-Nd1 can restore the fertility of WNJ01A CMS, and the degree of pollen fertility after restoration, remain unknown.…”

Section: Discussionmentioning

confidence: 99%

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Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile Line WNJ01A Originated From Brassica juncea in Brassica napus

Yang

Nong

et al. 2021

Front. Plant Sci.

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Crosses that lead to heterosis have been widely used in the rapeseed (Brassica napus L.) industry. Cytoplasmic male sterility (CMS)/restorer-of-fertility (Rf) systems represent one of the most useful tools for rapeseed production. Several CMS types and their restorer lines have been identified in rapeseed, but there are few studies on the mechanisms underlying fertility restoration. Here, we performed morphological observation, map-based cloning, and transcriptomic analysis of the F2 population developed by crossing the CMS line WNJ01A with its restorer line Hui01. Paraffin-embedded sections showed that the sporogenous cell stage was the critical pollen degeneration period, with major sporogenous cells displaying loose and irregular arrangement in sterile anthers. Most mitochondrial electron transport chain (mtETC) complex genes were upregulated in fertile compared to sterile buds. Using bulked segregant analysis (BSA)-seq to analyze mixed DNA pools from sterile and fertile F2 buds, respectively, we identified a 6.25 Mb candidate interval where Rfw is located. Using map-based cloning experiments combined with bacterial artificial chromosome (BAC) clone sequencing, the candidate interval was reduced to 99.75 kb and two pentatricopeptide repeat (PPR) genes were found among 28 predicted genes in this interval. Transcriptome sequencing showed that there were 1679 DEGs (1023 upregulated and 656 downregulated) in fertile compared to sterile F2 buds. The upregulated differentially expressed genes (DEGs) were enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) lysine degradation pathway and phenylalanine metabolism, and the downregulated DEGs were enriched in cutin, suberine, and wax biosynthesis. Furthermore, 44 DEGs were involved in pollen and anther development, such as tapetum, microspores, and pollen wall development. All of them were upregulated except a few such as POE1 genes (which encode Pollen Ole e I allergen and extensin family proteins). There were 261 specifically expressed DEGs (9 and 252 in sterile and fertile buds, respectively). Regarding the fertile bud-specific upregulated DEGs, the ubiquitin–proteasome pathway was enriched. The top four hub genes in the protein–protein interaction network (BnaA09g56400D, BnaA10g18210D, BnaA10g18220D, and BnaC09g41740D) encode RAD23d proteins, which deliver ubiquitinated substrates to the 26S proteasome. These findings provide evidence on the pathways regulated by Rfw and improve our understanding of fertility restoration.

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A mitochondria-localized pentatricopeptide repeat protein is required to restore hau cytoplasmic male sterility in Brassica napus

Cited by 12 publications

References 65 publications

The Bnapus50K array: a quick and versatile genotyping tool for Brassica napus genomic breeding and research

The Bnapus50K array: a quick and versatile genotyping tool for Brassica napus genomic breeding and research

Molecular Analysis Uncovers the Mechanism of Fertility Restoration in Temperature-Sensitive Polima Cytoplasmic Male-Sterile Brassica napus

Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile Line WNJ01A Originated From Brassica juncea in Brassica napus

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