A mitochondrial DNA polymorphism in honeybees (Apis mellifera L.)

Moritz, Robin F. A.; Hawkins, Christopher F.; Crozier, Ross H.; Mackinley, A. G.

doi:10.1007/bf01942522

Cited by 29 publications

(17 citation statements)

References 26 publications

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“…Mitochondrial markers can be used to investigate the ancestry of individual colonies or the pattern of gene flow or introgression among hybridizing populations. The first studies on European honeybee biodiversity based on mitochondrial markers date back to 1986 (Moritz et al, 1986), in which restriction fragment length polymorphism (RFLP) of the whole mitochondrial genome were used to discriminate among three European honeybee races: A. m. caucasica, A. m. ligustica and A. m. carnica. A similar approach but with different restriction enzymes was used to assess introgression between these latter two subspecies (Meixner et al, 1993) and to determine the mitochondrial variation of endemic honeybee races across their natural range .…”

Section: Box 2 Mitochondrial Dna Analysismentioning

confidence: 99%

Biodiversity, conservation and current threats to European honeybees

Rúa¹,

Jaffé²,

Dall’Olio³

et al. 2009

Apidologie

349

251

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-Europe harbours several endemic honeybee (Apis mellifera) subspecies. Yet the distribution of these subspecies is nowadays also much influenced by beekeeping activities. Large scale migratory beekeeping and trade in queens, coupled with the promiscuous mating system of honeybees, have exposed native European honeybees to increasing introgressive hybridization with managed non-native subspecies, which may lead to the loss of valuable combinations of traits shaped by natural selection. Other threats to European honeybees are factors that have caused a progressive decline in A. mellifera throughout the world in recent years, leading to large economic losses and jeopardizing ecosystem functioning. We review the biodiversity of European honeybees and summarize the management and conservation strategies employed by different countries. A comprehensive picture of the beekeeping industry in Europe is also provided. Finally we evaluate the potential threats affecting the biodiversity of European honeybee populations and provide some perspectives for future research.Apis mellifera / subspecies / biogeography / conservation / Europe / beekeeping

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Section: Box 2 Mitochondrial Dna Analysismentioning

confidence: 99%

Biodiversity, conservation and current threats to European honeybees

Rúa¹,

Jaffé²,

Dall’Olio³

et al. 2009

Apidologie

349

251

View full text Add to dashboard Cite

show abstract

“…More recently, genetic systems such as allozymes (Nunamaker and Wilson, 1982;Badino et al, 1988), nuclear DNA (Hall, 1990;Tarès et al, 1993), mitochondrial DNA (mtDNA) (Moritz et al, 1986;Smith et al, 1989Smith et al, , 1991Hunt and Page, 1992;Garnery et al, 1993;Oldroyd et al, 1995;Arias and Sheppard, 1996;Pedersen, 1996;De la Rùa et al, 2000) and microsatellites (Estoup et al, 1993;Garnery et al, 1998) have been used to study honey bee diversification. Such analysis of population genetic 336 M. Bouga et al differentiation at the molecular level contributes to better understanding of honey bee population structure by allowing the comparison of morphological, behavioural, geographical and molecular variation.…”

Section: Introductionmentioning

confidence: 99%

Genetic divergence and phylogenetic relationships of honey bee Apis mellifera (Hymenoptera: Apidae) populations from Greece and Cyprus using PCR – RFLP analysis of three mtDNA segments

et al. 2005

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-The genetic structure and phylogenetic relationships among six honey bee populations were studied using RFLP analysis on three PCR-amplified mtDNA gene segments (16s rDNA, CO I, and ND 5). The populations were sampled from various areas of Greece and Cyprus and correspond to Apis mellifera adami, A. m. macedonica, A. m. cecropia, and A. m. cypria races, based on origin (Ruttner, 1988). Seven, eight and seven restriction enzymes were found to have at least one recognition site at the 16s rDNA, CO I, and ND 5 segments, respectively. Seven different haplotypes were detected and diagnostic patterns enabled us to discriminate A. m. macedonica from the rest of the populations (races). The estimated net nucleotide sequence divergence among the populations examined was found to range from 0.00 to 1.18 with the highest value observed to be between A. m. macedonica and non-A. m. macedonica populations. The trees obtained (by UPGMA and Dollo parsimony methods) revealed that the most distant population was that of A. m. macedonica.Apis mellifera / honey bee / mtDNA / genetic variability / Greece

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“…Unfortunately, few such "diagnostic" restriction site polymorphisms are known to occur among most honey bee subspecies, although size polymorphism may be relatively common (Moritz et al, 1986;Smith 1988; Arias et al, 1990;Cornuet et al, 1991;Garnery et al, 1992). Restriction site polymorphisms have been reported within particular subspecies (Smith and Brown 1990) (Nazzi, 1992 (1978).…”

Section: Introductionmentioning

confidence: 99%

Asymmetrical distribution of a mitochondrial DNA polymorphism between 2 introgressing honey bee subspecies

1993

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Summary — Mitochondrial DNA from 62 samples of Apis mellifera carnica and A m ligustica collected from within areas of endemism and an area of known introgression was extracted and digested with the restriction enzymes Accl, Bc/l, Bg/II, EcoRI and Xbal. Digestion with Xbal revealed a polymorphism with an asymmetrical distribution between the subspecies. This polymorphism occurs primarily in Austrian and Slovenian A m carnica and may be useful for studying introgression between this race and A m ligustica.

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A mitochondrial DNA polymorphism in honeybees (Apis mellifera L.)

Cited by 29 publications

References 26 publications

Biodiversity, conservation and current threats to European honeybees

Biodiversity, conservation and current threats to European honeybees

Genetic divergence and phylogenetic relationships of honey bee Apis mellifera (Hymenoptera: Apidae) populations from Greece and Cyprus using PCR – RFLP analysis of three mtDNA segments

Asymmetrical distribution of a mitochondrial DNA polymorphism between 2 introgressing honey bee subspecies

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