In this study, antibacterial activity of Nannochloropsis oculata which were grown in proper culture condition was searched with their extracts that obtain by using different solvent (methanol, ethanol, chloroform). The antibacterial effects of algal extracts were evaluated by using disc diffusion method against L. garvieae, F. psychrophilum and Y. ruckeri. As the results of the study, it was found that the chloroform extracts of N. oculata showed strong antibacterial activities, F. psychrophilum was the high resistant strain against to antibacterial effects of the extracts, L. garvieae and Y. ruckeri were the two most susceptible bacteria strains. The results confirm the possible use of N. oculata as a source of antimicrobial compounds or as a health-promoting food for aquaculture.