A mixture containing galactooligosaccharide, produced by the enzymic activity of Bifidobacterium bifidum, reduces Salmonella enterica serovar Typhimurium infection in mice

Searle, Laura E. J.; Best, Angus; Núñez, Alejandro; Salguero, Francisco J.; Johnson, Linda; Weyer, Ute; Dugdale, Alex; Cooley, W. A.; Carter, Ben; Jones, Gareth; Tzortzis, George; Woodward, Martin J.; Ragione, Roberto M. La

doi:10.1099/jmm.0.004390-0

Cited by 90 publications

(76 citation statements)

References 28 publications

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“…boulardii may mimic the host cell receptor to which the pathogen adheres (32,39). In that sense, Searle et al (28,29) showed that nondigestible oligosaccharides (NDOs) such as galactoligosaccharides reduce Salmonella in vitro adhesion and invasion in the human colonic HT-29-19A cell line and also in the ileum gut loop model in mice. Furthermore, S. cerevisiae var.…”

Section: Discussionmentioning

confidence: 99%

β-Galactomannan and Saccharomyces cerevisiae var. boulardii Modulate the Immune Response against Salmonella enterica Serovar Typhimurium in Porcine Intestinal Epithelial and Dendritic Cells

Badía¹,

Brufau²,

Guerrero-Zamora³

et al. 2012

Clin. Vaccine Immunol.

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Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen that causes inflammation, necrosis, and diarrhea in pigs, as well as being an important source of food-borne diseases in humans. Probiotics and prebiotics are promising alternatives to antibiotics to control and prevent intestinal infections. The present work investigated a recently developed ␤-galactomannan (␤GM) prebiotic compared to the proven probiotic Saccharomyces cerevisiae var. boulardii on porcine ileum intestinal epithelial cells (IECs) of the IPI-2I line and monocyte-derived dendritic cells (DCs) cocultured in vitro with Salmonella. We observed that both S. cerevisiae var. boulardii and ␤GM inhibited the association of Salmonella with IECs in vitro. Our data indicated that ␤GM has a higher ability than S. cerevisiae var. boulardii to inhibit Salmonella-induced proinflammatory mRNA (cytokines tumor necrosis factor alpha [TNF-␣], interleukin-1␣ [IL-1␣], IL-6, and granulocyte-macrophage colony-stimulating factor [GM-CSF] and chemokines CCL2, CCL20, and CXCL8) and at protein levels (IL-6 and CXCL8). Additionally, ␤GM and S. cerevisiae var. boulardii induced some effects on DCs that were not observed on IECs: ␤GM and S. cerevisiae var. boulardii showed slight upregulation of mRNA for TNF-␣, GM-CSF, and CCR7 receptor on porcine monocyte-derived dendritic cells (DCs). Indeed, the addition of ␤GM or S. cerevisiae var. boulardii on DCs cocultured with Salmonella showed higher gene expression (mRNA) for TNF-␣, GM-CSF, and CXCL8 compared to that of the control with Salmonella. In conclusion, the addition of ␤GM inhibits Salmonella-induced proinflammatory profiles in IECs but may promote DC activation, although associated molecular mechanisms remain to be elucidated.

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Section: Discussionmentioning

confidence: 99%

β-Galactomannan and Saccharomyces cerevisiae var. boulardii Modulate the Immune Response against Salmonella enterica Serovar Typhimurium in Porcine Intestinal Epithelial and Dendritic Cells

Badía¹,

Brufau²,

Guerrero-Zamora³

et al. 2012

Clin. Vaccine Immunol.

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“…The antibiotic-containing medium was removed and the wells were washed three times with HBSS. After washing, the cells were disrupted and the bacteria were enumerated as described above (Searle et al, 2009). …”

Section: Phenotypic Tests Of Plasmid Burden By Biolog Phenotypementioning

confidence: 99%

Curing vector for IncI1 plasmids and its use to provide evidence for a metabolic burden of IncI1 CTX-M-1 plasmid pIFM3791 on Klebsiella pneumoniae

Martín

Thomas

Laing

et al. 2016

Journal of Medical Microbiology

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Using a sequence-based approach we previously identified an IncI1 CTX-M-1 plasmid, pIFM3791, on a single pig farm in the UK that was harboured by Klebsiella pneumoniae, Escherichia coli and Salmonella enterica serotype 4,5,12:i:-. To test the hypothesis that the plasmid had spread rapidly into these differing host bacteria we wished to assess whether the plasmid conferred a fitness advantage. To do this an IncI1 curing vector was constructed and used to displace the IncI1 CTX-M-1 plasmids from K. pneumoniae strain B3791 and several other unrelated IncI1-harbouring strains indicating the potential wider application of the curing vector. The IncI1 CTX-M-1 plasmid was reintroduced by conjugation into the cured K. pneumoniae strain and also a naturally IncI1 plasmid free S. enterica serotype 4,5,12:i:-, S348/11. Original, cured and complemented strains were tested for metabolic competence using Biolog technology and in competitive growth, association to mammalian cells and biofilm formation experiments. The plasmid-cured K. pneumoniae strain grew more rapidly than either the original plasmid-carrying strain or plasmidcomplemented strains in competition experiments. Additionally, the plasmid-cured strain was significantly better at respiring with L-sorbose as a carbon source and putrescine, g-amino-nbutyric acid, L-alanine and L-proline as nitrogen sources. By contrast, no differences in phenotype were found when comparing plasmid-harbouring and plasmid-free S. enterica S348/11. In conclusion, the IncI1 curing vector successfully displaced multiple IncI plasmids. The IncI1 CTX-M1 plasmid conferred a growth disadvantage upon K. pneumoniae, possibly by imposing a metabolic burden, the mechanism of which remains to be determined.

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“…However, for ConA free glucose molecules present in this material could have partially contributed to the inhibitory effect. Although mannose is the primary ligand for Con A, this lectin also has affinity for glucose, but only about a fifth of that for mannose [30].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of lectin adherence to tissue culture cells by prebiotic carbohydrates

Maldonado¹,

Fangman²,

Pinto³

et al. 2013

ABB

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Prebiotic carbohydrates, in addition to their ability to influence the colonic microbiota, are also able to inhibit attachment of pathogenic bacteria to epithelial cells. This effect is mediated via their structural similarity to the carbohydrate ligands, located on the mucosal cell surface to which bacterial lectins attach. However, the mechanism for this inhibition is not well understood. The goal of this research was to measure the effect of two prebiotic carbohydrates, galactooligosaccharide and polydextrose, on the binding kinetics of plant lectins, having known ligand specificity, to tissue culture cells. To measure adherence, competetion experiments were conducted with HEp-2 cells exposed to nine fluorescent-labeled lectins and either the cognate ligand or a prebiotic. Fluorescence microscopy and image analysis were used to quantify adherence. Lectins that were able to bind to target cells were significantly inhibited in the presence of the cognate ligands. When prebiotics were added, inhibittion of lectin binding was observed, depending on the structural similarity between the prebiotic and the cognate ligands. In general, PDX did not inhibit lectin attachment, whereas GOS significantly inhibited most lectins. This research suggests that receptor sites located on the surface of epithelial HEp-2 cells are structurally similar to GOS.

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A mixture containing galactooligosaccharide, produced by the enzymic activity of Bifidobacterium bifidum, reduces Salmonella enterica serovar Typhimurium infection in mice

Cited by 90 publications

References 28 publications

β-Galactomannan and Saccharomyces cerevisiae var. boulardii Modulate the Immune Response against Salmonella enterica Serovar Typhimurium in Porcine Intestinal Epithelial and Dendritic Cells

β-Galactomannan and Saccharomyces cerevisiae var. boulardii Modulate the Immune Response against Salmonella enterica Serovar Typhimurium in Porcine Intestinal Epithelial and Dendritic Cells

Curing vector for IncI1 plasmids and its use to provide evidence for a metabolic burden of IncI1 CTX-M-1 plasmid pIFM3791 on Klebsiella pneumoniae

Inhibition of lectin adherence to tissue culture cells by prebiotic carbohydrates

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