A mixture of Azotobacter, Azospirillum, and Klebsiella strains improves root-rot disease complex management and promotes growth in sunflowers in calcareous soil

El_Komy, Mahmoud H.; Hassouna, Mohamed G.; Abou-Taleb, E. M.; Al‐Sarar, Ali S.; Abobakr, Yasser

doi:10.1007/s10658-019-01921-w

Cited by 29 publications

(21 citation statements)

References 34 publications

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“…While Azotobacter spp. also can fix nitrogen in a non-symbiotic way, other plant growth promoting traits like phytohormone production and phosphorous solubilization are responsible of promoting plant growth (Antoun and Kloepper, 2001;El_Komy et al, 2020). Arthrobacter spp.…”

Section: Discussionmentioning

confidence: 99%

Temporal Dynamics of Rhizobacteria Found in Pequin Pepper, Soybean, and Orange Trees Growing in a Semi-arid Ecosystem

Diaz-Garza

Fierro-Rivera

Pacheco

et al. 2020

Front. Sustain. Food Syst.

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Harsh environmental conditions in drylands force plants and their associated microbial communities to adapt to abiotic stresses. In semi-arid environments, climatic conditions and poor agricultural management have a strong impact on plant yield and thus, enhancing soil fertility by means of beneficial microorganisms such as plant growth-promoting rhizobacteria (PGPR) has been proposed as part of sustainable agricultural management. As drylands will increase due to climate change, studying microbial community dynamics of crops under such conditions is crucial as it might favor rhizobacteria adapted to drought. While the microbiome of many native dryland crops has been characterized, the microbial community composition from non-native crops under semi-arid environmental conditions is understudied. Thus, the aim of this study was to characterize the bacterial community associated with the roots of three crops with different growth cycles, cultivated in the same semi-arid environment, to understand their microbial community composition during the season with the highest temperature in northeast Mexico. We performed high throughput sequencing of the V3-V4 region of the 16S rRNA gene from root samples of Pequin pepper, soybean and orange trees. Classified taxa were evaluated according to crop, sampling time and climatological parameters. Our findings revealed that changes in temporal dynamics of microbial communities correlate with environmental temperature. Moreover, the microbial community of pepper was more diverse and differed from that of soybean and orange. Regarding PGPR, 47.6% of the genera were shared among crops with a high relative abundance of Bacillus, but we also detected crop-specific microbial associations where Serratia was specific to orange trees and Rhodobacter to pepper. When analyzing PGPR in correlation to climatological parameters, Bacillus was found to thrive under lower precipitation rates, higher temperatures and higher evaporation rates in pepper and orange. In contrast, some PGPR commonly used in commercial biofertilizers such as Rhizobium and Azospirillum were affected by high temperatures. This study provides a better understanding of the rhizobacterial assemblies of economically relevant crops grown under a semi-arid environment.

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Section: Discussionmentioning

confidence: 99%

Temporal Dynamics of Rhizobacteria Found in Pequin Pepper, Soybean, and Orange Trees Growing in a Semi-arid Ecosystem

Diaz-Garza

Fierro-Rivera

Pacheco

et al. 2020

Front. Sustain. Food Syst.

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“…(Bjelić et al, 2015). El_Komy et al (2020) demonstrated that the use of a mixture of Azotobacter, Azospirillum, and Klebsiella significantly reduced the mycelial growth of certain pathogenic fungi such as Macrophomina phaseolina, Rhizoctonia solani, and Fusarium solani. Also, isolates of A. vinelandii have been characterized to have the ability to produce polysaccharides, such as alginate, at rates ranging from 4.88 to 5.26 g/L.…”

Section: Growth Promoting Traits and Other Substances Produced By Azomentioning

confidence: 99%

Nitrogen Fixing Azotobacter Species as Potential Soil Biological Enhancers for Crop Nutrition and Yield Stability

et al. 2021

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Biological nitrogen fixation (BNF) refers to a microbial mediated process based upon an enzymatic “Nitrogenase” conversion of atmospheric nitrogen (N2) into ammonium readily absorbable by roots. N2-fixing microorganisms collectively termed as “diazotrophs” are able to fix biologically N2 in association with plant roots. Specifically, the symbiotic rhizobacteria induce structural and physiological modifications of bacterial cells and plant roots into specialized structures called nodules. Other N2-fixing bacteria are free-living fixers that are highly diverse and globally widespread in cropland. They represent key natural source of nitrogen (N) in natural and agricultural ecosystems lacking symbiotic N fixation (SNF). In this review, the importance of Azotobacter species was highlighted as both important free-living N2-fixing bacteria and potential bacterial biofertilizer with proven efficacy for plant nutrition and biological soil fertility. In addition, we described Azotobacter beneficial plant promoting traits (e.g., nutrient use efficiency, protection against phytopathogens, phytohormone biosynthesis, etc.). We shed light also on the agronomic features of Azotobacter that are likely an effective component of integrated plant nutrition strategy, which contributes positively to sustainable agricultural production. We pointed out Azotobacter based-biofertilizers, which possess unique characteristics such as cyst formation conferring resistance to environmental stresses. Such beneficial traits can be explored profoundly for the utmost aim to research and develop specific formulations based on inoculant Azotobacter cysts. Furthermore, Azotobacter species still need to be wisely exploited in order to address specific agricultural challenges (e.g., nutrient deficiencies, biotic and abiotic constraints) taking into consideration several variables including their biological functions, synergies and multi-trophic interactions, and biogeography and abundance distribution.

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“…High temperature and low moisture make the disease more severe and result in heavy economic losses [58,59]. Charcoal rot negatively affects the growth and yield of soybeans [28], strawberries [29], common beans [30], sorghum [31][32][33], chickpeas [34] and sunflowers [25,34,35] globally. Similarly, the severity of the disease differs among various hosts [60].…”

Section: Discussionmentioning

confidence: 99%

“…Recently, plant growth-promoting rhizobacteria (PGPR) have been recommended for the management of soil-borne diseases. These PGPRs include Azotobacter, Azospirillum and Klebsiella, all of which are used to improve the crop productivity [24,25]. The PGPRs compete with the pathogens in dual ways, i.e., either compete for nutrients or produce antibiotics, hydrogen cyanide and siderophores, all of which suppress pathogens [19,24,26].…”

Section: Introductionmentioning

confidence: 99%

“…Khare and Arora [27] recently reported that the biosurfactant-based formulation of Pseudomonas guariconensis effectively controlled the charcoal rot of sunflowers. Nonetheless, PGPRs have been proved effective in controlling charcoal rot in soybeans [28], strawberries [29], common beans [30], sorghum [31][32][33], chickpeas [34] and sunflowers [25,34,35]. However, these PGPRs have not been combined with macronutrients and biochar for inferring their combined effects on charcoal rot suppression.…”

Section: Introductionmentioning

confidence: 99%

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Sulfur Application Combined with Planomicrobium sp. Strain MSSA-10 and Farmyard Manure Biochar Helps in the Management of Charcoal Rot Disease in Sunflower (Helianthus annuus L.)

et al. 2021

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Sunflower (Helianthus annuus L.), a member of the Asteraceae, is one of the major oilseed crops around the world. Charcoal rot caused by Macrophomina phaseolina (Tassi) Goid is the most damaging disease of sunflowers globally. Fungicides are mostly used to control charcoal rot; however, these cause environmental pollution and pose adverse effects on the ecosystem. Therefore, ecofriendly management options are inevitable for the management of charcoal rot disease. Plant mineral nutrition, the use of plant growth-promoting rhizobacteria and biochar have recently been manipulated for the management of different plant diseases. However, the interactive effects of all these treatments have rarely been tested on charcoal rot suppression in sunflowers. This study assessed the influence of sulfur (0 and 2.25 mg/kg) combined with farmyard manure biochar (2%), NPK (20:20:20 mg/kg) and three different plant growth-promoting rhizobacteria (PGPR) strains on the charcoal rot suppression growth, yield, biochemistry and physiology of sunflower. The PGPR strains included in the study were Bacillus sp. strain MR-1/2 (regarded as PGPR1), Achromobacter sp. strain FB-14 (regarded as PGPR2) and Planomicrobium sp. strain MSSA-10 (regarded as PGPR3). The charcoal rot infestation was induced by inoculating the soil with M. phaseolina, and the impacts of the different treatments were studied on the disease infestation, growth, yield, biochemistry and physiology of sunflowers under 0 and 2.25-mg/kg S application. The results revealed that farmyard manure biochar and Planomicrobium sp. strain MSSA-10 in combination with 2.25-mg/kg S proved effective for the management of charcoal rot disease through regulating the antioxidant enzymes’ activities and strengthening the immune system of sunflower plants. The studied health markers (total chlorophyll content and carotenoids) and stress markers (total protein content, catalase and peroxidase) were significantly altered by the applied treatments under 0 and 2.25-mg/kg S applications. The findings of the experiment indicated that both farmyard manure biochar and Planomicrobium sp. strain MSSA-10, combined with 2.25-mg/kg S, could be used to enhance the crop yield and manage charcoal rot disease in sunflowers. Farmyard manure biochar and Planomicrobium sp. strain MSSA-10 are an easy-to-apply, cost-effective, ecofriendly and sustainable option for the management of charcoal rot disease in sunflowers.

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A mixture of Azotobacter, Azospirillum, and Klebsiella strains improves root-rot disease complex management and promotes growth in sunflowers in calcareous soil

Cited by 29 publications

References 34 publications

Temporal Dynamics of Rhizobacteria Found in Pequin Pepper, Soybean, and Orange Trees Growing in a Semi-arid Ecosystem

Temporal Dynamics of Rhizobacteria Found in Pequin Pepper, Soybean, and Orange Trees Growing in a Semi-arid Ecosystem

Nitrogen Fixing Azotobacter Species as Potential Soil Biological Enhancers for Crop Nutrition and Yield Stability

Sulfur Application Combined with Planomicrobium sp. Strain MSSA-10 and Farmyard Manure Biochar Helps in the Management of Charcoal Rot Disease in Sunflower (Helianthus annuus L.)

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