A Mixture of <i>trans</i>, <i>trans</i> Conjugated Linoleic Acid Induces Apoptosis in MCF-7 Human Breast Cancer Cells with Reciprocal Expression of Bax and Bcl-2

Islam, Mohammad Aminul; Kim, Young S; Jang, Wook Jin; Lee, Seon M; Kim, Hoon G.; Kim, So Y; Kim, Jeong O.; Ha, Yeong Lae

doi:10.1021/jf8004977

Cited by 39 publications

(64 citation statements)

References 27 publications

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“…The MTT-based assay was used to estimate viable cell number [14]. The absorbance was measured at 570 nm using an Anthos model 2020 microplate reader (Anthos Labtech Instruments, Wals., Austria).…”

Section: Cytotoxicity Testsmentioning

confidence: 99%

Anti-proliferative Effects of β-ionone on Human Lung Cancer A-549 Cells

Lee¹,

Kim²,

Jang³

et al. 2013

Journal of Life Science

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The anti-proliferative activity of β-ionone was investigated on human non-small lung cancer A-549 cells (designated A-549 cells). A-549 cells were treated with various concentrations of β-ionone (1, 5, 10, and 15 μM) for two, four, and six days. Biochemical markers related to the growth inhibition of A-549 cells by β-ionone were measured at the second day of incubation. β-Ionone inhibited the growth of A-549 cells by dose-and time-dependent manners, resulting in an IC50 of 5.0 μg/ml at the second day of incubation. β-Ionone induced apoptosis by a dose-dependent manner. β-Ionone increased levels of p53, p21, and Bax proteins, but suppressed expression of the Bcl-2 protein. Similarly, β-ionone enhanced cytochrome c release from the mitochondria to the cytosol, and induced activation of caspase-9 and -3. Additionally, β-ion-one reduced cPLA2 and COX-2 protein levels. These results suggest that the β-ionone inhibits the proliferation of A-549 cells through reciprocal regulation of Bax and Bcl-2 gene expression and suppression of cPLA2 and COX-2 protein expressions.

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Section: Cytotoxicity Testsmentioning

confidence: 99%

Anti-proliferative Effects of β-ionone on Human Lung Cancer A-549 Cells

Lee¹,

Kim²,

Jang³

et al. 2013

Journal of Life Science

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“…Some of the observed changes consistent with the induction of apoptosis include: increased wild-type p53 protein levels, chromatin condensation, increased pro-apoptotic bax and bak protein levels, reduced anti-apoptotic bcl-2 protein levels, increased cytochrome c in the cytosol, and increased cleavage of initiator and effector caspases, reduced COX-2 activity and the generation of PGE 2 [25,51,52,[54][55][56][57]. A recent report indicated that t10,c12 CLA induces apoptosis in TM4t mammary tumour cells using certain components of the endoplasmic reticulum (ER) stress response, an alternative pathway to apoptosis in addition to the classical death receptor ("extrinsic") or mitochondrial ("intrinsic") pathways [53].…”

Section: Induction Of Apoptosismentioning

confidence: 98%

The Role of Conjugated Linoleic Acid in Breast Cancer Growth and Development

Amarù¹,

Biondo²,

Field³

2010

TONUTRAJ

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Conjugated linoleic acid (CLA) consists of a group of naturally occurring and synthetic positional and geometric (cis-trans) stereoisomers of the polyunsaturated fatty acid linoleic acid. The cis-9,trans-11 (c9,t11) CLA isomer (the most prevalent form found in ruminant-derived foods) and the trans-10,cis-12 (t10,c12) CLA isomer (present in commercial preparations) are the two most widely studied CLA isomers in breast cancer. Studies using both animal and cell culture models indicate that these CLA isomers, when added to the diet or included in the cell culture medium, inhibit mammary tumour initiation, promotion and progression in rodents, and alter tumour cell viability in vitro. The mechanism of CLA's anticancer effect is not well understood, but may involve interference with the cell cycle, induction of apoptosis, modulation of gene expression via the activation of peroxisome proliferator-activated receptors, lipid peroxidation, modulation of the tumour microenvironment, changes to the structure and/or function of the cell membrane, and interference with growth factor receptor signaling. A greater understanding of the mechanism of action of CLA will support the development of clinical trials to evaluate the potential effectiveness of CLA in the treatment of breast cancer.

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“…또한 심근경색은 섬유질이 많은 콩, 현미, 잡곡, 등푸른 생선, 딸기 등의 섭취로 예방할 수 있다 [4,28,30]. 최근 conjugated linoleic acid (CLA)는 1987년에 항암성 물질 로 개발 [11]된 이후, 항당뇨성, 항관절염성 등에 관하여 많은 연구가 수행되었다 [1,8,9,[11][12][13][14][15]21,22]. Lee (Fig.…”

Section: 서 론 심근경색(Myocardial Infarction)은 관상동맥의 폐쇄에 따 른 심근의 허혈로 심근세포의unclassified

Conjugated Linoleic Acid (CLA) Ameliorates Hydrogen Peroxide-Induced Oxidative Stress on Rat Cardiomyoblast H9c2 Cells

박재홍¹,

문연규²,

권정민³

et al. 2012

Journal of Life Science

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Conjugated linoleic acid (CLA) exhibits several beneficial biological activities including anticarcinogenesis and body-fat reduction. Now, we report that CLA ameliorated the oxidative stress in rat cardiomyoblast cells, H9c2, treated with hydrogen peroxide (H2O2). Cells were cultured in DMEM/F-12 media at 37℃ with humidified atmosphere of 5% CO2. The cells, cultured for 48 hrs, were seeded at a density 3.5×10 3 cell/well in a 24 well-plate and incubated for 24 hr. Using these cells, two experiments were performed: the cytotoxicity test of CLA (10,20,30, 40, and 50 μMs), and the oxidative stress amelioration test of CLA (20 and 50 μMs) against cells treated with H2O2 (10 and 50 μMs) for 1 and 2 hrs. CLA enhanced the growth of H9c2 cells at any concentrations of CLA and at any incubation times (up to 6 days), indicating that CLA acts as a growth stimulant. No protective effect of CLA (20 and 50 μMs) was seen in cells treated 50 μM H2O2 for 1 and 2 hr, but these CLA concentrations ameliorated (p<0.05) the adverse effect of 10 μM H2O2 in cells treated for 1 hr. These CLA concentrations significantly (p<0.05) reduced the proportion of apoptotic cells, relative to control cells. These results suggest that CLA protected H9c2 cells from the oxidative stress of H2O2 through the suppression of cell apoptosis and could be a useful compound for the prevention of cardiac diseases caused by oxidative stress.

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A Mixture of trans, trans Conjugated Linoleic Acid Induces Apoptosis in MCF-7 Human Breast Cancer Cells with Reciprocal Expression of Bax and Bcl-2

Cited by 39 publications

References 27 publications

Anti-proliferative Effects of β-ionone on Human Lung Cancer A-549 Cells

Anti-proliferative Effects of β-ionone on Human Lung Cancer A-549 Cells

The Role of Conjugated Linoleic Acid in Breast Cancer Growth and Development

Conjugated Linoleic Acid (CLA) Ameliorates Hydrogen Peroxide-Induced Oxidative Stress on Rat Cardiomyoblast H9c2 Cells

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