A Model for Studying the Biomechanical Effects of Varying Ratios of Collagen Types I and III on Cardiomyocytes

Roman, Brian B.; Kumar, Shweta; Allen, Shane C.; Delgado, Mònica; Moncayo, Sabastian; Reyes, Andres; Suggs, Laura J.; Chintalapalle, Ramana V.; Li, Chunqiang; Joddar, Binata

doi:10.1007/s13239-020-00514-7

Cited by 6 publications

(3 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our laboratory has utilized extrusion-based 3D bioprinting using bioinks that are cross-linkable via visible light or chemically-induced for cardiac cells including human induced pluripotent stem cell (iPSC) derived cardiomyocytes and other cell types to engineer cardiac tissues in-vitro [ 1 , 2 , 3 , 4 ]. Such 3D bioprinted tissue platforms are robust enough to be used as a cardiac tissue model for drug testing or other studies [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Development of an extrusion-based 3D-printing strategy for clustering of human neural progenitor cells

Bilkic

Sotelo

Anujarerat

et al. 2022

Heliyon

Self Cite

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Development of an extrusion-based 3D-printing strategy for clustering of human neural progenitor cells

Bilkic

Sotelo

Anujarerat

et al. 2022

Heliyon

Self Cite

View full text Add to dashboard Cite

“…Such behavior suggests that the formed composites did not cause cytotoxic effects on HT-22 cells over 48 h. To confirm this hypothesis, CFSE staining (CellTracet) was used and quantified via flow cytometry after 48 h. In this method, the dye diffuses into cells and binds covalently to intracellular amine groups; the fluorescent intensity decreases as the cells proliferate. 47 the control sample. On the other hand, the CFSE intensity values from assays that were in contact with PVA/SA-gMNP indicated that cells proliferated statistically less than the control (p o 0.05).…”

Section: Cytocompatibility Tests With Coatingsmentioning

confidence: 99%

Poly(vinyl alcohol)/sodium alginate/magnetite composites: magnetic force microscopy for tracking magnetic domains

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Several other studies use viscoelastic engineered biomaterials for CMs, but are unable to verify that the viscoelasticity of their material is physiologically accurate due to the lack of reliable tissue measurements. 17,27,28 Cell behavior can change substantially depending on the degree of substrate viscoelasticity 12 , motivating the need for cardiac viscoelastic measurements to aid in the development of cardiac biomaterials with physiologically relevant stress relaxation.…”

mentioning

confidence: 99%

Stress relaxation rates of myocardium from failing and non-failing hearts

Gionet-Gonzales,

Gathman,

Rosas

et al. 2024

Preprint

View full text Add to dashboard Cite

The heart is a dynamic pump whose function is influenced by its mechanical properties. The viscoelastic properties of the heart, i.e. its ability to exhibit both elastic and viscous characteristics upon deformation, influence cardiac function. Viscoelastic properties change during heart failure (HF), but, direct measurements of failing and non-failing myocardial tissue stress relaxation under constant displacement are lacking. Further, how consequences of tissue remodeling, such as fibrosis and fat accumulation, alter the stress relaxation remains unknown. To address this gap, we conducted stress relaxation tests on porcine myocardial tissue to establish baseline properties of cardiac tissue. We found porcine myocardial tissue to be fast relaxing, characterized by stress relaxation tests on both a rheometer and microindenter. We then measured human left ventricle (LV) epicardium and endocardium human tissue from non-failing, ischemic HF, and non-ischemic HF patients by microindentation. We found that the ischemic HF had slower stress relaxation than non-failing endocardium; and that slower stress relaxing tissues were correlated with increased collagen deposition and increased α-smooth muscle actin (α-SMA) stress fibers, a marker of fibrosis and cardiac fibroblast activation, respectively. In the epicardium, we found that ischemic HF had faster stress relaxation than non-ischemic HF and non-failing; and that faster stress relaxation correlated with Oil Red O staining, a marker for adipose tissue. These data show that changes in stress relaxation vary across the different layers of the heart during ischemic vs. non-ischemic HF. These findings reveal how the viscoelasticity of the heart changes, which will lead to better modeling of cardiac mechanics for in vitro and in silico HF models.

show abstract

A Model for Studying the Biomechanical Effects of Varying Ratios of Collagen Types I and III on Cardiomyocytes

Cited by 6 publications

References 53 publications

Development of an extrusion-based 3D-printing strategy for clustering of human neural progenitor cells

Development of an extrusion-based 3D-printing strategy for clustering of human neural progenitor cells

Poly(vinyl alcohol)/sodium alginate/magnetite composites: magnetic force microscopy for tracking magnetic domains

Stress relaxation rates of myocardium from failing and non-failing hearts

Contact Info

Product

Resources

About