Poly(vinyl alcohol)/sodium alginate/magnetite composites: magnetic force microscopy for tracking magnetic domains

Alavarse, Alex Carvalho; Silva, Jean Bezerra; Ulrich, Henning; Petri, Denise Freitas Siqueira

doi:10.1039/d3sm00053b

Cited by 3 publications

(3 citation statements)

References 52 publications

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“…Table 2 showed that the number of live fibroblasts significantly increased after 48 and 72 h culture onto the hydrogels containing PG, VG or PG-VG compared to the PVA alone. These results are comparable to those generated with chitosan-rich PVA hydrogels (Huang et al, 2023) or sodium alginate (Alavarse et al, 2023), to list only a few. It is important to note that this is the first study designing cannabis-product-rich hydrogels and evaluating their safety.…”

Section: Skin Fibroblasts Proliferated Once In Culture On the Thc-ric...supporting

confidence: 84%

Release of delta-9-tetrahydrocannabinol from polyvinyl alcohol hydrogels and its safe interaction with human skin fibroblasts

Cui,

Zhang,

Rodrigue

et al. 2024

Front. Drug Deliv.

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This study aimed to design a THC-rich hydrogel to deliver cannabis derivatives topically. We developed hydrogels using polyvinyl alcohol (PVA) mixed with propylene glycol (PG), vegetable glycerin (VG), or both to facilitate the dissolution of delta-9-tetrahydrocannabinol (THC). The hydrogels showed a brown color, confirming the presence of the cannabinoid. They exhibit a porous structure and better mechanical properties than PVA alone. Indeed, the hydrogel containing PG, VG, or both showed elastic deformation behaviors with lower water content. FTIR analysis demonstrated the presence of THC with two specific peaks at 1,575 and 1,619 cm−1, confirming the presence of THC in the hydrogels. Human dermal fibroblast cultures onto the surface of all hydrogels confirmed the safety of the THC-rich hydrogel as the cell adhesion was comparable to the control (no THC). Furthermore, cells adhering to the hydrogels could proliferate, showing increased cell viability at 48 and 72 h, with a higher proliferation obtained with the THC-rich PVA-PG-VG hydrogels. Such cell behavior could be due to the release of the THC in the culture medium, as demonstrated by ultra-high performance liquid chromatography (UPLC), showing the presence of THC in the culture medium, ranging from 203 to 290 μg after 24 h of incubation of the hydrogels containing PG and VG or both. In comparison, the released THC from the PVA hydrogel was higher, reaching 852 μg. It is interesting to note that the THC release at 24, 48, and 72 h was slower with the hydrogels containing PG, VG, and both, compared to PVA alone. Overall, the present study has designed safe THC-rich PVA-PG-VG hydrogels as a functional delivery system for the topical use of cannabinoids to control tissue diseases, such as inflammation.

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Section: Skin Fibroblasts Proliferated Once In Culture On the Thc-ric...supporting

confidence: 84%

Release of delta-9-tetrahydrocannabinol from polyvinyl alcohol hydrogels and its safe interaction with human skin fibroblasts

Cui,

Zhang,

Rodrigue

et al. 2024

Front. Drug Deliv.

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“…Magnetic hydrogels have demonstrated significant potential in the field of neural tissue engineering. These materials can respond to external magnetic field, which facilitates the favorable growth of cells on the hydrogel matrix. , In a recent study, we reported that magnetic coatings of SA/PVA/MNPs containing aligned MNPs were rougher, decreasing the proliferation of HT-22 neural cells . Magnetic SA/PVA hydrogels were also successfully applied for Fenton degradation of organic dyes …”

Section: Introductionmentioning

confidence: 99%

“…49,50 In a recent study, we reported that magnetic coatings of SA/PVA/MNPs containing aligned MNPs were rougher, decreasing the proliferation of HT-22 neural cells. 51 Magnetic SA/PVA hydrogels were also successfully applied for Fenton degradation of organic dyes. 52 In this study, SA/PVA compositions both in the absence and presence of MNPs at concentrations of 1.0, 2.5, and 5.0 mg/ mL were subjected to 3D printing and subsequently crosslinked using Ca 2+ ions via postspraying.…”

Section: Introductionmentioning

confidence: 99%

Magnetic Force Microscopy and Nanoindentation on 3D Printed Magnetic Scaffolds for Neuronal Cell Growth

Alavarse,

da Silva,

Ghaffari Bohlouli

et al. 2023

ACS Appl. Polym. Mater.

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This study investigated the physicochemical properties of 3D printed sodium alginate (SA)/poly(vinyl alcohol) (PVA)magnetic nanoparticle (MNP) hydrogels that were subsequently cross-linked using Ca 2+ ions via postspraying. The rheological properties of the precursor hydrogels were assessed because they play a crucial role in printability. The SA/PVA composition of 12/8 wt %, both in the absence and presence of MNPs at concentrations of 1.0 mg/mL, 2.5 mg/mL, or 5.0 mg/mL, displayed good printability. Magnetic force microscopy (MFM) evidenced the random distribution of MNPs on the hydrogel surface and the aggregation of magnetic clusters with increasing MNP content. Nanoindentation tests using a silica colloidal probe allowed estimating the elastic modulus values of swollen 3D printed scaffolds. These values ranged from 1.0 MPa (SA12/PVA8) to 7.2 MPa (SA/PVA-MNP5). Confocal microscopy confirmed the presence of cells within the interior of the 3D printed scaffolds. The cytocompatibility or cytotoxicity assays showed that all 3D printed scaffolds were cytocompatible with HT-22 cells.

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A Review of the Current State of Magnetic Force Microscopy to Unravel the Magnetic Properties of Nanomaterials Applied in Biological Systems and Future Directions for Quantum Technologies

Winkler,

Ciria,

Ahmad

et al. 2023

Nanomaterials

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Magnetism plays a pivotal role in many biological systems. However, the intensity of the magnetic forces exerted between magnetic bodies is usually low, which demands the development of ultra-sensitivity tools for proper sensing. In this framework, magnetic force microscopy (MFM) offers excellent lateral resolution and the possibility of conducting single-molecule studies like other single-probe microscopy (SPM) techniques. This comprehensive review attempts to describe the paramount importance of magnetic forces for biological applications by highlighting MFM’s main advantages but also intrinsic limitations. While the working principles are described in depth, the article also focuses on novel micro- and nanofabrication procedures for MFM tips, which enhance the magnetic response signal of tested biomaterials compared to commercial nanoprobes. This work also depicts some relevant examples where MFM can quantitatively assess the magnetic performance of nanomaterials involved in biological systems, including magnetotactic bacteria, cryptochrome flavoproteins, and magnetic nanoparticles that can interact with animal tissues. Additionally, the most promising perspectives in this field are highlighted to make the reader aware of upcoming challenges when aiming toward quantum technologies.

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Poly(vinyl alcohol)/sodium alginate/magnetite composites: magnetic force microscopy for tracking magnetic domains

Abstract: Hydrogels of poly(vinyl alcohol) (PVA)/sodium alginate (SA), and magnetic nanoparticles (MNPs) were prepared by solvent casting in the absence and in the presence of magnets, in order to obtain MNPs...

Cited by 3 publications

References 52 publications

Release of delta-9-tetrahydrocannabinol from polyvinyl alcohol hydrogels and its safe interaction with human skin fibroblasts

Release of delta-9-tetrahydrocannabinol from polyvinyl alcohol hydrogels and its safe interaction with human skin fibroblasts

Magnetic Force Microscopy and Nanoindentation on 3D Printed Magnetic Scaffolds for Neuronal Cell Growth

A Review of the Current State of Magnetic Force Microscopy to Unravel the Magnetic Properties of Nanomaterials Applied in Biological Systems and Future Directions for Quantum Technologies

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