A model of kinetics of the enzymatic hydrolysis of biopolymers – a concept for determination of hydrolysate composition

Protein enzymatic hydrolysis integrated with ultrafiltration: Thermolysin application in obtaining peptides

“…We used the model to determine the kinetic constants. In this work we have assumed as previously [1] that all reactive bonds are cut with the same rate; it means that there is only one value of the kinetic constant k.…”

“…With 49 reactive bonds occurring in the molecule of the initial substrate, 1225 different reagents may occur during hydrolysis. To describe the kinetics of the reaction we used a previously developed model [1]. The model allowed us to calculate the composition of the reaction mixture, predicting the rate of formation of the chain of the given reagent and the rate of cutting this chain to shorter chains.…”

“…the length of the respective peptides) was only attempted in the studies carried out by Fernandez and Riera [13] in which a-lactoglobulin was analysed. The results of the model simulation were compared to the results based on a simpler model developed by Trusek-Holownia and Noworyta [1]. The Trusek-Holownia and Noworyta model is universal and could be applied for multiple types of biopolymers and enzymes and types of kinetics.…”

“…Protein hydrolysis is a multisubstrate consecutive-parallel reaction, where a given protein is decomposed into a number of oligomers, which are substrates for subsequent polymerisation processes [1]. Theoretically, the products may have significant mass distribution starting from a single mer (amino acid) up to a polymer with only one amino acid cut.…”

“…According to the model applied [1], first a kinetic equation of the considered reaction catalysed by the selected enzyme should be established. Then, kinetic constants have to be experimentally determined.…”