A model to reproduce predictable full-thickness burns in an experimental 0animal

Kistler, D.; Schmidt, K.

doi:10.1016/0305-4179(88)90070-8

Cited by 18 publications

(4 citation statements)

References 4 publications

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“…A burn trauma of more than 35% BSA must be produced by a simultaneous dorsal and ventral injury and is associated with a high acute mortality in rats. 6 Many different models to inflict burn wounds in vivo in rodents are described, 1,4,6,7,10 but reports on how to fix and preserve a wound dressing in rodents are scarce. Fox and Frazier 11 present a ''saddle'' made from orthoplast.…”

Section: Discussionmentioning

confidence: 99%

Innovative scald burn model and long-term dressing protector for studies in rats

Pfurtscheller

Petnehazy²,

Goessler³

et al. 2013

Journal of Trauma and Acute Care Surgery

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R ats are frequently used for experimental studies in burns.Scalding is the easiest mechanism for provoking a dermal burn; the ability to vary water temperature, time of exposure, and the burned area make this method effective for reproducing almost any kind of thermal wound. Last but not least, rats are readily available, inexpensive, and easy to manage.In general, a scald injury in rats is inflicted on anesthetized animals by immersion of the area to be burned into a water bath. The area of exposure is determined by the aperture in the template, which can be an elaborately designed apparatus, 1 a metal wire cage, 2 or a simple metal plate with a rectangular aperture 3 through which the body part is immersed in a water bath for a specified amount of time.To establish a standard long-term method for protecting the burn wound dressings from being eaten by the rats and thereby tainting the scientific results, we searched the relevant literature and found that there was nothing available for that purpose. After several tests, the armor described later used together with our scald template was found to be satisfactory for our experiment series. With the use of the armor, the silver detected in the blood should originate only from the wound, with no additional absorption from the gut.In this article, an innovative scald protocol and armor are being presented. With this template firmly pressed on the shaved back, only the area to be burned is in contact with the boiling water. The burn wound is uniform in size and depth and can certainly be reproduced. The template is easy and fast to fabricate at a low cost and at any size. The armor ensures that the dressings stay in place for a minimum of four or even more weeks and prevents the rats from eating the silver-coated wound material. MATERIALS AND METHODS Experimental Scald ModelTen female and 4 male Sprague-Dawley rats (Himberg, Austria) weighing 250 g to 350 g were allowed to acclimatize for 1 week before use. The animals had free access to water and standard rat chow with intermittent cycles of light and dark in a temperature-controlled environment at The experiments were performed in accordance with European and Austrian laws on animal experimentation. All animals were weighed and then preanaesthetized in an anesthesia-induction box with an isoflurane-oxygen mix (0Y4% isoflurane vaporizer setting in 100% oxygen, stepwise increase; oxygen flow, 1.5 L/min.). Once asleep, midazolam (2 mg/kg), medetomidine (0.15 mg/kg) and fentanyl (5 Kg/kg) were administered subcutaneously, and the eyes were protected with oleovit-A eye ointment. The full anesthetic effect was achieved after 5 minutes, and the scalding procedure was started when there was no more toe pinch reflex. Thereafter, the back of each rat was carefully shaved with an electric razor. In the prone position, the animal was placed onto a soft cylindrical bolster, and the flexible template was closely pressed onto the rat's back. One person held the rat and the bolster, the second held the template, and the third pou...

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Section: Discussionmentioning

confidence: 99%

Innovative scald burn model and long-term dressing protector for studies in rats

Pfurtscheller

Petnehazy²,

Goessler³

et al. 2013

Journal of Trauma and Acute Care Surgery

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“…The degree of a burn injury is determined by the temperature, contact time, and pressure of the heat source. 1 Although many burn models have been previously reported, most of the models involve the manual application of hot water or hot metal to the skin. [2][3][4][5][6][7][8][9][10][11] Although considerable efforts were made to maintain constant parameters, the manual creation of such burns is difficult to control and subject to human variability, particularly when performed by different operators.…”

mentioning

confidence: 99%

Preparation of Partial-Thickness Burn Wounds in Rodents Using a New Experimental Burning Device

Sakamoto

Morimoto

Ogino

et al. 2016

Annals of Plastic Surgery

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“…Entretanto, deve-se considerar o grande porte destes animais e as dificuldades decorrentes de sua obtenção, manipulação e manutenção. Segundo Kistler et al (1988) e Aksoy et al (2002) (NORONHA et al, 2001b). Ainda, deve-se considerar que a pele de roedores é marcadamente diferente da pele humana, em termos de distribuição dos folículos pilosos, uma vez que o rato caracteriza-se por apresentar o dorso altamente piloso, o que poderia exercer um efeito protetor não observado em humanos.…”

Section: Gi(4) Gii(4) Giii(4)unclassified

Efeitos da biomembrana de látex natural (Hevea brasiliensis) em pele de ratos Wistar submetidos à lesão térmica corporal por escaldamento

Liberti¹,

Alves²,

Rici³

et al.

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Densidade de volume X Média µm micrômetro dp desvio padrão H.E Hematoxilina e eosina (coloração) GI Grupo I-Controle GI(4) Grupo I-Controle (4 dias) GI(14) Grupo I-Controle (14 dias) GII Grupo II-Queimado GII(4) Grupo II-Queimado (4 dias) GII(14) Grupo II-Queimado (14 dias) GIII Grupo III-Tratado GIII(4) Grupo III-Tratado (4 dias) GIII(14) Grupo III-Tratado (14 dias) LISTA DAS PRINCIPAIS ABREVIATURAS E SIGLAS BML Biomembrana de Látex natural LTE Lesão Térmica por Escaldamento MEV Microscopia Eletrônica de Varredura CV Coeficiente de variação V V Densidade de volume X Média µm micrômetro dp desvio padrão H.E Hematoxilina e eosina (coloração) GI Grupo I-Controle GI(4) Grupo I-Controle (4 dias) GI(14) Grupo I-Controle (14 dias) GII Grupo II-Queimado GII(4) Grupo II-Queimado (4 dias) GII(14) Grupo II-Queimado (14 dias) GIII Grupo III-Tratado GIII(4) Grupo III-Tratado (4 dias) GIII(14) Grupo III-Tratado (14 dias) LISTA DAS PRINCIPAIS ABREVIATURAS E SIGLAS BML Biomembrana de Látex natural LTE Lesão Térmica por Escaldamento MEV Microscopia Eletrônica de Varredura CV Coeficiente de variação V V Densidade de volume X Média µm micrômetro dp desvio padrão H.E Hematoxilina e eosina (coloração) GI Grupo I-Controle GI(4) Grupo I-Controle (4 dias) GI(14) Grupo I-Controle (14 dias) GII Grupo II-Queimado GII(4) Grupo II-Queimado (4 dias) GII(14) Grupo II-Queimado (14 dias) GIII Grupo III-Tratado GIII(4) Grupo III-Tratado (4 dias) GIII(14) Grupo III-Tratado (14 dias) LISTA DAS PRINCIPAIS ABREVIATURAS E SIGLAS BML Biomembrana de Látex natural LTE Lesão Térmica por Escaldamento MEV Microscopia Eletrônica de Varredura CV Coeficiente de variação V V Densidade de volume X Média µm micrômetro dp desvio padrão Aos meus pais, Euclides Bolina e Paulina Bolina; Às minhas irmãs, Daniela Bolina e Cristina Bolina; Às minhas afilhadinhas, Yasmim Bolina e Giovanna Bolina; Aos meus cunhados, Rogério Galvão e Raphael Pimentel; "Que a família comece e termine sabendo onde vai. E que o homem carregue nos ombros a graça de um pai. Que a mulher seja um céu de ternura, aconchego e calor. E que os filhos conheçam a força de onde brota o amor". Padre Zezinho Agradeço pela formação do substrato existente em minha essência, sendo os maiores responsáveis, aqui na terra, por esta conquista. Agradeço, sinceramente, a todo o investimento depositado em mim, abdicando, na maioria das vezes, de satisfazerem suas próprias vontades a fim de realizarem as minhas. Agradeço também pelo enorme incentivo e pelos abraços afetuosos nos momentos de chegada e também nos momentos de partida e até mesmo pelos abraços por telefone, pois estes também foram sentidos. Porém, peço desculpas pelas minhas ausências e pelas minhas intolerâncias. Enfim, gostaria de dizer que AMO DEMAIS TODOS VOCÊS e que todas as minhas conquistas tiveram vocês por trás, pois nada foi feito sem o incentivo e o apoio de todos! Yasmim e Giovanna, vocês são uma das minhas maiores alegrias! A Madrinha ama vocês-TANTÃO! Ao meu marido Paulo Henrique de Matos Alves "Que o amor não seja imortal, posto que é ch...

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A model to reproduce predictable full-thickness burns in an experimental 0animal

Cited by 18 publications

References 4 publications

Innovative scald burn model and long-term dressing protector for studies in rats

Innovative scald burn model and long-term dressing protector for studies in rats

Preparation of Partial-Thickness Burn Wounds in Rodents Using a New Experimental Burning Device

Efeitos da biomembrana de látex natural (Hevea brasiliensis) em pele de ratos Wistar submetidos à lesão térmica corporal por escaldamento

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