A Modification of the Technic for Determination of the Antistreptolysin Titer.

Rantz, Lowell A.; Randall, Elizabeth

doi:10.3181/00379727-59-14964

Cited by 127 publications

(35 citation statements)

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“…Os resulta-dos do ensaio são calculados automaticamente por meio de função logarítmica e apresentados em unidades internacionais -UI/ml. Os resultados obtidos pela técnica da aglutinação do látex são comparáveis aos de ensaios de neutralização com inibição de hemólise, ou seja 1UI=1.04 U Todd 13,14 . Os níveis séricos de ASO foram determinados em períodos pré-estabelecidos desde a primeira semana até 5 anos após o diagnóstico.…”

Section: Determinação Laboratorial De Asounclassified

O perfil da antiestreptolisina O no diagnóstico da febre reumática aguda

Machado¹,

Ortiz²,

Martins³

et al. 2001

J. Pediatr. (Rio J.)

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ResumoObjetivo: estabelecer o perfil dos títulos de ASO, mediante o diagnóstico diferencial da FRA com outras afecções que também cursam com níveis elevados de ASO.Métodos: foram estudados 78 casos de FRA na apresentação e seguimento, 22 de coréia isolada, 45 de infecções orofaringeanas recorrentes (IOR) e 23 de artrites idiopáticas juvenis (AIJ), com início ou reativação recente. A determinação seqüencial de ASO (UI/ml) foi realizada por ensaio nefelométrico automatizado (Behring ® -Germany) nos períodos de 0-7 dias, 1-2 semanas, 2-4 semanas, 1-2 meses, 2-4 meses, 4-6 meses, 6-12 meses, 1-2 anos, 2-3 anos, 3-4 anos e 4-5 anos após o diagnóstico.Resultados: os títulos de ASO na fase aguda da FRA apresentaram elevação significante até o intervalo de 2-4 meses (p<0,0001), quando atingiram os níveis basais e permaneceram estáveis por 5 anos na vigência de profilaxia secundária com penicilina. Os valores de ASO na apresentação da FRA também foram mais elevados e com diferença significante quando comparados aos de coréia isolada, IOR e AIJ (p=0,0025). As variações etárias e o limite superior da normalidade (320 UI/ml) foram considerados para a comparação entre estes grupos, assim como para o cálculo da sensibilidade (73,3%) e da especificidade (57,6%) mediante o diagnóstico clínico de FRA. A especificidade e valor preditivo positivo do teste aumentaram com títulos crescentes, sendo mais alta com títulos >960 UI/ml.Conclusão: esta reavaliação do perfil da ASO indicou uma resposta exuberante na fase aguda da febre reumática indicou ainda que os seus níveis séricos podem diferenciá-la de outras afecções que também cursam com níveis elevados de ASO, como as infecções orofaringeanas recorrentes ou as artrites idiopáticas juvenis em atividade.J Pediatr (Rio J) 2001; 77 (2):105-11: artrite, febre reumática, antiestreptolisina, coréia. AbstractObjective: to determine ASO titer profile by establishing ARF differential diagnoses of other diseases with high levels of ASO antibodies.Methods: we investigated 78 patients with ARF at onset and follow-up, 22 with isolated chorea at onset, 45 with recurrent oropharyngeal tonsillitis, and 23 with recent flare of juvenile idiopathic arthritis. We tested ASO with automated particle-enhanced immunonephelometric assay (Behring ® -Germany). The ASO (IU/ ml) titers were assessed at the following time intervals: 0-7 days, 1-2 weeks, 2-4 weeks, 1-2 months, 2-4 months, 4-6 months, 6-12 months, 1-2 years, 2-3 years, 3-4 years, and 4-5 years after onset of ARF.Results: ASO titers in patients diagnosed with ARF had a significant increase up to the 2-4-month time interval (P<0.0001). Baseline levels were observed afterwards in patients under regular penicillin prophylaxis. The levels of ASO in ARF were also significantly higher than in patients with isolated chorea, recurrent oropharyngeal infections or juvenile idiopathic arthritis (P=0.0025), when age-matched samples of these groups were compared. The test's sensitivity was 73.3% and the specificity was 57.6%, and it was calculated taking into a...

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Section: Determinação Laboratorial De Asounclassified

O perfil da antiestreptolisina O no diagnóstico da febre reumática aguda

Machado¹,

Ortiz²,

Martins³

et al. 2001

J. Pediatr. (Rio J.)

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“…Antiestreptolisina o (ASO), se deterrnino mediante la tecnica de dilucion en tubo de Rantz y Randall 12 . Estreptozima (STZ), se determine por aglutinacion en lamina con una suspension de globulos rojos sensibilizados con productos extracelularcs obtenidos de cultivos de cstreptococo beta hemoh'tico del grupo A. Fste examen incluyc la determinacion simultanea de los siguientes anticuerpos: antiestreptolisina O (ASO) antibialuronidasa (AH), antiestreptokinasa (AK), antiDNasa (ABD) y probablemente otros mas 13 .…”

Section: Materials Y Metodounclassified

La glomerulonefritis aguda en el niño: Estudio prospectivo de aspectos clinicos, histopatologicos e inmunologicos

U¹,

L²,

G³

et al. 1987

Rev. chil. pediatr.

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Acute glomerular nephritis in childrenForty live ehildren (23 boys) 22to 15 years old (mean age 7.7 years) with clinical and histological evidence of acute glomerular nephritis (AGN) were followed by 30 months. A.II patients were studied with percutaneous renal biopsies in tlic first month after the begining of the disease (mean 20 days) and in 31 patients, a second renal biopsy was done: in 12 at x 194 days (range 171 to" 212 days) and in 19 at x 590 days (range 418 to 999 days). With only 5 exceptions (11.1%) all patients recalled clinical evidence of antecedent cutaneous (37.8%), respiratory (51.1%) or combined cutaneous and respiratory infections (11.1%) but group A bctha hcmolitycus estrep toco ecus (GABHE) was only isolated from six patients, probably because of previous antibiotic treatment. Edema and hypertension dissapearcd in a mean of 8.8 and 4.3 days respectively. Haematuria was present in only 44%of the patients in the first urine examination, protemuria in 51% and eilindruria in 29.9% of the cases, but at last, hematuria was demonstrated in 41 /45 patients; proteinuria, that was present in a!3 cases without hematuria, finally ocurred in 95% of all the affected children and eilindruria was detected in 64%of the total series. A good correlation was found in the histologic study between the magnitude of the lesions at optic and electronic microscopy. In the first biopsy all patients showed segmental intramembranous, inrnune deposits. In the second series of renal biopsies the intensity of the lesions was, clearly, lesser at six months and the microscopical findings were already normal in three cases at 590 days.

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“…These were done by a microtitration modification of the standard method of Rantz and Randall (1945) in disposable plastic trays; 50% haemolytic end-points were read visually with the aid of a magnifying viewer.…”

Section: Methodsmentioning

confidence: 99%

Specificity of Antibodies for T Sites and F Sites of Streptolysin O

Watson

Kerr

1985

Journal of Medical Microbiology

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SUMMARY. Antistreptolysin antibody (ASO) inhibits haemolysis of erythrocytes coated with streptolysin 0 (SLO), but antistreptolysin activity may also be due to the presence in sera of peptide fragments of altered p lipoproteins. SLO-mediated haemolysis results from activity of a molecular site (the t site) distinct from that at which the SLO becomes attached to the cell (the f site), and both sites might be expected to function antigenically. Absorption studies with SLO-coated latex particles, and with SLO-coated erythrocytes in the cold, provide evidence for the existence of both classes of antibody. Results also suggest explanations for the frequent observation of false-negative and false-positive latex tests.

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A Modification of the Technic for Determination of the Antistreptolysin Titer.

Cited by 127 publications

References 0 publications

O perfil da antiestreptolisina O no diagnóstico da febre reumática aguda

O perfil da antiestreptolisina O no diagnóstico da febre reumática aguda

La glomerulonefritis aguda en el niño: Estudio prospectivo de aspectos clinicos, histopatologicos e inmunologicos

Specificity of Antibodies for T Sites and F Sites of Streptolysin O

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