Specificity of Antibodies for T Sites and F Sites of Streptolysin O

Watson, K. C.; Kerr, E. J. C.

doi:10.1099/00222615-19-1-1

Cited by 3 publications

(2 citation statements)

References 7 publications

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“…A speculative explanation of this finding may be advanced: the molecule of SLO (mol. wt 60,000) possesses, at least, two different active sites (f, t ) , both of them being antigenic (48,49). SLO binds to the biological membranes via the f site while the t site of the SLO molecule initiates the haemolytic event.…”

Section: Discussionmentioning

confidence: 99%

Effects of Streptolysin o, Picibanil (OK 432) and Interferon α2A on Cytochrome P-450-Dependent Monooxygenases and Arylamine N-Acetyltransferase in Rat Liver

Hadašová

Siegmund

Walter

et al. 1995

Immunopharmacology and Immunotoxicology

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Streptolysin O, a thiol-activated exotoxin from group A beta-haemolytic streptococci, caused a dose-dependent depression of aniline hydroxylase, aminopyrine N-demethylase and ethylmorphine N-demethylase activities when added into the hepatic microsomal mixtures from male rats at concentrations 0.02-0.4 HU/mL in vitro. The activities of 7-ethoxycoumarin O-deethylase, 7-ethylresorufin O-deethylase and 7-pentylresorufin O-depentylase were not altered with the used concentrations of the toxin. Specific antibody against haemolytic action of streptolysin O added to incubation mixtures in vitro was not able to protect streptolysin-sensitive monooxygenases from the inhibition. The addition of streptolysin O (0.01-0.8 HU/mL) into the cytosol-containing medium did not significantly influence the activity of procainamide N-acetyltransferase. Immunomodulators picibanil (OK 432) and human recombinant interferon alpha 2A which are known to suppress oxidative metabolism in vivo in humans and animals, were without effect either on the cytochrome P-450-dependent monooxygenases or on the N-acetyltransferase activity when administered in vitro at the doses real in their clinical application (0.001-0.1 KE/mL of picibanil and 10-500 U/mL of alpha-interferon).

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Section: Discussionmentioning

confidence: 99%

Effects of Streptolysin o, Picibanil (OK 432) and Interferon α2A on Cytochrome P-450-Dependent Monooxygenases and Arylamine N-Acetyltransferase in Rat Liver

Hadašová

Siegmund

Walter

et al. 1995

Immunopharmacology and Immunotoxicology

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show abstract

“…However, the rabbit antisera to SLO appeared to be directed mainly against different epitopes because, although their precipitating capacities were about the same, their neutralizing titers were quite different. Results such as these may be the reason for variations in anti-SLO titers obtained by different methods, including nephelometric, enzyme-linked immunosorbent assay, or hemolytic-activityneutralizing tests (37).…”

Section: Fractionsmentioning

confidence: 99%