A Modification of the Wu and Hoak Method for the Determination of Platelet Aggregates and Platelet Adhesion

Bowry, Sudhir K; Prentice, C. R. M.; Courtney, J. M.

doi:10.1055/s-0038-1661319

Cited by 21 publications

(7 citation statements)

References 15 publications

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“…(1) biochemistry; (2) full blood count; (3) platelets aggregation index according to Bowry's modification of Wu and Hoak's method [15]; (4) platelets aggregation caused by collagen and adenosine according to turbidimetric method [16]; (5) secretion of adenine nucleotides from platelets by fluorimetry; (6) concentration of energy-rich adenine compounds (adenosine-tri-phosphate, adenosine di-phosphate, adenosine mono-phosphate) by fluorimetry, and (7) levels of cyclic adenosine-mono-phosphate (cAMP) and cyclic guanosin mono-phosphate (cGMP) by immunoassays. After the blood sample was taken, participation of a patient in the study was completed.…”

Section: Methodsmentioning

confidence: 99%

Changes of platelets’ function in preeclampsia

2011

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AbstractIncreased aggregation of platelets during preeclampsia was shown in several studies, yet several others reported no change. The aim of our study was to investigate platelet aggregation in a group of patients suffering from preeclampsia. In a cross-sectional study blood samples were taken from 89 hospitalized patients in the third trimester of pregnancy: 38 were suffering from mild to moderate preeclampsia and 51 patients were without preeclampsia. From the blood samples platelet aggregation, secretion of adenine nucleotides from platelets, concentration of energy-rich adenine compounds and levels of cyclic adenosine-mono-phosphate and cyclic guanosine mono-phosphate in platelets were measured. In the patients with preeclampsia, the adenosine diphosphate threshold for biphasic aggregation [odds ratio (OR):.75; 95% Confidence Interval (CI): 0.55–1.02; p<0.05], total adenine nucleotides concentration in the metabolic pool of platelets (OR:0.99; CI: 0.62–1.57; p<0.01) and cyclic adenosine-mono-phosphate (OR:0.81; CI: 0.57, 1.14; p<0.05) and cyclic guanosine mono-phosphate (OR:.78; CI: 0.55–1.09; p<0.05) levels in platelets were decreased in comparison with the control group, while adenylate energy charge in the metabolic pool of platelets (OR: >100.00; CI: 0.00->100.00; p<0.05) and secretion of adenosine triphosphate (OR:.13; CI: 0.00–14.26; p<0.05) and adenosine diphosphate (OR:.77; CI: 0.08–36.79; p<0.05) were increased. The results of our study show increased activation and aggregation of platelets in pregnant females with preeclampsia.

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Section: Methodsmentioning

confidence: 99%

Changes of platelets’ function in preeclampsia

2011

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“…The difficulty of these methods is to prevent artefactual ex vivo platelet activation by careful handling and blood processing. One of the earliest measurements for detecting activation was the detection of platelet aggregates within blood as described by Wu & Hoak (1974) and then modified by Bowry et al (1985). Other methods involve measuring threshold aggregation responses to ADP/arachidonate or testing for the ability of patient's platelets to spontaneously aggregate.…”

Section: Advances In the Measurement Of Platelet Activationmentioning

confidence: 99%

Review

Harrison

2000

Br J Haematol

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“…5 Wu and Hoak collected a small amount of blood into syringes preloaded with formaldehyde and EDTA in phosphate-buffere d saline. They called this derived index of aggregation the platelet aggregate ratio.…”

Section: Introductionmentioning

confidence: 99%

Quantitative detection of platelet aggregates in whole blood without fixation

et al. 2000

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Platelet counting detects lesser degrees of platelet aggregation than conventional aggregometry. In order to prevent progressive platelet aggregation or disaggregation after sampling it is customary to fix blood samples. However fixation may introduce other artefacts. We first compared stability of platelet counts in EDTA-, citrate- and r-hirudin-anticoagulated blood from healthy volunteers. Second, the stability of platelet counts in unfixed EDTA- and hirudin-anticoagulated blood was compared with glutaraldehyde-fixed blood in the same anticoagulants. Third, the effect of in vivo heparin administration on platelet counts in EDTA- and hirudin-anticoagulated blood was studied. Platelet counts within 2 h of collection were significantly higher in EDTA- than in hirudin- or citrate-anticoagulated blood (P = 0.002 vs. hirudin and P = 0.001 vs. citrate). Twenty-four hour counts in hirudin and EDTA were unchanged (P = 0.3 and P = 0.2, respectively, vs. earlier counts). Counts in citrate increased significantly (P = 0.007; n = 10). Platelet counts in fixed blood did not differ significantly from those in unfixed blood. Heparin administered for cardiopulmonary bypass reduced platelet counts in hirudin-anticoagulated blood from (mean +/- 1 standard deviation) 180 +/- 45 to 162 +/- 30 x 10(9) l-1 (P = 0.01; n = 14), without significantly lowering counts with EDTA-anticoagulation, consistent with increased platelet aggregation. Hirudin and EDTA provided stable platelet counts, suggesting that fixation is unnecessary.

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A Modification of the Wu and Hoak Method for the Determination of Platelet Aggregates and Platelet Adhesion

Cited by 21 publications

References 15 publications

Changes of platelets’ function in preeclampsia

Changes of platelets’ function in preeclampsia

Review

Quantitative detection of platelet aggregates in whole blood without fixation

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