A modification to an established technique for the assessment of fissure caries in the rat

Green, R.M.; Hartles, R.L.

doi:10.1016/0003-9969(67)90228-2

Cited by 24 publications

(5 citation statements)

References 4 publications

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“…Only fissure caries was found in these experi ments and it was assessed using the technique de scribed by König et al [1958] but using the modi fication described by Green and Hartles [1967]. The caries was categorised as total lesions (T + B -C), advanced lesions (B + C) and very advanced lesions (C).…”

Section: Methodsmentioning

confidence: 99%

Effect of Xylitol-Supplemented Diets on the Progression and Regression of Fissure Caries in the Albino Rat

Green¹

1980

Caries Res

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Dentinal molar fissure caries in Liverpool albino rats, produced by initial exposure to dietary sucrose, could be significantly reversed by subsequent exposure to a xylitol-supplemented starch diet (X-S St). An unsupplemented starch diet had no significant effect on caries under the same conditions. Alternate feeding of sucrose and X-S St produced a sequential progression and regression of caries, respectively. Continued exposure to X-S St produced a successive regression in caries. The regression in caries can be explained in terms of the increased rate of salivary flow and the attendent rise in pH and calcium concentration that occur when xylitol is introduced into the mouth. Both of these changes should encourage re-mineralisation of a reversible lesion below a temporarily non-acidogenic plaque. Under these conditions xylitol is not merely a bland, non-cariogenic agent but is genuinely exerting a therapeutic action against caries.

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Section: Methodsmentioning

confidence: 99%

Effect of Xylitol-Supplemented Diets on the Progression and Regression of Fissure Caries in the Albino Rat

Green¹

1980

Caries Res

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“…Caries scoring Caries was determined from the change in dentin fluorescence (Hietala et al, 1993) in a planimetric manner similar to that for dentinal apposition (Fig.). The hemisected dentitions were also stained with Schiff's reagent (pararosaniline), and the progression of caries lesions in the same molars described above was estimated by means of a modification of the method introduced by Konig et al (1958) and modified by Green and Hartles (1967). Lesions in the hemisection were scored as intact enamel lesions or dentinal lesions, according to the staining pattern.…”

Section: Predentin Widthmentioning

confidence: 99%

Evidence that High-sucrose Diet Reduces Dentin Formation and Disturbs Mineralization in Rat Molars

Hietala

Larmas

1995

J Dent Res

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In addition to its caries-promoting effect, a high-sucrose diet reduces the apposition of mineralized dentin in young rats. This study was undertaken to test whether it has a similar effect on the width of the as-yet-uncalcified matrix, predentin. Female Wistar rats were weaned at the age of 3 weeks and fed for 7 weeks with either a high-sucrose diet, a non-cariogenic raw potato starch diet, or a non-cariogenic commercial powdered rat food (for reference). The sucrose diet induced the greatest number of caries lesions. Dentin formation was smaller and the predentin zone wider in rats fed a sucrose diet when compared with rats fed the reference diet. In rats fed a starch diet, dentin formation was smaller than in rats fed a reference diet, but no alterations in the areas of caries lesions or predentin width were observed. Widening of the predentin zone in rats fed a high-sucrose diet may reflect changes in odontoblast function, such as reduced matrix synthesis and possibly disturbed mineralization. Dentinal caries progression may thus be modulated by odontoblast function, not as much by matrix formation but rather by mineralization.

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“…Smooth surface caries was scored according to Keyes' method [1958], The lower jaws were serially sectioned, stained and put on slides according to techniques de scribed previously [König et al, 1958;König, 1966;G reen and Hartles, 1967]. On the coded slides, the number of carious lesions in 16 fissures and the proximal areas of the right and left mandibular molars were counted under incident light at 16-times magnification.…”

Section: Methods Of Evaluationmentioning

confidence: 99%

Methodological Aspects of Gnotobiotic Caries Experimentation

Hoeven¹,

Mikx²,

Plasschaert³

et al. 1972

Caries Res

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A comparative study of caries activity is described on Osborne-Mendel rats reared germ-free and mono-associated with Strep, mutans OMZ 176 or E 49, or associated with one of these strains at the same time as the animals were conventionalized with an SPF microflora. The influence of the plaque matrix was studied with the aid of soft wood getting impacted in the fissures. Conventionalization with faeces from SPF rats resulted in a markedly lower caries activity compared with mono-infection. The presence of wood shavings in the diet and subsequent impaction in fissures did not result in increased caries activity when the flora consisted exclusively of microorganisms which formed sticky matrix polysaccharides.

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A modification to an established technique for the assessment of fissure caries in the rat

Cited by 24 publications

References 4 publications

Effect of Xylitol-Supplemented Diets on the Progression and Regression of Fissure Caries in the Albino Rat

Effect of Xylitol-Supplemented Diets on the Progression and Regression of Fissure Caries in the Albino Rat

Evidence that High-sucrose Diet Reduces Dentin Formation and Disturbs Mineralization in Rat Molars

Methodological Aspects of Gnotobiotic Caries Experimentation

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