Evidence that High-sucrose Diet Reduces Dentin Formation and Disturbs Mineralization in Rat Molars

Hietala, Eeva-Liisa; Larmas, Markku

doi:10.1177/00220345950740121401

Cited by 11 publications

(11 citation statements)

References 16 publications

(18 reference statements)

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“…2A,B) of the animals. Previous results suggest [3,4] that the reduction of dentin formation, caused by a high sucrose diet, might be a consequence of the increased width of predentin, which is a result of the disturbed mineralization of the dentin matrix. However, the results presented here not only demonstrate our working hypothesis to be indefensible, but also show that reduction of dentin apposition does not always agree with the increased width of predentin (Fig.…”

Section: Discussionmentioning

confidence: 96%

“…In order to measure the amount of dentin apposition (m 2 × 10 3 ), the main central transverse fissure of the first and second molars were each measured planimetrically using a microscope (Leica DMRB, Leica Mikroskopie und Systemic GmbH, D 35530 Wetzlar, Germany) equipped with a computer-connected video image analyzer (Leica Q 500 MC, Leica Cambridge Ltd, U.K.) and fluorescent light with which the tetracycline stripes surrounding the formed dentin could be seen [19] (magnification ×5). The width of the predentin layer (m) was measured under a microscope (magnification ×40) at six sites under the main fissures of the first and second molars, and the mean was considered to represent the thickness of predentin for that particular tooth [3].…”

Section: Sample Measurementsmentioning

confidence: 99%

“…We have observed that this diet resulted in a significant reduction in dentin apposition during the primary dentinogenesis of the rat [1][2][3][4][5]. The reducing effect seemed to be dose-dependent so that the higher the sucrose concentration was, the more reduced was the dentinogenesis [6].…”

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The Reducing Effects of a Calcium-Deficient Diet and High Sucrose Diet on Dentin Apposition of Rat Molars

et al. 2000

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A high sucrose diet reduces dentin apposition of growing rats. The mechanisms of reduction are unclear, but disturbances in calcium balance or in mineralization of predentin may explain them. In this experiment, 29 Sprague-Dawley rats, 21 days old, were weaned and randomized into calcium-deficient, high-sucrose or standard-diet groups for 3 weeks. They were given food and water ad libitum. During the experiment, animals were individually housed in metabolic cages where urine samples were collected. At ages of 21 and 40 days mineralizing dentin was marked using I.P. injections of oxytetracycline hydrochloride. At 42 days of age, the animals were anesthetized and their blood was collected by cardiac puncture. Right hemimandibles were sectioned sagittally and left hemimandibles were fixed, decalcified, and cut into histological sections. Dentin appositions were measured planimetrically, predentin width, from histological sections. Ca, K, and Na levels of serum and urine were measured flame photimetrically and P levels were measured by the UV method. Statistical analyses were done using one-way analysis of variation (ANOVA) Tuckey's HSD t test. In the calcium-deficient group, hypocalcemia, reduced dentin apposition, and increased predentin width were noticed when compared with the control group (P<0.05). Also, the increase in predentin width, caused by calcium deficiency, was significant compared with sucrose-fed animals (P<0.05). Sucrose diet reduced dentinogenesis, increased Ca excretion to urine, but also reduced urinary levels of P, K, and Na, and the differences were significant for the controls (P<0.05). In conclusion, despite the same kind of reduced dentinogenesis in calcium-deficient and high-sucrose groups, calcium imbalance or reduced mineralization of predentin does not explain reduced dentinogenesis in sucrose-fed animals.

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Section: Discussionmentioning

confidence: 96%

Section: Sample Measurementsmentioning

confidence: 99%

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The Reducing Effects of a Calcium-Deficient Diet and High Sucrose Diet on Dentin Apposition of Rat Molars

et al. 2000

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“…If the same applies to mature odontoblasts, the widening of the predentin may be caused by a decreased mineralization rate. Predentin width also increases with the impaired mineralization caused by a low‐calcium diet or a high‐sucrose diet , both resulting in a reduction in the rate of dentin formation.…”

Section: Growth Factors In Dentinmentioning

confidence: 99%

A review of the nature, role, and function of dentin non‐collagenous proteins. Part II: enzymes, serum proteins, and growth factors

Mazzoni¹,

Breschi²,

Carrilho³

et al. 2009

Endodontic Topics

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Part I was an overview of the role and function of proteoglycans and glycoproteins in the pulpo–dentin complex; part II will focus on enzymes, serum proteins, and growth factors. This review will discuss current knowledge regarding matrix metalloproteinases (MMPs), cathepsins, serum proteins, and growth factors in dentin and the related dentin–pulp complex in an attempt to better understand their nature, role, and function in the dentin extracellular matrix (ECM) environment. Dentin formation in physiological and pathological conditions has been widely studied. However, the regulation and involvement of non‐collageneous enzymes, serum proteins, and growth factors are still not completely elucidated. MMPs, a family of 23 endopeptidases in humans, are collectively capable of degrading virtually all ECM components, and their specific tissue inhibitors (TIMPs: tissue inhibitors of matrix metalloproteinases) participate in organo‐ and morphogenesis, physiological tissue turnover, and pathological tissue destruction. Similarly, the lysosomal cysteine proteinases (cathepsins) are capable of degrading ECM proteins such as collagen, laminin, fibronectin, and proteoglycans. These enzymes are implicated in a variety of pathological conditions, especially in diseases involving tissue re‐modeling states. Dentin also contains serum‐derived proteins (such as albumin, immunoglobulins, and transferrin), and a variety of growth factors in the mineralized ECM are available for release during demineralization or other injury. A detailed description of the components of the above‐mentioned dentin non‐collageneous proteins will be summarized in this literature review.

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“…The sections were stained with toluidine blue (STB). The width of the predentin was measured under a microscope at six sites under the main fissures of the first and second molars, and the mean was considered to represent the thickness for the particular group (20).…”

Section: Methodsmentioning

confidence: 99%

The Effect of Early Weaning on Dentin Formation and Dentinal Caries in Rats

Hietala¹,

Autio²,

Larmas³

1997

Acta Odontologica Scandinavica

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The effect of early weaning on caries progression, dentin formation, and dentin mineralization was examined in four groups of rats. Two groups received a normal diet and were weaned on day 18 or 21, and another two received a sucrose-rich diet and were weaned on day 18 or 21. At age 35 days the lower molars were sectioned sagittally, and the areas of dentin formation and of the dentinal caries were quantified. The width of the predentin zone was measured from histologically stained sections of maxillary molars. Early weaning reduced dentin formation in the group on the high-sucrose diet only the first days; later this effect was partially caught up with. A high-sucrose diet significantly increased caries frequency and extension of caries lesions compared with a normal diet in both early weaned and normally weaned groups. The effect of early weaning on caries frequency and extension in the high-sucrose group was insignificant compared with the normally weaned group on a high-sucrose diet. The predentin zone was wider in the sucrose groups than in the control groups at the end of the experiment. These results indicate that the effect of sucrose on dentin formation was dependent on the stage of physiologic dentin formation, but early weaning as such did not affect this.

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Evidence that High-sucrose Diet Reduces Dentin Formation and Disturbs Mineralization in Rat Molars

Cited by 11 publications

References 16 publications

The Reducing Effects of a Calcium-Deficient Diet and High Sucrose Diet on Dentin Apposition of Rat Molars

The Reducing Effects of a Calcium-Deficient Diet and High Sucrose Diet on Dentin Apposition of Rat Molars

A review of the nature, role, and function of dentin non‐collagenous proteins. Part II: enzymes, serum proteins, and growth factors

The Effect of Early Weaning on Dentin Formation and Dentinal Caries in Rats

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