The Effect of Early Weaning on Dentin Formation and Dentinal Caries in Rats

Hietala, Eeva-Liisa; Autio, Jaana; Larmas, Markku

doi:10.3109/00016359709115417

Cited by 5 publications

(2 citation statements)

References 18 publications

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“…A major issue of in vivo procedures, however, is the inconsistency of the parameters and factors that induce caries formation (Bowen 2013). In particular, the lack of details about the cariogenic diet and feeding conditions (Guggenheim et al 1966; Navia et al 1969; Firestone et al 1982), infectious organism (Willcox et al 1989; Klinke et al 2011), age of animals (Hietala et al 1997; Bao et al 2015), and housing conditions (Schuster et al 1978) often limits the reproducibility of these procedures (Bowen 2013).…”

Section: Introductionmentioning

confidence: 99%

Early Caries in an In Vivo Model: Structural and Nanomechanical Characterization

et al. 2018

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The utilization of rat models in cariology research has made substantial contributions to decipher mechanisms of caries formation and to develop preventive treatments. The existing rat models still have potential for improvement toward establishing a more accurate standard caries protocol to utilize in testing and/or developing new dental technologies. The current caries-scoring methods rely on optical microscopy-based techniques, which necessitates formation of highly advanced lesions. Moreover, models that facilitate the implementation of cariogenic bacteria by shifting the balance of oral flora through desalivation and/or antibiotic treatment create a nonnatural environment. Furthermore, there is a paucity of detailed structural and mechanical characterization on the resulting carious lesions. The purpose of this study was to develop a rat model that induces formation of mild carious lesions and to provide comprehensive structural and mechanical characterization. With this aim in mind, an in vivo model promoting progression of mild lesions was established with specific pathogen-free Sprague-Dawley rats. Cariogenic bacteria, Streptococcus mutans, was implemented into the oral flora without the use of antibiotics or desalivation surgery. During caries formation, progression of the infection was monitored by quantifying the relative abundance of S. mutans in oral flora with quantitative real-time polymerase chain reaction. A significant increase in colonization efficacy of S. mutans was detected during cariogenic challenge ( P < 0.01). The resulting carious lesions were analyzed by conventional light optical and scanning electron microscopy. A detailed structural and morphological characterization on fissure caries with different degrees of severity was provided. The changes in the morphology and demineralization state of the sound and carious tissues were quantified by energy-dispersive X-ray spectroscopy, and local mechanical properties were acquired with nanoindentation. The principles laid out in this work can be utilized in cariology research and developed into a standard protocol for future studies.

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Section: Introductionmentioning

confidence: 99%

Early Caries in an In Vivo Model: Structural and Nanomechanical Characterization

et al. 2018

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“…it does not reduce the speed of dentin mineralization. Moreover, the reductive effect of a high sucrose diet on the dentinogenesis of young rats is predisposed by the sucrose feeding of their dams during the preweaning period (11), just as is early weaning of rat pups (12). Although all previous results suggest that the effect of the diet is mediated via the response of the host during primary dentinogenesis, the mechanism by which a high sucrose diet reduces dentin formation is still unknown.…”

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confidence: 96%

Effects of a high sucrose diet and intragastric sucrose feeding on the dentinogenesis, dental caries, and mineral excretion of the young rat

Pekkala

Hietala

Puukka

et al. 2000

Acta Odontologica Scandinavica

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Previous studies show that a high sucrose diet reduces the rate of primary dentinogenesis and increases dental caries, although their cause-effect relationship is still obscure. The purpose of this study was to explore whether the effect of sucrose load on the dentinogenesis and dental caries of young rat molars is mediated by systemic (intragastric) or by systemic and local (dietary) factors. At weaning (19 days), animals were randomized into the control, intragastric sucrose, and dietary sucrose groups for 4 weeks. The areas of dentin appositions and dentinal caries lesions were measured planimetrically. Caries was also determined with Shiffs staining and the width of predentin by histology. Urinary Ca, K, and Na levels were measured by flame photometry, urinary P levels using an UV method, and serum insulin levels using radioimmunoassay. Systemic and local sucrose load reduced dentin appositions and intragastric sucrose increased urinary Ca excretion. No differences in the width of predentin were noticed. Only dietary sucrose enhanced the occurrence and progression of caries. The present findings show that sucrose load reduces dentinogenesis by impairing the synthesis of dentin matrix, but also point out the crucial importance of the local sucrose challenge in the initiation of dental caries.

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Effects of protein deficiency induced by raw soy with and without sucrose on dentine formation and dentinal caries in young rats

Huumonen

Larmas

2005

Archives of Oral Biology

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The Effect of Early Weaning on Dentin Formation and Dentinal Caries in Rats

Cited by 5 publications

References 18 publications

Early Caries in an In Vivo Model: Structural and Nanomechanical Characterization

Early Caries in an In Vivo Model: Structural and Nanomechanical Characterization

Effects of a high sucrose diet and intragastric sucrose feeding on the dentinogenesis, dental caries, and mineral excretion of the young rat

Effects of protein deficiency induced by raw soy with and without sucrose on dentine formation and dentinal caries in young rats

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