A modified acid-fast staining method for rapid detection of Mycobacterium tuberculosis

Zhao, Dan; Yang, Xiaomeng; Chen, Qian-Yu; Zhang, Xingshu; Chun-jiang, Guo; Che, Xin

doi:10.1016/j.mimet.2012.07.024

Cited by 15 publications

(8 citation statements)

References 7 publications

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“…The Ziehl-Neelsen (ZN) staining had traditionally been used as a standard method for detecting MTB (all forms) in TB samples. Recently, reports have leveraged the emergence of Intensified Kinyoun's (IK) acid-fast staining technique to detect MTB in general, and more specifically the L forms [10, 14]. …”

Section: Resultsmentioning

confidence: 99%

Clinical End-Points Associated withMycobacterium tuberculosisand Lung Cancer: Implications into Host-Pathogen Interaction and Coevolution

Tian

Hao

Cao

et al. 2015

BioMed Research International

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There is a recent emerging theory that suggests a cross-link between pathogens and cancer. In this context, we examined the association between the Mycobacterium tuberculosis (MTB) with its L-forms (MTB-L) and lung cancer. In the present study, we have optimized and applied a highly sensitive assay to detect the presence of MTB and MTB-L in 187 lung cancer samples and 39 samples of other cancer origins. By carefully controlling confounding factors, we have found that 62% of the lung cancer samples are MTB-L positive, while only 5.1% of the other cancer samples are MTB-L positive. Through generalized linear models and random forest models, we have further identified a set of clinical end-points that are strongly associated with MTB-L presence. Our finding provides the basis for future studies to investigate the underlying mechanism linking MTB-L infection to lung cancer development.

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Section: Resultsmentioning

confidence: 99%

Clinical End-Points Associated withMycobacterium tuberculosisand Lung Cancer: Implications into Host-Pathogen Interaction and Coevolution

Tian

Hao

Cao

et al. 2015

BioMed Research International

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“…Moreover, the added heat in the procedure enhanced the penetration of the carbol fuchsin dye through the bacterial cell wall and into the cytoplasm. 9,15,20,21 Using hydrochloric acid instead of sulfuric acid as a decolorizing agent also showed to be effective. The Revised National Tuberculosis Programme recommends the use of sulfuric acid as the decolorizing agent as it easily removes background material even from thick smears making identification of AFB easier.…”

Section: Discussionmentioning

confidence: 99%

Alternative Method for Histopathological Detection of Mycobacterium Tuberculosis and Mycobacterium Leprae using a Modified Acid-Fast Technique

Cabic¹,

Cabic²,

Esposo³

et al. 2018

PJP

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Introduction. Mycobacterium tuberculosis and Mycobacterium leprae are acid-fast organisms with lipid-rich cell walls that resist decolorization with acidified alcohol after application of a dye with heat. The Ziehl-Neelsen and Fite Faraco staining technique, which are diagnostic tools for identification of acid-fast bacilli (AFB) found in histopathologic samples, are based on this principle. A modification of the Ziehl-Neelsen technique is described as an alternative rapid and reliable method of diagnosis for prompt detection and treatment.Methodology. One hundred and seven (107) archived tissue specimens from autopsy and dermatology cases interpreted as positive for M. tuberculosis and M. leprae were stained using the proposed modified acid-fast (MAF) technique compared with Fite Faraco (FF) staining method as reference standard. Each specimen was read by two independent evaluators.Results. The degree of diagnostic agreement of the MAF with FF was calculated. For autopsy (n=16) and dermatology (n=91) samples, the Cohen's kappas are 0.765 (substantial) and 0.397 (fair), respectively. Overall, the Cohen's kappa is 0.458 (moderate). Conclusion.The proposed modified Acid-Fast staining method may be considered as an alternative to the conventional Ziehl-Neelsen method and the Fite Faraco method in identifying positive acid-fast bacilli in tissue samples taken from clinical cases of M. tuberculosis and M. leprae.

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“…After incubation on BHI agar at 28 °C for 3 days, cell morphology was observed under a light microscope (Eclipse 80i) and a transmission electron microscope (HT7700), Gram-staining was performed using a Gram-stain kit according to the manufacturer's instructions and acid-fast staining (AFS) was performed with an AFS kit using a modified Ziehl-Neelsen method [32]. Anaerobic growth on BHI agar was tested by incubation in an anaerobic chamber for up to 10 days.…”

Section: Phenotypic Physiological and Biochemical Analysesmentioning

confidence: 99%

Mycolicibacterium baixiangningiae sp. nov. and Mycolicibacterium mengxianglii sp. nov., two new rapidly growing mycobacterial species

Cheng

Lei

Wang

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Four bacterial strains (LJ126T/S18 and Z-34T/S20) recovered from faecal samples of Tibetan antelopes on the Qinghai–Tibet Plateau of China were analysed using a polyphasic approach. All four isolates were aerobic, short rod-shaped, non-motile, Gram-stain-positive, acid-fast and fast-growing. Phylogenetic analyses based upon 16S rRNA and whole-genome sequences showed that the two pair of strains formed two distinct branches within the evolutionary radiation of the genus Mycolicibacterium . Strains LJ126T/S18 and Z-34T/S20 were most closely related to Mycolicibacterium austroafricanum CCUG 37667T, Mycobacterium aurum NCTC 10437T, Mycobacterium pyrenivorans DSM 44605T, Mycobacterium monacense JCM 15658T, Mycolicibacterium sarraceniae JCM 30395T, Mycolicibacterium tokaiense JCM 6373T and Mycobacterium murale JCM 13392T, but readily distinguished from the known species by a combination of chemotaxonomic and phenotypic features and by low average nucleotide identity values (74.4–84.9 %). Consequently, the two strain pairs are considered to represent different novel species of Mycolicibacterium for which the names Mycolicibacterium baixiangningiae sp. nov. and Mycolicibacterium mengxianglii sp. nov. are proposed, with LJ126T (=CGMCC 1.1992T=KCTC 49535T) and Z-34T (=CGMCC 1.1993T=DSM 106172T) as the respective type strains.

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A modified acid-fast staining method for rapid detection of Mycobacterium tuberculosis

Cited by 15 publications

References 7 publications

Clinical End-Points Associated withMycobacterium tuberculosisand Lung Cancer: Implications into Host-Pathogen Interaction and Coevolution

Clinical End-Points Associated withMycobacterium tuberculosisand Lung Cancer: Implications into Host-Pathogen Interaction and Coevolution

Alternative Method for Histopathological Detection of Mycobacterium Tuberculosis and Mycobacterium Leprae using a Modified Acid-Fast Technique

Mycolicibacterium baixiangningiae sp. nov. and Mycolicibacterium mengxianglii sp. nov., two new rapidly growing mycobacterial species

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