A modified, economic, sensitive method for measuring total antioxidant capacities of human plasma and natural compounds using Indian saffron (Crocus sativus)

Chatterjee, Saurabh; Poduval, T. Balakrishna; Tilak, Jai C.; Devasagayam, T.P.A.

doi:10.1016/j.cccn.2004.09.012

Cited by 55 publications

(50 citation statements)

References 22 publications

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“…Psoralea corylifolia (Maharik et al 2009), have been documented. However, there are also many reports documenting the role of crocin compounds in inhibiting as well as scavenging free radicals (Fernandez 2006), modulating lipid peroxidation (Chatterjee et al 2005), or protecting DNA and tRNA from harmful chemical reactions (Limaye et al 2003).…”

Section: Antioxidant Activitymentioning

confidence: 99%

Antibacterial and antioxidant activity of methanol extracts ofCrocus sativusL.c.v. Kashmirianus

Parray

Kamili

Hamid

et al. 2014

Frontiers in Life Science

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In this study, we investigated the antibacterial and antioxidant activities of Crocus sativus L. Kashmirianus c.v. extracts (callus and stigmas). Profuse callus was obtained on MS medium enriched with BAP (20 µM) + NAA(15 µM) under in vitro conditions from corm slices. Four pathogenic bacterial strains (Staphylococcus aureus CD0001, Escherichia coli CD0006, Pseudomonas aeruginosa CD0023 and Shigella flexneri CD0033) were used for determining the antibacterial activity of extracts. The antioxidant activity was determined by DPPH assay, DNA protection assay, FTC method, TBA assay and lipid peroxidation assay. The methanol stigma extract of saffron was found to be more effective in inhibiting all the pathogenic strains. The stigma extract also showed significant radical scavenging or chelation capacities in four of the methods; however, callus extract exhibited maximum inhibition of peroxy-radicals in lipid peroxidation assay. The protocol for callus production is described. It was concluded that as well as the specific parts of plants displaying diverse pharmacological activities, callus produced under in vitro conditions will assist in enhancing the production of secondary metabolites, which will reduce the pressure on natural saffron.Keywords: antioxidant activity; callus; in vitro culture; MIC; methanol; saffron; stigma Abbreviations: BAP = 6-benzyl adenine purine BHT = butylated hydroxyl toluene FTC = ferric thiocyanate MIC = minimum inhibitory concentration NAA = naphthalene acetic acid TBA = thiobarbituric acid.

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Section: Antioxidant Activitymentioning

confidence: 99%

Antibacterial and antioxidant activity of methanol extracts ofCrocus sativusL.c.v. Kashmirianus

Parray

Kamili

Hamid

et al. 2014

Frontiers in Life Science

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show abstract

“…[53][54][55][56] Acorus calamus, 57) Asparagus racemosus, 58) Foeniculum vulgare, 40) Celastrus paniculatus, 19) Centella asiatica, 59) Crocus sativus, 60) Cyperus rotundus, 61) Elettaria cardamomum, 62) Phyllanthus emblica, 63) Glycyrrhiza glabra, 64) Nardostachys jatamansi, 65) Ocimum sanctus, 49) Piper longum, 66) Terminalia chebula, 67) Zingiber officinales 68) and Withania somnifera 69) are ingredients of polyherbal product Abana, have been reported to possess antioxidant property, which susceptible brain cells get exposed to less oxidative stress resulting in reduced brain damage and improved neuronal function.…”

Section: Discussionmentioning

confidence: 99%

Memory Enhancing Activity of Abana: An Indian Ayurvedic Poly-Herbal Formulation

Parle

Mani

2007

JOURNAL OF HEALTH SCIENCE

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The present study was aimed at investigating the effects of Abana, an Ayurvedic herbomineral preparation on memory and brain cholinesterase activity in mice. Drug Abana was administered orally in three doses (50, 100 and 200 mg/kg) for fifteen days to different groups of young and aged mice. Elevated plus maze and passive avoidance apparatus served as the exteroceptive behavioral models for testing memory. Diazepam-, scopolamine-and ageinginduced amnesia served as the interoceptive behavioral models. Brain cholinesterase activity was also estimated. Abana (50, 100 and 200 mg/kg, orally (p.o.)) produced a dose-dependent improvement in memory scores of young and aged mice. Furthermore, it reversed the amnesia induced by scopolamine (0.4 mg/kg, intraperitoneally (i.p.)) and diazepam (1 mg/kg, i.p.). Interestingly, brain cholinesterase activity was reduced by Abana administered orally for 15 days. It may prove to be a useful remedy for the management of Alzheimer's disease.

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“…Clinical trials and epidemiological studies have established an inverse correlation between the intake of natural exogenous antioxidants and the occurrence of oxidative stress diseases such as inflammation, cardiovascular problems, cancer, and aging-related disorders (Gutteridge & Halliwell, 2010). Thus, the analysis of natural antioxidants for disease prevention (Chatterjee et al, 2005;Notas et al, 2005) and the identification of possible pro-oxidant substances have become topics of increasing interest.…”

Section: Introductionmentioning

confidence: 99%

Analytical criteria to quantify and compare the antioxidant and pro-oxidant capacity in competition assays: The bell protection function

Prieto¹,

Murado²,

Vázquez³

et al. 2014

Food Research International

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Publication informationFood Research International, 60 (2014): 48-58 Publisher ElsevierItem record/more information http://hdl.handle.net/10197/6178 Publisher's statement þÿ T h i s i s t h e a u t h o r s v e r s i o n o f a w o r k t h a t w a s a c c e p t e d f o r p u b l i c a t i o n i n F o o d R e s e a r c hInternational. Changes resulting from the publishing process, such as peer review, editing, ABSTRACTThe development of a convenient mathematical application for testing the antioxidant and prooxidant potential of standard and novel therapeutic agents is essential for the research community and food industry in order to perform more precise evaluations of products and processes. In this work, a simple non-linear dose-time tool to test the effectiveness of compounds for competitive assays is presented. The model helps to describe accurately the antioxidant and pro-oxidant response as a function of time and dose by two criteria values and allows one to perform easily comparisons of both capacities from different compounds. The quantification procedure developed was applied to two well known in vitro competition assays, the β-carotene and crocin bleaching asymptotic reactions. The dose-time dependency of the response of commercial antioxidants and some expected pro-oxidant compounds was evaluated in this study and the results showed low experimental error. In addition, as an illustrative example of the capabilities of the criteria proposed, the quantification of the combined effect of an antioxidant and a pro-oxidant was analyzed. Afterwards, the model was verified for other relevant competitive methods, using available experimental data from the bibliography. Its application is simple, it provides parametric estimates which characterize the response, and it facilitates rigorous comparisons among the effects of different compounds and experimental approaches. In all experimental data tested, the calculated parameters were always statistically significant (Student's t-test, α = 0.05), the equations were consistent (Fisher's F-test) and the goodness of fit coefficient of determination was higher than 0.98.

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A modified, economic, sensitive method for measuring total antioxidant capacities of human plasma and natural compounds using Indian saffron (Crocus sativus)

Cited by 55 publications

References 22 publications

Antibacterial and antioxidant activity of methanol extracts ofCrocus sativusL.c.v. Kashmirianus

Antibacterial and antioxidant activity of methanol extracts ofCrocus sativusL.c.v. Kashmirianus

Memory Enhancing Activity of Abana: An Indian Ayurvedic Poly-Herbal Formulation

Analytical criteria to quantify and compare the antioxidant and pro-oxidant capacity in competition assays: The bell protection function

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