A Modified Method for the Determination of 2,3-Diphosphoglycerate in Erythrocytes

Ericson, Åke; Verdier, C.-H. De

doi:10.3109/00365517209081059

Cited by 145 publications

(27 citation statements)

References 7 publications

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“…Aliquots of the blood were also extracted for the enzymatic We report here a family with chronic haemolysis and isodetermination of 2,3-diphosphoglycerate (2,3-DPG) 9 as lated accumulation of erythrocyte CDP-choline as detected described earlier. 8 Pyrimidine 5′-nucleotidase activity was and identified by 31 P-NMR spectroscopy.…”

Section: Preparation Of Blood For Investigationmentioning

confidence: 89%

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A family with chronic haemolysis and selective accumulation of erythrocyte CDP-choline

Petersen

Borregaard

1997

Leukemia

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Section: Preparation Of Blood For Investigationmentioning

confidence: 89%

“…2,3-DPG was determined enzycomplicated right crural fracture at age 16, the clinical history matically. 9 The 31 P-NMR spectra were referenced to 85% was uneventful. The following laboratory tests were found to …”

Section: Methodsmentioning

confidence: 99%

A family with chronic haemolysis and selective accumulation of erythrocyte CDP-choline

Petersen

Borregaard

1997

Leukemia

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“…2,3-DPG concentration was determined according to Ericson and de Verdier [5], whereas the concentrations of other internal phosphates-G-6-P, ATP, and ADP--were measured according to Minakami et al [6]. In order to measure low concentrations reproducibly a sensitive Cary 218 photometer was used.…”

Section: Methodsmentioning

confidence: 99%

“…The assay composition was as follows (final concentrations in a total volume of 2.0 ml): 100 mmol/l iter triethanolamineiHC1 0. 5 The parameter describing cooperativity (nH) was obtained from the well-known Hill plot, whereas a Scatchard plot [ 141 gave additional information about positive, negative, or mixed cooperativity .…”

Section: Methodsmentioning

confidence: 99%

Erythrocyte pyruvate kinase deficiency: A kinetic method for differentiation between heterozygosity and compound‐heterozygosity

et al. 1989

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The goal of the present study was to search for criteria that allow one to distinguish between normal individuals and heterozygotes as well as compound heterozygotes for pyruvate kinase (PK) deficiency. As the residual activity of PK with heterozygotes was between 35% and 110% of the normal activity, it was necessary to find other methods to prove heterozygosity. The PK in the hemolysates of 23 patients suffering from PK deficiency, 36 paternal and maternal enzymes as well as the enzymes of five heterozygous and four normal siblings together with those of 20 normal individuals, were studied according to the recommendations of the International Committee for Standardization in Haematology. The following hematological and enzyme kinetic parameters can serve to identify heterozygotes for PK deficiency: 1) a slight reticulocytosis, 2) an up-to-twofold increase of the intracellular concentrations of glucose-6-phosphate in the erythrocyte, 3) a mixed cooperativity of the phosphoenolpyruvate (PEP)-binding process of PK, 4) a decreased nucleotide specificity with guanosine diphosphate and uridine diphosphate, and 5) a lowered affinity for adenosine diphosphate. The most significant criterium found with all heterozygotes was a mixed cooperativity of the PEP-binding process caused by the presence of a mixture of normal and mutant PK.

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“…Red cell 2,3-DPG concentration was determined by an enzymatic method according to Ericsson and de Verdier [11]. The inter-assay variation was 2.1%.…”

Section: Methodsmentioning

confidence: 99%

Correlation of serum unconjugated oestriol to red cell 2,3-diphosphoglycerate levels in diabetic pregnancy

Madsen

Ditzel

1983

Diabetologia

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Summary. In order to evaluate the possible underlying factors for the increase in red cell 2,3-diphosphoglycerate content observed in late diabetic pregnancy, its relationship with serum unconjugated oestriol, human placental lactogen, haemoglobin and hydrogen ion concentrations was investigated in 42 pregnant diabetic women. A significant correlation was found between red cell 2,3-diphosphoglycerate and serum unconjugated oestriol (r= 0.54, p < 0.001), whereas no correlation was present between 2,3-diphosphoglycerate and the following variables: arterial pH, haemoglobin concentration and human placental lactogen. The content of 2,3-diphosphoglycerate correlated significantly with haemoglobin-oxygen affinity expressed as Ps0 at pH 7.4 (r= 0.34, p < 0.05). The results of this study indicate that serum unconjugated oestriol may participate in the regulation of red cell 2,3-diphosphoglycerate content and thereby of the maternal blood oxygen release to the fetus.

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A Modified Method for the Determination of 2,3-Diphosphoglycerate in Erythrocytes

Cited by 145 publications

References 7 publications

A family with chronic haemolysis and selective accumulation of erythrocyte CDP-choline

A family with chronic haemolysis and selective accumulation of erythrocyte CDP-choline

Erythrocyte pyruvate kinase deficiency: A kinetic method for differentiation between heterozygosity and compound‐heterozygosity

Correlation of serum unconjugated oestriol to red cell 2,3-diphosphoglycerate levels in diabetic pregnancy

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