A modified procedure for staining roots to detect VA mycorrhizas

Koske, R. E.; Gemma, J. N.

doi:10.1016/s0953-7562(89)80195-9

Cited by 1,348 publications

(262 citation statements)

References 4 publications

Supporting

Mentioning

259

Contrasting

Unclassified

Order By: Relevance

“…For assessing mycorrhizal infection, root samples were collected and stained with 0.05% trypan blue in lactic acid (Phillips and Hayman, 1970) modified by the procedure described by Koske and Gemma (1989). The percentage of root colonization was determined using the grid line intersect method (Giovanetti and Mosse, 1980).…”

Section: Methodsmentioning

confidence: 99%

Interaction between the root-lesion nematode Pratylenchus vulnus and the mycorrhizal association of Glomus intraradices and Santa Lucia 64 cherry rootstock

Pinochet¹,

Calvet²,

Camprubí³

et al. 1995

Plant Soil

View full text Add to dashboard Cite

The effects of the interaction between Pratylenchus vulnus and the endomycorrhizal fungus Glomus intraradices on growth and nutrition of Santa Lucia 64 cherry rootstock was studied under microplot conditions during one growing season. Fresh top weight, and stem diameter of mycorrhizal plants and high P treatments with and without P vulnus were significantly higher than those of non-mycorrhizal plants. The lowest shoot length and fresh root weights were recorded in nematode inoculated plants in low P soil. Mycorrhizal infection did not affect the number of nematodes per gram of root in plants infected with P vulnus. In the presence of the nematode, internal spore production by G. intraradices was significantly reduced. No nutrient deficiencies were detected through foliar analysis, although low levels of Ca, Mn and Fe were detected in nematode treatments. Mycorrhizal plants achieved the highest values for N, P, S, Fe, and Zn, whereas high P treatments increased absorption of Ca and Mn. Early mycorrhizal infection of Santa Lucia 64 cherry rootstock by G. intraradices confers increased growth capacity in the presence of P vulnus.

show abstract

Section: Methodsmentioning

confidence: 99%

Interaction between the root-lesion nematode Pratylenchus vulnus and the mycorrhizal association of Glomus intraradices and Santa Lucia 64 cherry rootstock

Pinochet¹,

Calvet²,

Camprubí³

et al. 1995

Plant Soil

View full text Add to dashboard Cite

show abstract

“…Assessments of percentage mycorrhizal colonisation were made on ten secondary roots and their associated higher orders, chosen randomly from each of the three primary roots selected for morphological characterisation. Roots were stained with trypan blue (Koske and Gemma, 1989) and estimates of percentage colonisation of the root system and of different root orders made using a grid intersect method (Giovannetti and Mosse, 1978). Total root length of plants from another block were measured using Magiscan.…”

Section: Methodsmentioning

confidence: 99%

Vesicular-arbuscular mycorrhizal fungi induced alteration in poplar root system morphology

1992

View full text Add to dashboard Cite

Poplar was grown in a soil either inoculated with Scutellispora calospora, Glomus sp E3 or Glomus caledonium or to which a nutrient solution had been added, in order to determine effects on root morphology. Plants were harvested after 115 days. The lengths of individual roots were measured using image analysis and percentage colonisation was determined for different root orders. Colonisation did not affect plant size but induced large changes in root morphology, with lengths of individual secondary and tertiary roots increased in some cases by up to 100%. Root branching was also increased with number of laterals per unit length of colonised roots being up to 6 times greater than in non-colonised roots. These results clearly show that colonisation of roots by Vesicular-arbuscular mycorrhizal fungi can result in significant alteration to poplar root system morphology. They also suggest that the mechanisms of alteration are not entirely due to improved host plant nutrition.

show abstract

“…A proportion of the preserved root was stained and examined for the presence and degree of mycorrhizal infection. The root clearing and staining procedure followed the method of Koske and Gemma (1989). The percentage mycorrhizal infection was calculated as the number of 10 mm long root segments out of 100 identified as infected under a stereo microscope at a magnification of x 20 (Giovannetti and Mosse, 1980).…”

Section: Mycorrhizal Infectionmentioning

confidence: 99%

Enhanced uptake of phosphorus by mycorrhizal sorghum plants as influenced by forms of nitrogen

1996

View full text Add to dashboard Cite

The effects of NH+-N and NO~--N applications on rhizosphere pH and P uptake were investigated with and without mycorrhizal inoculation. Sorghum (Sorghum bicolor L.) plants were grown in split pots and in different soils with different initial soil pH values and P contents. In both soils, ammonium treatment resulted in higher plant dry weight and P content than nitrate treatment at all P levels. Mycorrhizal inoculation enhanced the differences. Sorghum plants acidified the rhizosphere at low soil P status with both nitrogen forms, and with or without mycorrhiza. When N was supplied as NO~-N, rhizosphere pH increased gradually with increasing P addition.The experiments showed that, although the zone of strong acidification coincided with the main zone of P depletion, the contribution of Arbuscular Mycorrhizal Fungi (AMF) to P uptake extended beyond the effect of rhizosphere pH change. There were no differences in pH change between mycorrhizal and non-mycorrhizal inoculated soils with the same N form, and it appears that pH change is an independent factor affecting P uptake regardless of whether the plant root is infected or not. The differences in soil pH change with N form do not however explain sufficiently the differences in dry matter increase and P uptake between mycorrhizal and non-mycorrhizal plants.

show abstract

A modified procedure for staining roots to detect VA mycorrhizas

Cited by 1,348 publications

References 4 publications

Interaction between the root-lesion nematode Pratylenchus vulnus and the mycorrhizal association of Glomus intraradices and Santa Lucia 64 cherry rootstock

Interaction between the root-lesion nematode Pratylenchus vulnus and the mycorrhizal association of Glomus intraradices and Santa Lucia 64 cherry rootstock

Vesicular-arbuscular mycorrhizal fungi induced alteration in poplar root system morphology

Enhanced uptake of phosphorus by mycorrhizal sorghum plants as influenced by forms of nitrogen

Contact Info

Product

Resources

About