A Modified Protocol for Agrobacterium Mediated Transformation of Tomato Plants

Mishra, Pranav; Pillai, H. P. Jai Shanker; Kopa, Herman; Gupta, Megha; Arjunan, Selvam

doi:10.20546/ijcrbp.2017.401.016

Int. J. Curr. Res. Biosci. Plant Biol.

2017

DOI: 10.20546/ijcrbp.2017.401.016

|View full text |Cite

A Modified Protocol for Agrobacterium Mediated Transformation of Tomato Plants

Pranav Mishra¹,

H. P. Jai Shanker Pillai²,

Herman Kopa³

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2021

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Agrobacterium-Mediated Transformation of Two Tomato Cultivars (Lycopersicon Esculentum Mill.) Cv. Sandra and Rocky

Danial¹,

Ibrahim²,

Song³

2021

Iraqi J. Agric. Sci.

View full text Add to dashboard Cite

An efficient protocol for Agrobacterium-mediated transformation of tomato cultivars Sandra and Rocky was conducted to examine the possibility of producing transgenic tomato plants cultivars harbouring the nptII gene, conferring kanamycin resistance. To achieve this aim, tomato cotyledon explants were transformed using EHA105 Agrobacterium tumefaciens strain harboring the binary vectors pBI121 which contains Gus gene, and neomycin phosphotransferase II (nptII) as selectable marker gene under the control of a CaMV35S promoter and nopaline synthase (nos) Terminator. Transformant detection was carried out in three distinct ways. First antibiotic selection, Kanamycin at a concentration of100 mgl-1 found to be efficient for this purpose. Second histochemical GUS assay revealed the presence of blue colored zones in a number of shoots and leaves for both in vitro and the greenhouse-grown transgenic plants. Third PCR analysis indicated positive result by showing the fragment for nptII gene in tested transformants, while was absent in non-transgenic control (wild type). On the other hand, the results showed that Sandra cultivar was more efficient for regeneration and subsequently transformation frequency than Rocky cultivar, which record 26.66% of transformation frequency compared with 11.57% in Rocky cultivar.

show abstract

Agrobacterium-Mediated Transformation of Two Tomato Cultivars (Lycopersicon Esculentum Mill.) Cv. Sandra and Rocky

Danial¹,

Ibrahim²,

Song³

2021

Iraqi J. Agric. Sci.

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

A Modified Protocol for Agrobacterium Mediated Transformation of Tomato Plants

Cited by 1 publication

References 0 publications

Agrobacterium-Mediated Transformation of Two Tomato Cultivars (Lycopersicon Esculentum Mill.) Cv. Sandra and Rocky

Agrobacterium-Mediated Transformation of Two Tomato Cultivars (Lycopersicon Esculentum Mill.) Cv. Sandra and Rocky

Contact Info

Product

Resources

About