A modified protocol for in vitro maturation of mouse oocytes from secondary preantral follicles

Tang, Y.; Wang, W.; Ni, Lujuan; Liu, H.-C.

doi:10.1016/j.fertnstert.2011.07.924

Cited by 8 publications

(10 citation statements)

References 93 publications

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“…This increase seems to be related to the accumulation of follicular fluid during follicle maturation process in folliculogenesis. This process is mediated primarily by gonadotropic hormones, local development and growth factors that participate in autocrine and paracrine regulation in these events [30]- [32]. Activin is one of these growth factors.…”

Section: Discussionmentioning

confidence: 99%

High Levels Production of Recombinant Human Activin A—Effect upon <i>in Vivo</i> Follicle Stimulation

Souza¹,

Almeida²,

Araujo³

et al. 2015

ABB

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Activin plays an important role in numerous physiological processes such as cell differentiation and remodeling, regeneration and repair of tissues from various organs, angiogenesis, morphogenesis of glandular organs, pluripotency and differentiation of stem cells, cell adhesion and apoptosis. It participates in reproductive processes like embryogenesis, in the expression of Follicle Stimulating Hormone and Luteinizing Hormone and maturation of ovarian follicles and therefore has application in the area of reproduction of vertebrates. Given the economic importance of activin, we develop an efficient and economical method for the production of recombinant human activin A (rACT), using as expression system the yeast Pichia pastoris. rACT showed biological activity as it induced, on submicromolar dose, the increase of ovarian mass and the ovulation process in a mammal model.

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Section: Discussionmentioning

confidence: 99%

High Levels Production of Recombinant Human Activin A—Effect upon <i>in Vivo</i> Follicle Stimulation

Souza¹,

Almeida²,

Araujo³

et al. 2015

ABB

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“…Although this conventional HD method works effectively for the in vitro culture of the secondary preantral follicles of inbred mice, 36 it is apparently not directly transferrable to outbred deer mice. Therefore, we modified the conventional method by continuously culturing the aggregated follicles in HD on day 7-13.…”

Section: In Vitro Follicle Culture: the Conventional MD Methodsmentioning

confidence: 99%

“…The former is the same as that reported in the literature for inbred mice by transferring the aggregates into 30 mL drops (one aggregate per drop) of the nonconditioned culture medium covered with mineral oil. 36 The modified method consisted of further culturing the aggregates in 30 mL HDs of the nonconditioned culture medium (also, one aggregate per HD) with half of the medium (15 mL) in each drop being replaced with the fresh medium every other day till day 13.…”

Section: Preparation Of Ovarian Cell-cmmentioning

confidence: 99%

“…34,35 Inspired by studies culturing embryonic stem cells to form an embryoid body, a recent study reported in vitro culture of preantral follicles from inbred mice in hanging drop (HD) and found it being better than the conventional 2D and even the 3D alginate hydrogel systems. 36 This could be attributed partially to the nearly zero compressive stress on the growing follicles in the fluidic HD of the culture medium.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Culture of Early Secondary Preantral Follicles in Hanging Drop of Ovarian Cell-Conditioned Medium to Obtain MII Oocytes from Outbred Deer Mice

Choi

Agarwal

2013

Tissue Engineering Part A

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“…In the past decades, studies have been reported to culture (mainly preantral) follicles on the 2D flat surface of culture dish [9,10], in homogeneous 3D hanging drop [11], and in homogeneous 3D hydrogel of alginate or an interpenetrating network of alginate and fibrin [12–17]. Moreover, results from some of the past studies demonstrate the potential of in vitro follicle culture for restoring or preserving fertility as live birth of offspring has been achieved using this approach [18–21].…”

Section: Introductionmentioning

confidence: 99%

In vitro culture of ovarian follicles from Peromyscus

Toth

2017

Seminars in Cell & Developmental Biology

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The ovarian follicle is the fundamental functional tissue unit of mammalian ovary. Each ovarian follicle contains one single oocyte. Isolation and in vitro culture of ovarian follicles to obtain fertilizable oocytes have been regarded as a promising strategy for women to combat infertility. The follicles from Peromyscus are considered as a better model than that from inbred mice for studying follicle culture. This is because Peromyscus mice are outbred (as with humans) with an increased life span. In this article, we reviewed studies on this subject conducted using Peromyscus follicles. These studies show that the conventional 2D micro-drop and 3D hanging-drop approaches established for in vitro culture of early preantral follicles from inbred mice are not directly applicable for cultivating the follicles from Peromyscus However, the efficiency could be significantly improved by culturing multiple early preantral follicles in one hanging drop of Peromyscus ovarian cell-conditioned medium. It is further revealed that the mechanical heterogeneity in the extracellular matrix of ovary is crucial for developing early preantral follicles to the antral stage and for the subsequent ovulation to release cumulus-oocyte complex. These findings may provide valuable guidance for furthering the technology of in vitro follicle culture to restore fertility in the clinic.

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A modified protocol for in vitro maturation of mouse oocytes from secondary preantral follicles

Cited by 8 publications

References 93 publications

High Levels Production of Recombinant Human Activin A—Effect upon <i>in Vivo</i> Follicle Stimulation

High Levels Production of Recombinant Human Activin A—Effect upon <i>in Vivo</i> Follicle Stimulation

In Vitro Culture of Early Secondary Preantral Follicles in Hanging Drop of Ovarian Cell-Conditioned Medium to Obtain MII Oocytes from Outbred Deer Mice

In vitro culture of ovarian follicles from Peromyscus

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A modified protocol for in vitro maturation of mouse oocytes from secondary preantral follicles

Cited by 8 publications

References 93 publications

High Levels Production of Recombinant Human Activin A—Effect upon &lt;i&gt;in Vivo&lt;/i&gt; Follicle Stimulation

High Levels Production of Recombinant Human Activin A—Effect upon &lt;i&gt;in Vivo&lt;/i&gt; Follicle Stimulation

In Vitro Culture of Early Secondary Preantral Follicles in Hanging Drop of Ovarian Cell-Conditioned Medium to Obtain MII Oocytes from Outbred Deer Mice

In vitro culture of ovarian follicles from Peromyscus

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High Levels Production of Recombinant Human Activin A—Effect upon <i>in Vivo</i> Follicle Stimulation

High Levels Production of Recombinant Human Activin A—Effect upon <i>in Vivo</i> Follicle Stimulation