A modified technique for small-bowel transplantation in the rat

Study of morbidity in orthotopic small intestine transplantation with Wistar rats: experimental study

“…Thus, there is great interest in the development of an adequate technique for intestinal transplantation in these animals, representing a model to be reproduced on a large-scale basis with low mortality. The disadvantage is that, since the rat is a small animal, microsurgical techniques are required; however, despite its complexity, the technique can be dominated by surgeons after appropriate training, as long as some microsurgery norms are followed (20,50) .…”

Difficulties, guidelines and review of developing an acute rejection model after rat intestinal transplantation

“…To date, HSBT has not been used to study early graft changes secondary to rejection [14]. In our experience, HSBT resulted in a valid and technically easier model, since long-term survival improved dramatically once we switched to HSBT at a time when most of the animals were already living at least 3 or 4 days after OSBT.…”

“…Although they mentioned some of the surgical complications and tips such as slowly washing the bowel lumen, no further details are provided in the paper, including the rat strains. Theirs was also a small sample, with only 8 survivors [14].…”

“…For these reasons, it is difficult to compare the various publications and to establish conclusions before starting as a novice in the field [4][5][6][8][9][10][11][12][13][14][15][16][17][18].…”

Small Bowel Transplantation: Past, Present and Future

“…How ever, it remains a more difficult procedure, and so has not been widely used. More recent ly, other variations have been introduced, in cluding combined small bowel and colon transplantation [34], the use of the renal pedi cle for the vascular connections [35] and the use of the superior mesenteric artery as the arterial connection [36], These models have not been shown to offer any specific advan tages over the previously described methods.…”