A modified two-component regulatory system is involved in temperature-dependent biosynthesis of the Pseudomonas syringae phytotoxin coronatine

Ullrich, Matthias S.; Peñaloza‐Vázquez, Alejandro; Bailey, Ana M.; Bender, Carol L.

doi:10.1128/jb.177.21.6160-6169.1995

Cited by 98 publications

(114 citation statements)

References 65 publications

(70 reference statements)

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“…These genes are located in a 32.8-kb plasmid-borne gene cluster, which also includes genes involved in coronatine biosynthesis (33,498). The corS, corR, and corP genes encode an unconventional two-component system consisting of a transmembrane histidine kinase, CorS, and two response regulators, CorR and CorP (498). CorP lacks a typical helix-turn-helix motif but possibly functions as a modulator of CorR or CorS activity (512).…”

Section: Production Of Phytotoxins By Pseudomonas Syringaementioning

confidence: 99%

“…The thermoregulation occurs at the level of transcription of the biosynthetic genes and is mediated by products of three regulatory genes, corS, corR, and corP. These genes are located in a 32.8-kb plasmid-borne gene cluster, which also includes genes involved in coronatine biosynthesis (33,498). The corS, corR, and corP genes encode an unconventional two-component system consisting of a transmembrane histidine kinase, CorS, and two response regulators, CorR and CorP (498).…”

Section: Production Of Phytotoxins By Pseudomonas Syringaementioning

confidence: 99%

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Detection of and Response to Signals Involved in Host-Microbe Interactions by Plant-Associated Bacteria

Brencic

Winans

2005

Microbiol Mol Biol Rev

339

208

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Diverse interactions between hosts and microbes are initiated by the detection of host-released chemical signals. Detection of these signals leads to altered patterns of gene expression that culminate in specific and adaptive changes in bacterial physiology that are required for these associations. This concept was first demonstrated for the members of the family Rhizobiaceae and was later found to apply to many other plant-associated bacteria as well as to microbes that colonize human and animal hosts. The family Rhizobiaceae includes various genera of rhizobia as well as species of Agrobacterium. Rhizobia are symbionts of legumes, which fix nitrogen within root nodules, while Agrobacterium tumefaciens is a pathogen that causes crown gall tumors on a wide variety of plants. The plant-released signals that are recognized by these bacteria are low-molecular-weight, diffusible molecules and are detected by the bacteria through specific receptor proteins. Similar phenomena are observed with other plant pathogens, including Pseudomonas syringae, Ralstonia solanacearum, and Erwinia spp., although here the signals and signal receptors are not as well defined. In some cases, nutritional conditions such as iron limitation or the lack of nitrogen sources seem to provide a significant cue. While much has been learned about the process of host detection over the past 20 years, our knowledge is far from being complete. The complex nature of the plant-microbe interactions makes it extremely challenging to gain a comprehensive picture of host detection in natural environments, and thus many signals and signal recognition systems remain to be described

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Section: Production Of Phytotoxins By Pseudomonas Syringaementioning

confidence: 99%

Section: Production Of Phytotoxins By Pseudomonas Syringaementioning

confidence: 99%

Detection of and Response to Signals Involved in Host-Microbe Interactions by Plant-Associated Bacteria

Brencic

Winans

2005

Microbiol Mol Biol Rev

339

208

View full text Add to dashboard Cite

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“…PG4180 produces COR predominantly at 18 u C, whereas there is no detectable COR production at 28 uC. Biosynthesis of COR in PG4180 is regulated at the transcriptional level by a modified two-component regulatory system composed of CorS, CorR and CorP (Ullrich et al, 1995), and by RpoN (s 54 ), an alternative sigma factor (Alarcó n- Chaidez et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…glycinea PG4180, which causes bacterial blight of soybeans (Budde et al, 1998;Palmer & Bender, 1993;Ullrich et al, 1995). Structurally, COR resembles a polyketide, and consists of two distinct moieties, coronafacic acid (CFA) and coronamic acid (CMA), which function as intermediates in the biosynthetic pathway to COR and are fused together by an amide bond (Mitchell et al, 1994;Parry et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Topological and deletion analysis of CorS, a Pseudomonas syringae sensor kinase

Smirnova

Ullrich

2004

Microbiology

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A modified two-component regulatory system consisting of two response regulators, CorR and CorP, and the histidine protein kinase CorS, regulates the thermoresponsive production of the phytotoxin coronatine (COR) in Pseudomonas syringae PG4180. COR is produced at the virulence-promoting temperature of 18 6C, but not at 28 6C, the optimal growth temperature of PG4180. Assuming that the highly hydrophobic N-terminus of CorS might be involved in temperature-signal perception, the membrane topology of CorS was determined using translational phoA and lacZ fusions, leading to a topological model for CorS with six transmembrane domains (TMDs). Interestingly, three PhoA fusions located downstream of the sixth TMD showed a thermoresponsive phenotype. Enzymic activity, immunoblot, and protease-sensitivity assays were performed to localize the CorS derivatives, to analyse the expression level of hybrid proteins and to examine the model. In-frame deletions of the last four, or all six TMDs gave rise to non-functional CorS. The results indicated that the transmembrane region is important for CorS to function as a temperature sensor, and that the membrane topology of CorS might be involved in signal perception.

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“…COR is produced in a temperature-dependent manner. Large amounts of the molecule are only synthesized at 18°C in vitro and in planta, whereas at 28°C toxin production is negligible [170][171][172]. The two gene clusters for COR biosynthesis are separated and controlled by the corSRP locus, encoding a modified two-component system [170,173,174].…”

Section: Thermosensing Through Phosphorylation Of Sensor Kinasesmentioning

confidence: 99%