A molecular epidemiological investigation of methicillin-susceptible Staphylococcus aureus causing bloodstream infections in Ireland, 2006–2017

Deasy, E.C.; Brennan, Gráinne I.; Tecklenborg, Sarah C; Umeh, Chioma; Coleman, David C.; Shore, Anna C.

doi:10.1007/s10096-019-03523-0

Cited by 10 publications

(16 citation statements)

References 31 publications

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“…Rather than showing clustering, a typical feature of MRSA, most of our methicillin-susceptible isolates displayed extensive genetic diversity. Our MSSA corresponded to major clones widespread in Poland and other European countries 19 , 49 , 51 – 54 . In contrast, different predominant clonal complexes were reported in Africa and Asia, indicating geographical variation 53 , 55 , 56 .…”

Section: Discussionmentioning

confidence: 70%

“…The epidemiology of S. aureus infections constantly changes, with novel clones emerging in various geographical regions 19 , 27 , 32 . This warrants continuous surveillance of staphylococcal isolates from different sources, especially from the oral cavity.…”

Section: Discussionmentioning

confidence: 99%

“…The predominant clonal complex in our material was CC45, with the most common spa type being spa- CC t015. CC45 are widely distributed among both nasal colonization and bloodstream infections strains in Europe 19 , 59 , and recent results suggest that the nasal isolates carry the potential to cause an invasive disease 59 . However, no reports on severe infections caused by oral CC45 strains have been published to date.…”

Section: Discussionmentioning

confidence: 99%

“…However, no reports on severe infections caused by oral CC45 strains have been published to date. Bonnet observed predominance of spa CC t015 among S. aureus associated with infective endocarditis 60 , and Deasy pointed to this spa type as an emerging etiological factor of bloodstream infections 19 . Other authors reported on infective endocarditis after dental extraction and treatment 61 , 62 .…”

Section: Discussionmentioning

confidence: 99%

“…According to Terpenning et al, the presence of S. aureus in saliva was a significant risk factor for aspiration pneumonia 18 . Also, a strong relationship was found between the isolation of S. aureus and the occurrence of other severe infections, such as bacteraemia and infective endocarditis 19 – 21 . As the majority of these infections are endogenous, the risk among S. aureus colonized patients is 11.5 times higher than in non-colonized persons 22 .…”

Section: Introductionmentioning

confidence: 99%

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Presence of egc-positive major clones ST 45, 30 and 22 among methicillin-resistant and methicillin-susceptible oral Staphylococcus aureus strains

Kwapisz

Garbacz

Kosecka-Strojek

et al. 2020

Sci Rep

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The oral cavity may comprise a significant reservoir for Staphylococcus aureus but the data on molecular epidemiology and clonal distribution of oral strains are really scarce. This study aimed to evaluate the clonal relatedness in S. aureus isolated from oral cavity and their relationship with carriage of virulence genes, and antimicrobial resistance profiles. A total of 139 oral S. aureus isolates were obtained from 2327 analysed oral samples of dental patients. Antimicrobial susceptibility testing was performed. Isolates were characterized using protein A gene (spa) typing, spa-CC clonal complexes, toxin genes and SCCmec typing for MRSA. High resistance rates for penicillin, tetracycline and gentamicin were detected, respectively 58.3%, 42.4%, and 35.2%. Twelve (8.6%) S. aureus isolates were identified as MRSA. All of MRSA isolates were mecA-positive and mecC-negative. SCCmec IV was the most common type (66.7%), which was typical for community-acquired MRSA (CA-MRSA). Overall, the enterotoxin gene cluster (egc) was the most frequent detected virulence factor (44.9%), both in MSSA and MRSA isolates. Presence of genes encoding for the enterotoxins (sea, seb, sec, seh, sek), exfoliative toxin A (eta), and toxic shock syndrome toxin-1 (tst) was also observed. Strains carrying lukS-PV/lukF-PV genes belonged to SCCmecV- spa type t437. The most prevalent spa types were t091, t015, t084, t002, t571, and t026 among all 57 identified. Spa types, including 3 new ones, grouped in 6 different spa-CC clonal complexes, with four major dominated; CC45, CC30, CC5, and CC15. This study demonstrated that both methicillin-susceptible and methicillin-resistant major European clones of S. aureus could be isolated from the oral cavity of dental patients, with the emergence of PVL-positive CA-MRSA strains. The oral cavity should be considered as a possible source of toxigenic egc-positive S. aureus strains, in terms of potential risk of cross-infection and dissemination to other body sites.

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