A molecular epidemiological study of human parainfluenza virus type 3 at a tertiary university hospital during 2013–2015 in Catalonia, Spain

Abstract

Human parainfluenza virus type 3 (HPIV-3) is one of the most common respiratory viruses particularly among young children and immunocompromised patients. The seasonality, prevalence and genetic diversity of HPIV-3 at a Spanish tertiary-hospital from 2013 to 2015 are reported. HPIV-3 infection was laboratory-confirmed in 102 patients (76%, under 5 years of age). Among <5 years-old patients, 9 (11.5%) were under any degree of immunosuppression, whereas this percentage was significantly higher (19; 79.2%) among patients older than 5 years. HPIV-3 was detected at varying levels, but mainly during spring and summer. All characterized HN/F sequences fell within C1b, C5 and in other two closely C3a-related groups. Furthermore, a new genetic lineage (C1c) was described. Genetic similarity and epidemiological data confirmed some nosocomial infections, highlighting the importance of the HPIV-3 surveillance, particularly in high-risk patients. This study provides valuable information on HPIV-3 diversity due to the scarce information in Europe.

Molecular Evolution of the Fusion Protein (F) Gene in Human Respirovirus 3

“…However, distribution of the distances in our study was similar to those in previous reports of the HRV3 F gene (Tsutsui et al, 2017), and also the HRV3 HN gene (Mao et al, 2012). This implied that the F and HN genes may be categorized into the same cluster and subcluster (Godoy et al, 2016;Košutić-Gulija et al, 2017). In addition, the genetic distances of the HRSV-A and HRSV-B F gene based on globally collected strains were also smaller than the present study, reported as 0.025 and 0.017, respectively (Kimura et al, 2016(Kimura et al, , 2017.…”

“…These findings may contribute to a better understanding of HRV3 infection in humans. Some molecular epidemiological studies of the HRV3 F gene have been reported (Godoy et al, 2016;Košutić-Gulija et al, 2017;Tsutsui et al, 2017). Godoy et al (2016) created a phylogenetic tree based on the partial sequence of the F gene (390 nt) by the neighbor-joining method.…”

“…Some molecular epidemiological studies of the HRV3 F gene have been reported (Godoy et al, 2016;Košutić-Gulija et al, 2017;Tsutsui et al, 2017). Godoy et al (2016) created a phylogenetic tree based on the partial sequence of the F gene (390 nt) by the neighbor-joining method. Moreover, Košutić-Gulija et al (2017) and Tsutsui et al (2017) performed a molecular epidemiologic study by the ML method using the full-length nucleotide sequences of the F gene from domestic HRV3 strains (Japanese and Croatian strains, respectively).…”

Human parainfluenza virus infection in severe acute respiratory infection cases in Beijing, 2014‐2016: A molecular epidemiological study

“…Several antigenic epitopes of the HN glycoprotein have been previously characterized. 12,13 Three amino acid substitutions in antigenic epitopes were found in this study, including K168R (16.67%), H295Y (100%), and I391V (100%). In particular, the K168R mutation locates very close to amino acids of significant importance to the integrity of distinct HN epitopes (residues 171), 14 and it has not been identified in Asia before.…”

Genetic Variability and Sequence Relatedness of Matrix Protein in Viruses of the Families Paramyxoviridae and Pneumoviridae

“…2). This is especially interesting as no changes that could distinguish different phylogenetic subgroups were present when we analyzed HPIV3 F and HN proteins [40], which are more diverse, and therefore used for phylogenetic classification [41, 42]. Also, in the case of HPIV3, most of the amino acid changes in glycoproteins were restricted to cytoplasmic and transmembrane regions [40], which are regions thought to be important for interaction with the M proteins [43].…”