A molecular genetics of Drosera spatulata complex by using of RAPD analysis

Abstract

ABSTRACT. RAPD analysis was carried out using to infer the characteristics of RAPD generated DNA fragment and to investigate phylogenetic relationships in three species of D. spatulata complex with different types of genome compositions and ploidy levels. A total of 60 decamer random sequences were initially screened to select using analysis primers. The result of RAPD analysis in the present study showed that D. rotundifolia and D. tokaiensis clustered more closely than D. spatulata. Thus, DNA fragments obtained from RAPD primer might have a priority to amplify certain DNA from the haploid genome set with larger chromosome size than that of smaller size in Drosera spatulata complex species.

Breeding and Cytogenetic Characterizations of New Hexaploid <i>Drosera</i> Strains Colchicine-Induced from Triploid Hybrid of <i>D. rotundifolia</i> and <i>D. spatulata</i>

“…4, Table 3). Our previous paper demonstrated that the hexaploid D. tokaiensis had amphidiploidal hybrid origin between D. spatulata and D. rotundifolia (Hoshi et al 2010). Since the artificial-crossing triploid hybrid was made from D. spatulata and D. rotundifolia, the genome constitution of the colchicine-induced hexaploid is the same as D. Hoshi and Kondo 1998).…”

“…D. rotundifolia is the diploid species with middle size chromosomes (2n=2x=20M), while D. spatulata is the tetraploid species with small size chromosomes (2n=4x= 40S). In contrast, D. tokaiensis is the amphidiploidal hexaploid species (2n=6x= 20M+40S) with a hybrid origin between D. spatulata and D. rotundifolia (Hoshi et al 2010). Triploid hybrids from D. spatulata and D. rotundifolia have been produced by artificial crossing and are sterile.…”

“…Carnivorous plants are extraordinary plants because of their highly specialized morphological characters for insect trapping (Rivadavia et al 2003, Hoshi et al 2010). The family Droseraceae, which is one of the representative carnivorous plants, consists of three genera: Aldrovanda, Dionaea and Drosera (Rivadavia et al 2003, Shirakawa et al 2011.…”

Random Amplified Polymorphic DNA (RAPD) Analysis of Three Japanese <i>Aster</i> Species

“…This indicates that the species with the L-type chromosomes shares many fragments that the S-type chromosomes lack. Current molecular work on Drosera suggested that the DNA fragments obtained by RAPD primers were preferentially amplified from the species containing a common genome consisting of large chromosomes (Hoshi et al 2010). This amplifying preference led us to predict that RAPD-generated DNA fragments possessed many of the DNA sequences related to chromosome size changes.…”

“…The RAPD technique could identify genotypes directly and help to mitigate complications arising from earlier cytological and morphological studies (Das 2008). However, in recent molecular work on angiosperms, RAPD generated-DNA sequences were more repetitive in the genome (Koo et al 2005), and were obtained preferrentially from a genome with karyotypes consisting of large chromosomes (Hoshi et al 2010).…”