A molecular map of the chicken major histocompatibility complex: the class II beta genes are closely linked to the class I genes and the nucleolar organizer.

Guillemot, François; Billault, Alain; Pourquié, Olivier; Béhar, Ghislaine; Chaussé, Anne-Marie; Zoorob, Rima; Kreibich, Gert; Auffray, Charles

doi:10.1002/j.1460-2075.1988.tb03132.x

Cited by 250 publications

(144 citation statements)

References 50 publications

Supporting

Mentioning

138

Contrasting

Order By: Relevance

“…At the start of this work, it had been reported that there were four classical class I genes in the chicken MHC (B12 haplotype), but studies of two haplotypes (B12 and B19) had reported cDNA clones for only one gene (2,15).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Peptide motifs of the single dominantly expressed class I molecule explain the striking MHC-determined response to Rous sarcoma virus in chickens

Wallny

Ávila

Hunt

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

172

243

View full text Add to dashboard Cite

Compared with the MHC of typical mammals, the chicken MHC is smaller and simpler, with only two class I genes found in the B12 haplotype. We make five points to show that there is a singledominantly expressed class I molecule that can have a strong effect on MHC function. First, we find only one cDNA for two MHC haplotypes (B14 and B15) and cDNAs corresponding to two genes for the other six (B2, B4, B6, B12, B19, and B21). Second, we find, for the B4, B12, and B15 haplotypes, that one cDNA is at least 10-fold more abundant than the other. Third, we use 2D gel electrophoresis of class I molecules from pulse-labeled cells to show that there is only one heavy chain spot for the B4 and B15 haplotypes, and one major spot for the B12 haplotype. Fourth, we determine the peptide motifs for B4, B12, and B15 cells in detail, including pool sequences and individual peptides, and show that the motifs are consistent with the peptides binding to models of the class I molecule encoded by the abundant cDNA. Finally, having shown for three haplotypes that there is a single dominantly expressed class I molecule at the level of RNA, protein, and antigenic peptide, we show that the motifs can explain the striking MHC-determined resistance and susceptibility to Rous sarcoma virus. These results are consistent with the concept of a ''minimal essential MHC'' for chickens, in strong contrast to typical mammals.antigen presentation ͉ avian ͉ essential ͉ evolution ͉ minimal

show abstract

Section: Resultsmentioning

confidence: 99%

“…chicken MHC is simpler and smaller, with the genes arranged differently (1)(2)(3)(4)(5). Moreover, the chicken MHC can determine decisive resistance and susceptibility to several infectious pathogens, as well as response to both live and killed commercial vaccines (6)(7)(8)(9).…”

mentioning

confidence: 99%

Peptide motifs of the single dominantly expressed class I molecule explain the striking MHC-determined response to Rous sarcoma virus in chickens

Wallny

Ávila

Hunt

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

172

243

View full text Add to dashboard Cite

show abstract

“…HA has the advantage of speed and ease. Besides the MHC complex, chickens also have an Rfp-Y-system (Guillemot et al, 1988;Miller et al, 1994). However, identi¢cation of Rfp-Y (MHC-like) haplotypes seems of minor importance (Pharr et al, 1996(Pharr et al, , 1997.…”

Section: Introductionmentioning

confidence: 99%

Serological Screening for MHC (B)-Polymorphism in Indigenous Chickens

Baelmans

Parmentier

Nieuwland

et al. 2005

Tropical Animal Health and Production

View full text Add to dashboard Cite

As part of a series of studies to characterize innate and speci¢c immune responses of indigenous chicken lines, birds from Bolivia and India were screened serologically for MHC class IV (BG) polymorphism by direct haemagglutination using haplotype-speci¢c antisera (B2, B4, B12, B13, B14, B15, B19, B21). The sample consisted of 95 Bolivian indigenous chickens and 119 hens from the four most common North Indian`back-yard' chicken lines: Yellow Aseel (AP), Kadaknath (KN), frizzled typed (Ff^) and naked neck (NN). Of all chickens tested, the majority were haplotyped as B2, B15, B19 and B21. Of the Bolivian chickens, 89.5% could be haplotyped: 54.9% were homozygous (including 43.3% B15), and 34.6% were heterozygous (including 15.7% B15). B2-like haplotypes were not found among the Bolivian hens, and only 3.2% of these birds showed homozygous B21-like proteins. Of the Indian hens, MHC (BG)-like proteins could be detected in 60.0% of the AP birds, 6.7% of the KN birds; 40.0% of the Ff^birds; and 10.3% of the NN birds. In these lines, a total of 40.1% (AP), 6.7% (KN), 30.1% (Ff^) and 10.3% (NN) were homozygous for the B-haplotype. Only in the AP line (19.9%), and the Ff^line (9.9%) were heterozygous B-haplotypes types found. The B2 haplotype was found in all Indian chicken lines. Most Indian birds have completely unknown haplotypes, indicating a potentially interesting genetic pool. Subgrouping the Bolivian and Indian indigenous hens into monomorphic BG populations revealed individual di¡erences based on the B-types.

show abstract

“…Among the genetic loci potentially involved in the immune response, the chicken MHC can determine decisive resistance and susceptibility to several infectious pathogens, as well as the response to both live and killed commercial vaccines (1)(2)(3)(4). Compared with typical mammals such as humans and mice, the chicken MHC is simpler, smaller, and rearranged (5)(6)(7)(8). Based on these data, we developed the concept of a "minimal essential MHC" to give a molecular basis to the striking functional properties of the chicken MHC (7)(8)(9).…”

mentioning

confidence: 99%

Different Evolutionary Histories of the Two Classical Class I Genes BF1 and BF2 Illustrate Drift and Selection within the Stable MHC Haplotypes of Chickens

Shaw

Powell

Marston

et al. 2007

The Journal of Immunology

108

View full text Add to dashboard Cite

Compared with the MHC of typical mammals, the chicken MHC (BF/BL region) of the B12 haplotype is smaller, simpler, and rearranged, with two classical class I genes of which only one is highly expressed. In this study, we describe the development of long-distance PCR to amplify some or all of each class I gene separately, allowing us to make the following points. First, six other haplotypes have the same genomic organization as B12, with a poorly expressed (minor) BF1 gene between DMB2 and TAP2 and a well-expressed (major) BF2 gene between TAP2 and C4. Second, the expression of the BF1 gene is crippled in three different ways in these haplotypes: enhancer A deletion (B12, B19), enhancer A divergence and transcription start site deletion (B2, B4, B21), and insertion/rearrangement leading to pseudogenes (B14, B15). Third, the three kinds of alterations in the BF1 gene correspond to dendrograms of the BF1 and poorly expressed class II B (BLB1) genes reflecting mostly neutral changes, while the dendrograms of the BF2 and well-expressed class II (BLB2) genes each have completely different topologies reflecting selection. The common pattern for the poorly expressed genes reflects the fact the BF/BL region undergoes little recombination and allows us to propose a pattern of descent for these chicken MHC haplotypes from a common ancestor. Taken together, these data explain how stable MHC haplotypes predominantly express a single class I molecule, which in turn leads to striking associations of the chicken MHC with resistance to infectious pathogens and response to vaccines.

show abstract

A molecular map of the chicken major histocompatibility complex: the class II beta genes are closely linked to the class I genes and the nucleolar organizer.

Abstract: A molecular map of the chicken major histocompatibility complex: the class 11 genes are closely linked to the class I genes and the nucleolar organizer

Cited by 250 publications

References 50 publications

Peptide motifs of the single dominantly expressed class I molecule explain the striking MHC-determined response to Rous sarcoma virus in chickens

Peptide motifs of the single dominantly expressed class I molecule explain the striking MHC-determined response to Rous sarcoma virus in chickens

Serological Screening for MHC (B)-Polymorphism in Indigenous Chickens

Different Evolutionary Histories of the Two Classical Class I Genes BF1 and BF2 Illustrate Drift and Selection within the Stable MHC Haplotypes of Chickens

Contact Info

Product

Resources

About