Serological Screening for MHC (B)-Polymorphism in Indigenous Chickens

Abstract

As part of a series of studies to characterize innate and speci¢c immune responses of indigenous chicken lines, birds from Bolivia and India were screened serologically for MHC class IV (BG) polymorphism by direct haemagglutination using haplotype-speci¢c antisera (B2, B4, B12, B13, B14, B15, B19, B21). The sample consisted of 95 Bolivian indigenous chickens and 119 hens from the four most common North Indian`back-yard' chicken lines: Yellow Aseel (AP), Kadaknath (KN), frizzled typed (Ff^) and naked neck (NN). Of all chickens tested, the majority were haplotyped as B2, B15, B19 and B21. Of the Bolivian chickens, 89.5% could be haplotyped: 54.9% were homozygous (including 43.3% B15), and 34.6% were heterozygous (including 15.7% B15). B2-like haplotypes were not found among the Bolivian hens, and only 3.2% of these birds showed homozygous B21-like proteins. Of the Indian hens, MHC (BG)-like proteins could be detected in 60.0% of the AP birds, 6.7% of the KN birds; 40.0% of the Ff^birds; and 10.3% of the NN birds. In these lines, a total of 40.1% (AP), 6.7% (KN), 30.1% (Ff^) and 10.3% (NN) were homozygous for the B-haplotype. Only in the AP line (19.9%), and the Ff^line (9.9%) were heterozygous B-haplotypes types found. The B2 haplotype was found in all Indian chicken lines. Most Indian birds have completely unknown haplotypes, indicating a potentially interesting genetic pool. Subgrouping the Bolivian and Indian indigenous hens into monomorphic BG populations revealed individual di¡erences based on the B-types.

Illumina Next Generation Sequencing for the Analysis of Eimeria Populations in Commercial Broilers and Indigenous Chickens

“…The first, the Cobb400, is a commercial broiler line used widely in India that is descended from Cobb500 and Cobb100 hybrids, the latter of which had previously been acclimatized within India ( 23 ). The second was the indigenous Indian Kadaknath chicken, a breed prized for its “black” meat, reported to be resistant to some infectious diseases ( 24 , 25 ), and to present immune parameters distinct from those of modern commercial broiler chickens ( 26 , 27 ).…”

Microbial diversity and community composition of caecal microbiota in commercial and indigenous Indian chickens determined using 16s rDNA amplicon sequencing

“…For example, 17 breeds of Indian origin are currently registered with the National Bureau of Animal Genetic Resources (NBAGR) at the Indian Council of Agricultural Research (ICAR) ( http://www.nbagr.res.in/regchi.html ). Among these breeds examples such as Aseel and Kadaknath have been associated with improved egg, meat and welfare traits such as reduced feather pecking [ 20 , 21 ], resistance to infectious disease [ 22 , 23 ] and immune parameters distinct from those of modern commercial chickens [ 24 , 25 ]. Variation in immunity-related pathways can influence microbiome structure in humans [ 26 ] and is also likely to affect chicken microbiomes [ 27 ].…”

Genetic diversity at major histocompatibility complex and its effect on production and immune traits in indigenous chicken breeds of India

“…Various studies have used genotyping of the LEI0258 marker to assess the genetic diversity at MHC (Nikbakht et al, 2013;Han et al, 2013;Guangxin et al, 2014). In a study, Baelmans et al (2005) detected completely unknown haplotypes using serological method in four lines of indigenous chickens of India, thus reflecting the potentially interesting genetic pool at MHC. Therefore, the present study was carried out with the objective of investigating the genetic variability at MHC in indigenous and WLH breeds of chicken and to study the association of alleles of LEI0258 on growth, production, immunity and survivability traits in indigenous chicken breeds in comparison with the improved WLH breed.…”