A molecular protocol for Early Sex Discrimination (ESD) in Actinidia spp

Mori, Gloria De; Testolin, R.; Cipriáni, G.

doi:10.3233/jbr-211530

Cited by 5 publications

(3 citation statements)

References 28 publications

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“…The sex-discriminating marker in kiwifruit breeding is an essential tool that helps shorten breeding cycles and promote the development of improved cultivars [41]. Previously, we developed a CAPS marker discriminating female and male A. arguta accessions [18], but it requires restriction enzyme treatment.…”

Section: Discussionmentioning

confidence: 99%

A MADS-Box Gene-Based InDel Marker Discriminating Sex in Actinidia arguta

Oh,

Kim,

Kim

et al. 2023

Horticulturae

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Gene-based markers are valuable tools in breeding programs due to their direct linkage to traits of interest. In dioecious plants, such as kiwifruit (Actinidia spp.), sex-discriminating markers can shorten the breeding cycle by enabling the selection of preferred sexes at the juvenile stage. To develop a gene-based sex-discriminating marker, resequencing was conducted on female and male A. arguta accessions, and insertion and deletion (InDel) variations within sex-related genes were explored. A total of 203,116 InDels were detected between female and male A. arguta accessions, and 118,865 InDels were heterozygous between the two accessions. Sequence similarity between thirty-seven sex-related genes from seven dioecious species and the kiwifruit reference genome was investigated, revealing that ten genes exhibited similarities ranging from 59 to 79%. Among the 118,865 InDels, seven InDels were located on four sex-related genes encoding agamous-like MADS-box genes and hypothetical proteins. A 20 bp insertion in male A. arguta located in the agamous-like MADS-box gene was converted into an InDel marker, which clearly discriminates female and male A. arguta accessions and the interspecific hybrid cultivar. The InDel marker was designated CBk25id01 and produced approximately 350 bp amplicon only in the male A. arguta. The CBk25id01 linked to the agamous-like MADS-box gene involved in floral organ development may help understand sex differentiation and accelerate the breeding of kiwifruits.

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Section: Discussionmentioning

confidence: 99%

A MADS-Box Gene-Based InDel Marker Discriminating Sex in Actinidia arguta

Oh,

Kim,

Kim

et al. 2023

Horticulturae

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“…In male genotypes, all three markers produce an amplicon each, while female genotypes produce an amplicon only for Ankyrin . Such simple signals of presence/absence discriminate the gender in different species at any ploidy level (De Mori et al., 2022).…”

Section: Markers In Selected Species Of Fruit Cropmentioning

confidence: 99%

SSR‐based DNA fingerprinting of fruit crops

et al. 2023

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The DNA fingerprinting of fruit crops, based on DNA microsatellite markers that are considered to be the key markers for the molecular analysis of germplasm collections, is reviewed. Simple sequence repeats (SSRs) remain the markers of choice for fingerprinting in humans, animals, plants, and other living organisms. This review, that considers 44 fruit species, provides a set of markers that are suitable for profiling accessions and that should make the databases produced in different laboratories more comparable. Every effort has been made to select SSR markers that are robust and easily scorable considering that such analyses are sometimes used in legal cases. The review first describes the basic protocols, procedures, and methods of data analyses; it then describes the fingerprinting of individual species or groups of species, providing a set of SSR markers and appropriate guidance based on the revised literature and on the authors' experience.

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“…In fact, breeders are generally more interested in selecting productive individuals, and eliminating males saves considerable economic resources. In Actinidia, a simple-to-use molecular marker has recently been developed which allows for this type of selection on seedlings [50]. Other sex-linked molecular markers have been developed for various fruit crops such as papaya (SCART1, SCART12, and SCARW11) [51], ginkgo (GBA and GBB) [52], kaki (DlSx-AF4S) [53], mulberry (MBS markers) [54], and pistachio (SNP-PIS-167992 and P-ATL-91951-565) [55].…”

Section: Phenological Traits Related To Fruit Productionmentioning

confidence: 99%

Marker-Assisted Selection in Breeding for Fruit Trait Improvement: A Review

Mori

Cipriáni

2023

IJMS

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Breeding fruit species is time-consuming and expensive. With few exceptions, trees are likely the worst species to work with in terms of genetics and breeding. Most are characterized by large trees, long juvenile periods, and intensive agricultural practice, and environmental variability plays an important role in the heritability evaluations of every single important trait. Although vegetative propagation allows for the production of a significant number of clonal replicates for the evaluation of environmental effects and genotype × environment interactions, the spaces required for plant cultivation and the intensity of work necessary for phenotypic surveys slow down the work of researchers. Fruit breeders are very often interested in fruit traits: size, weight, sugar and acid content, ripening time, fruit storability, and post-harvest practices, among other traits relevant to each individual species. The translation of trait loci and whole-genome sequences into diagnostic genetic markers that are effective and affordable for use by breeders, who must choose genetically superior parents and subsequently choose genetically superior individuals among their progeny, is one of the most difficult tasks still facing tree fruit geneticists. The availability of updated sequencing techniques and powerful software tools offered the opportunity to mine tens of fruit genomes to find out sequence variants potentially useful as molecular markers. This review is devoted to analysing what has been the role of molecular markers in assisting breeders in selection processes, with an emphasis on the fruit traits of the most important fruit crops for which examples of trustworthy molecular markers have been developed, such as the MDo.chr9.4 marker for red skin colour in apples, the CCD4-based marker CPRFC1, and LG3_13.146 marker for flesh colour in peaches, papayas, and cherries, respectively.

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A molecular protocol for Early Sex Discrimination (ESD) in Actinidia spp

Cited by 5 publications

References 28 publications

A MADS-Box Gene-Based InDel Marker Discriminating Sex in Actinidia arguta

A MADS-Box Gene-Based InDel Marker Discriminating Sex in Actinidia arguta

SSR‐based DNA fingerprinting of fruit crops

Marker-Assisted Selection in Breeding for Fruit Trait Improvement: A Review

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