A molecularly imprinted photonic polymer sensor with high selectivity for tetracyclines analysis in food

Abstract

A molecularly imprinted photonic polymer (MIPP) sensor for respective detection of tetracycline, oxytetracycline and chlortetracycline is developed based on the combination of a colloidal crystal templating method and a molecular imprinting technique. Colloidal crystal templates are prepared from monodisperse polystyrene colloids. The molecularly imprinted polymer, which is embodied in the colloidal crystal templates, is synthesized with acrylic acid and acrylamide as monomers, N,N'-methylene bisacrylamide as a cross-linker and tetracyclines (TCs) as imprinting template molecules. After removal of the colloidal crystal template and the molecularly imprinted template, the resulted MIPP consists of a three-dimensional, highly ordered and interconnected macroporous array with a thin hydrogel wall, where nanocavities complementary to analytes in shape and binding sites are distributed. The response of MIPP to TCs stimulants in aqueous solution is detected through a readable Bragg diffraction red-shift, which is due to the lattice change of MIPP structures responding to their rebinding to the target TCs molecules. A linear relationship was found between the Δλ and the concentration of TCs in the range from 0.04 μM to 0.24 μM. With this sensory system, direct and selective detection of TCs has been achieved without using label techniques and expensive instruments. The developed method has been applied successfully to detect tetracycline in milk and honey samples.

Hydrophilic–Hydrophobic Patterned Molecularly Imprinted Photonic Crystal Sensors for High‐Sensitive Colorimetric Detection of Tetracycline

“…[ 14 ] When a droplet of TC solution was dripped on the MIP-PC sensor, as the water evaporation, the TC molecules in the droplet were enriched to the hydrophilic MIP-PC dot by wettability difference and selectively recognized by the MIP by forming hydrogen bond ( Figure 2 a). The spectra of MIP-PC dot during the enrichment gradually shifts (Figure 2 b), so a suitable time to measure the spectra with the largest peak shift need to be found.…”

“…This result is one order of magnitude lower than that of traditional MIP-PC fi lm. [ 14 ] Moreover, the relationship between the size of detection area and the detection range was systematically investigated, and it has been concluded as a fan-shaped standard color card. This conclusion is of great importance for designing the sensors with suitable size of detection area and detection range in practical applications.…”

Molecular imprinted photonic crystal for sensing of biomolecules

“…Using the similar method, the MIPCS with inverse opal structure for sensing of theophylline and atrazine were also reported by the same group [32]. Wang also reported a MIPCB with high selectivity for tetracyclines analysis in food using polystyrene (PS) colloidal crystal as sacrificial templates [22]. In this report, AAm and AAc were both used as monomers, BIS was used as crosslinker.…”

“…Several MIPCB have been developed for sensing the biomolecules, such as atropine, cholic acid [29], diethylstilbestrol [31], antibiotics [22], imidacloprid [5], theophylline [32], and so on.…”

Molecularly imprinted polymers: possible use for isolation of biosynthetic antibiotics

“…Most of these sensors are electrochemical (potentiomet ric, 26-30,37,38,47,58 ammetric, 23-25,36,51,59,78,85,86,110,120,121 and voltammetric 31,87,116,125 ). However, two optical (pho ton32 and surface plasmon resonance 119 ) and piesoelectric sensors105 have also been reported.…”