A molecularly imprinted receptor for separation of testosterone and epitestosterone, based on a steroidal cross-linker

Preparation and evaluation of a molecularly imprinted polymer for the selective recognition of testosterone—application to molecularly imprinted sorbent assays

“…This prompted us to seek conditions for the optimization of testosterone‐specific MIPs for high‐binding affinities. Literature data showed that testosterone–MIPs have been synthesized in chloroform (Cheong et al ., ; Noss et al ., ; Gavrilovic et al ., ) and acetonitrile (Rachkov et al ., ; Qiu et al ., ; Long et al ., ). Rachkov et al .…”

“…This prompted us to seek conditions for the optimization of testosterone-specific MIPs for high-binding affinities. Literature data showed that testosterone-MIPs have been synthesized in chloroform (Cheong et al, 1997Noss et al, 2008;Gavrilovic et al, 2011) and acetonitrile Qiu et al, 2010;Long et al, 2011). Rachkov et al did the synthesis in both solvents in an oven at 40 C and reported that polymers prepared in acetonitrile presented a higher association constant but similar number of high-affinity binding sites (K a = 1.05 Â 10 4 M À1 and N = 1.2 mmol/g) than polymers prepared in chloroform (K a = 0.094 Â 10 4 M À1 and N = 1.6 mmol/g), as deduced from batch rebinding studies.…”

“…TFMAA is more acidic than MAA because of the electron-withdrawing effect of the trifluoromethyl group, and it has been shown that TFMAA-based polymers exhibit higher affinity and selectivity than MAA-based polymers for nicotine (Matsui et al, 1997) and cinchona alkaloids (Matsui et al, 2000). TFMAA is also a stronger hydrogen bond donor than MAA, but the potential benefit from this is canceled out by the fact that it also interacts more strongly with ethyleneglycol dimethacrylate (EDMA) than does MAA, decreasing the amount effectively available for template complexation (Yilmaz et al, 1999;Gavrilovic et al, 2011). Indeed, it has been shown that using TFMAA in combination with divinylbenzene (DVB) rather than EDMA led to a twofold increase in the imprinting factor for testosterone (Gavrilovic et al, 2011).…”

“…TFMAA is also a stronger hydrogen bond donor than MAA, but the potential benefit from this is canceled out by the fact that it also interacts more strongly with ethyleneglycol dimethacrylate (EDMA) than does MAA, decreasing the amount effectively available for template complexation (Yilmaz et al, 1999;Gavrilovic et al, 2011). Indeed, it has been shown that using TFMAA in combination with divinylbenzene (DVB) rather than EDMA led to a twofold increase in the imprinting factor for testosterone (Gavrilovic et al, 2011).…”

Designing and preparation of cytisine alkaloid surface-imprinted material and its molecular recognition characteristics

“…A c c e p t e d M a n u s c r i p t 7 For SAS-PGMA/SiO 2 particles, their FTIR was determined with KBr pellet method to confirm their chemical structure, their morphology was observed with SEM, and the bonding rate (BR, mol%) of SAS, namely the extent (mol%) of the ring-opening reaction of the epoxy groups of the grafted PGMA, was measured by weighing method and it was 70%. Besides, the zeta potentials of SAS-PGMA/SiO 2 particles at different pH values were determined with a Zeta potential analyzer, and their zeta curve was figured.…”