A monoclonal antibody and functional study of malignant T cells of a patient with suppressor-T-cell lymphoma

Raziuddin, Syed; Hussain, Nasr; Latif, Abdul

doi:10.1016/0090-1229(87)90037-7

Cited by 11 publications

(5 citation statements)

References 7 publications

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“…In the last 2 years, eight out of all the lymphoma cases studied in this laboratory were found to have unusual phenotypes of T cell lymphoma. A case of suppressor T cell lymphoma (OKT3+, OKT11-, OKT8+) in a Saudi woman was reported earlier [11]. In this report, we describe the clinical and immunological features of seven other patients with immunologically confirmed peripheral T cell lymphomas.…”

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confidence: 81%

“…Monocytes were identified by OKMI monoclonal antibodies. The monoclonal antibodies were purchased from Ortho Diagnostics, and were used according to the supplier's instructions in an indirect immunofluorescence technique using a Nikon optiphot microscope [11]. LNC were stimulated with phytohaemagglutinin (PHA, 2/ig/ml), concanavalin A (Con A, 10//g/ml), and pokeweed mitogen (PWM, 10 /Ig/ml) for 48 h at 37^ with 5% CO2 in complete RPMl 1640 medium.…”

Section: Methodsmentioning

confidence: 99%

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Peripheral T Cell Lymphomas: An Immunological Study of Seven Unusual Cases

et al. 1988

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A multiparameter study of malignant lymph node cells and peripheral blood lymphocytes of seven patients with peripheral T cell lymphoma is presented. The results of monoclonal marker studies showed three cases of helper-suppressor T cell lymphoma (OKT4+, OKT8+), one case of suppressor T cell lymphoma (OKT8+), and three cases of helper T cell lymphoma (OKT4+). Immunophenotypic heterogeneity of neoplastic T cells with expression of pan-T antigens, OKT3+, and OKT11+ (erythrocyte rosetting+) was observed in most patients. Six of the seven cases tested showed Ia and DR antigens. No relationship was detected between patterns of reactivity with T cell reagents and histological types. When tested, the in-vitro malignant T cells of five patients proliferated in response to concanavalin A (Con A), but had poor response to phytohaemagglutinin. The interleukin 2 receptors showed maximum expression on Con A-activated T cells of five patients, and phytohaemagglutinin-activated T cells of one patient. The neoplastic T cells (OKT4+, OKT8+) of one patient studied had suppressor activity for IgG and IgA, and helper activity for IgM synthesis on pokeweed mitogen-induced normal B cell differentiations.

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confidence: 81%

Section: Methodsmentioning

confidence: 99%

Peripheral T Cell Lymphomas: An Immunological Study of Seven Unusual Cases

et al. 1988

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“…Lymph node cell (LNC) suspensions obtained by gently scraping the tissue with a scalpel were purified on Histopaque‐1077 (Sigma Chemical Co., St Louis, Mo.) density gradient centrifugation as desribed previously ( Raziuddin et al , 1987 , 1994b). LNC were suspended in RPMI‐1640 medium containing 10% fetal calf serum, 100 U/ml penicillin and 100 μg/ml streptomycin (Flow Laboratories, Finland).…”

Section: Methodsmentioning

confidence: 99%

“…Non‐Hodgkin's lymphomas (NHL), the most common malignancy worldwide‐constitute a heterogenous group of lymphoid tumours, the majority of which are of the B‐cell type ( Knowles & Halper, 1982; Weiss et al , 1985 ; Foon & Todd, 1986; Raziuddin et al , 1987 , 1991). Of B‐cell and T‐cell neoplasms (NHL disease), B‐cell NHL have been investigated most vigorously.…”

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Regulation of interleukin‐4 production and cytokine‐induced growth potential in peripheral T‐cell non‐Hodgkin's lymphomas

Raziuddin

Sheikha²,

Abu-Eshy

et al. 1998

Br J Haematol

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The malignant cells in tumour tissues produce cytokines/growth factors that may influence tumour growth, tumour immunogenicity and host immune response. We demonstrate that lymph node cell (LNC) purified neoplastic T cells from CD4+ peripheral T‐cell lymphoma (CD4+ PTCL) and CD8+ PTCL spontaneously, and after stimulation with anti‐CD3, secreted high amounts of interleukin‐4 (IL‐4) as compared to LNC‐purified CD4+ and CD8+ non‐malignant T cells. Furthermore, IL‐4 was observed to be the most potent cytokine that induced in vitro proliferation and growth of the malignant T cells. Moreover, malignant T‐cell‐derived IL‐4 secretion was augmented by exogeneous recombinant human interferon‐gamma (IFN‐γ) and was profoundly inhibited by IL‐2. Because IL‐4 was shown to be a locally active cytokine with a wide range of immunoregulatory properties, regulation of IL‐4 production by IFN‐γ and IL‐2 in malignant T cells may be one of the important parameters to be assessed in the design of anticancer‐specific immunotherapy. In summary, we report that malignant T cells produce IL‐4, a type 2 cytokine (Th2 cell response) that acts as a growth factor and which may play a critical role in PTCL disease mechanism.

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Activated CD4-positive T-lymphocytes and impaired cell-mediated immunity in patients with carcinoma of the urinary bladder with schistosomiasis

Raziuddin

Shetty

Aliyu

et al. 1990

Cancer

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Patients with schistosomiasis of the urinary bladder (SB) associated with carcinoma of the bladder (SCB) or carcinoma of the prostate (SCP) have a variety of immunologic abnormalities, including the presence of HLA‐DR+ and interleukin‐2 receptor‐positive (IL‐2R+) T‐lymphocytes in circulating blood. This study demonstrated that, the HLA‐DR+ and IL‐2R+ antigens are selectively expressed on majority of the CD4+ T‐lymphocytes of patients with SCB, whereas, these antigens are expressed almost equally on both CD4+ and CD8+ T‐lymphocytes of patients with SB and SCP. Expressions of HLA‐DR+ and IL‐2R+ antigens in CD4+ T‐lymphocytes, and a depressed response of this T‐cell subset to phytohemagglutinin and Concanavalin A stimulations seems to be the characteristic feature of these patients with SCB. In addition, the autologous mixed lymphocyte reaction (AMLR) and allogenic mixed lymphocyte reaction (MLR) was depressed in patients with SCB. However, patients with SCP demonstrated a normal MLR, even though the AMLR was highly depressed. The immunoregulatory role of the HLA‐DR+, IL‐2R+, CD4+ helper/inducer T‐lymphocytes, and the AMLR and MLR abnormalities we have identified in patients with SCB may be important and could play a role in the pathobiology of these diseases in humans.

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A monoclonal antibody and functional study of malignant T cells of a patient with suppressor-T-cell lymphoma

Cited by 11 publications

References 7 publications

Peripheral T Cell Lymphomas: An Immunological Study of Seven Unusual Cases

Peripheral T Cell Lymphomas: An Immunological Study of Seven Unusual Cases

Regulation of interleukin‐4 production and cytokine‐induced growth potential in peripheral T‐cell non‐Hodgkin's lymphomas

Activated CD4-positive T-lymphocytes and impaired cell-mediated immunity in patients with carcinoma of the urinary bladder with schistosomiasis

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