A monoclonal antibody identifies a novel epitope surrounding a subpopulation of the mammalian central neurons

Watanabe, Eiji; Fujita, Shinobu C.; Murakami, Fujio; Hayashi, Muneaki; Matsumura, Masahiko

doi:10.1016/0306-4522(89)90137-1

Cited by 57 publications

(54 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other MAbs (Fujita et al, 1989: Watanabe et a]., 1989) have detected PG on the surface of selected neurons, and results that are not included here showed C4-S04 on the surface of relatively infrequent cortical neurons. The relation of cells detected with these methods and cells visualized with VC1.1, W A , and anti-C6-S04 remains unknown.…”

Section: Variability Among Neurons In Glycoconjugate Contentmentioning

confidence: 68%

“…Ultrastructural immunostaining revealed PG in cytosol of some, but not other, neurons and astrocytes in adult rats (Aquino et al, 1984) and detected mostly c 4 -s o 4 in adult and C6-SO4 in developing rat brain (Flaccus et al, 1991). Immunostaining at the light microscopic level revealed glycosaminoglycans surrounding subsets of neurons in brain (Fujita et a]., 1989; Watanabe et al, 1989) and localized C6-S04 at the surface of a large population of neurons in cerebral cortex (Kosaka et al, 1990).…”

Section: Imentioning

confidence: 93%

See 1 more Smart Citation

Differentiation of glycoconjugates localized to sensory terminals and selected sites in brain

Spicer¹,

Naegele²,

Schulte³

1996

J. Comp. Neurol.

View full text Add to dashboard Cite

Distribution of complex carbohydrates in the peripheral and central nervous systems was investigated cytochemically with a lectin that binds specifically to terminal alpha GalNAc and with monoclonal antibodies against carbohydrate epitopes, including glucuronic acid 3-SO4 and chondroitins 6-SO4 and 4-SO4. Comparative staining with these methods differentiated and partially characterized several glycoconjugates in various sites and allowed a comparison of chemical heterogeneity to neural specialization. Distal terminals of sensory neurons concerned with hearing, balance, taste, touch, and sight expressed glucuronyl 3-SO4, which apparently was present in an undefined glycoprotein. Some neurons in sensory nuclei of the brainstem exhibited a similar constituent on their surfaces. Retinal rod outer segments and the cerebellar granular layer possessed masked glucuronyl 3-SO4 that became immunopositive after digestion with chondroitinase ABC and that occurred in chondroitin 6-SO4 and chondroitin 4-SO4, respectively. The surface of neurons in the eighth nerve root and in neighboring nodes of Ranvier stained for unmasked glucuronic acid 3-SO4 and chondroitin 6-SO4. Some neurons of the cerebral cortex expressed unmasked glucuronyl 3-SO4, chondroitin 6-SO4, and terminal alpha GalNAc on their surfaces. Certain cortical neurons and nerve tracts with chondroitin 6-SO4 and terminal alpha GalNAc lacked glucuronyl 3-SO4, and other neurons possessing chondroitin 6-SO4 failed to express either glucuronyl 3-SO4 or terminal alpha GalNAc. Lability of lectin affinity to hyaluronidase suggested the presence of terminal alpha GalNAc in the chondroitin 6-SO4 on cortical neurons. The findings document further the heterogeneity of neural glycoconjugates, expand knowledge about the diversity of neurons with respect to their content of partially characterized glycoconjugates, and link glucuronyl 3-SO4 with or without chondroitin 6-SO4 spatially to sites of active Na+ transport in sensory nerves.

show abstract

Section: Variability Among Neurons In Glycoconjugate Contentmentioning

confidence: 68%

Section: Imentioning

confidence: 93%

Differentiation of glycoconjugates localized to sensory terminals and selected sites in brain

Spicer¹,

Naegele²,

Schulte³

1996

J. Comp. Neurol.

View full text Add to dashboard Cite

show abstract

“…Chondroitin sulfates have been shown to be localized in the perineuronal nets using monoclonal antibodies which recognize a stub structure created on the core protein of chondroitin sulfate proteoglycans after chondroitinase ABC treatment [5-7, 12, 23, 33, 43]. Some monoclonal antibodies which recognize specific epitopes on the chondroitin sulfate chains also stained cell surfaces of a subset of neurons [4,15,24,32,42,44]. Molecular species of the net-forming chondroitin sulfate proteoglycan have been demonstrated using antibodies which recognize core proteins of these proteoglycans.…”

Section: Proteoglycans In Perineuronal Netsmentioning

confidence: 99%

Proteoglycans in Perineuronal Nets.

Matsui¹,

Nishizuka

Oohira³

1999

Acta Histochem. Cytochem

View full text Add to dashboard Cite

“…Other MAbs have been raised against proteoglycans [7,31] and specifically against chondroitin 4-SO4 and chondroitin 6-SO4 [4]. These antibodies also were found to stain the surface of selected neurons in brain [3,7,11,31].…”

Section: Differentiation Of Glycoconjugates In Thementioning

confidence: 99%

Abundance and Diversity of Glycoconjugates Visualized Cytochemically in the Nervous System.

Spicer

Schulte

1995

Acta Histochem. Cytochem

View full text Add to dashboard Cite

A monoclonal antibody identifies a novel epitope surrounding a subpopulation of the mammalian central neurons

Cited by 57 publications

References 20 publications

Differentiation of glycoconjugates localized to sensory terminals and selected sites in brain

Differentiation of glycoconjugates localized to sensory terminals and selected sites in brain

Proteoglycans in Perineuronal Nets.

Abundance and Diversity of Glycoconjugates Visualized Cytochemically in the Nervous System.

Contact Info

Product

Resources

About