A monoclonal antibody (Po66) directed against human lung squamous cell carcinoma immunolocalization of tumour xenografts in nude mice

Abstract

Po66, a mouse IgG1 monoclonal antibody, was produced by immunization against a patient lung squamous cell carcinoma. The tissue reactivity of the antibody was measured by a radioimmunological assay with enzymatically dissociated cells, by an immunofluorescence test on frozen tissue sections and by peroxidase-staining of paraffin sections. The antibody bound to lung squamous cell carcinoma, oesophagus carcinoma and, inconsistently to lung adenocarcinoma but not to the other tumours tested. Some normal tissues also reacted positively, in particular bronchial serous glands, oesophagus epithelium and renal distal and collecting tubules. In normal and malignant tissues showing epithelioid differentiation, Po66 bound to the intermediate maturation area. The antigen immunoprecipitated by Po66 from lung squamous cell carcinoma appeared as a single band with a molecular weight 47,000 to 50,000 daltons. Purified monoclonal antibody Po66 and an unrelated IgG1 immunoglobulin were labelled with radioactive iodine and injected i.v. into nude mice bearing subcutaneous xenografts of human lung squamous cell carcinoma. The localization index in the tumour was 3.3. Antibody labelled with 131I allowed gamma-scintigraphic imaging of the xenografts which were clearly outlined by days 9 to 11.

Immunoscintigraphy of human lung squamous cell carcinoma using an iodine-131 labelled monoclonal antibody (Po66)

“…The non-specific retention of circulating proteins or macromolecules in cancer has been documented and seems related to the particularities of vasculature of tumours (Jain, 1987;O'Connor & Bale 1984). In our own study, the SLI measurements performed showed (Dazord et al, 1987), although most studies show a decrease of SLI in humans when compared to animal studies.…”

“…Monoclonal antibody and radiolabelling procedure Po66, an IgG I McAb, immunoprecipitated a 47 kDa molecular weight antigen and was prepared as reported earlier (Dazord et al, 1987 (Dazord et al, 1987).…”

“…Anti-CEA McAb (Riva et al, 1986), anti-osteosarcoma (Perkins et al, 1986), an antibody directed against c-myc oncogene product (Chan et al, 1986) and Fab'2 fragments directed against hepatitis virus (Kalofonos et al, 1988) have been used successfully to this end. In the present work, the first purpose was to determine whether Po66, a McAb prepared by immunisation against lung squamous cell carcinoma (LSqCC) (Dazord et al, 1987), was more effective than an unrelated immunoglobulin for NSCLC imaging. The second objective was to evaluate the value of immunoscintigraphy (IS) among other investigative means in the diagnosis of NSCLC.…”

Radioimmunoimaging of Non-Small Cell Lung Cancer with111In- and99MTc-Labeled Monoclonal Anti-Cea-Antibodies

“…Monoclonal antibodies against other pulmonary cancer cell antigens have been produced, and tested in xenograft studies (27)(28)(29). The monoclonal antibody 600Dll labeled with 13'1 was found to be specific for human small cell lung cancer (NCI-H69) and an adenoma cell line (A549) when tested in mouse xenografts (30).…”

Monoclonal antibody Po66 uptake by human lung tumours implanted in nude mice: effect of co-administration with doxorubicin

“…Production and radioiodination of monoclonal antibodies MAb Po66, a mouse IgGI, was obtained as described previously (Dazord et al, 1987 Figure la. show that the peak accumulation occurred between days 3 and 5. with 6.4 ± 1.3 and 7.1 ± 0.8% ID g-' of tumour respectively (mean ± s.d.).…”