A monoclonal antibody reactive with a common epitope of Moraxella (Branhamella) catarrhalis lipopolysaccharides

Oishi, Kazunori; Tanaka, Hirofumi; Sonoda, Fuminari; Borann, S; Ahmed, Kamruddin; Utsunomiya, Yoshiaki; Watanabe, Kiwao; Nagatake, Tsuyoshi; Vaneechoutte, Mario; Verschraegen, Gerda; Matsumoto, Keizō

doi:10.1128/cdli.3.3.351-354.1996

Cited by 11 publications

(1 citation statement)

References 31 publications

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“…A mAb reactive with a common epitope of M. catarrhalis LPS has been described [41]. It was suggested that the epitope differs from the serotype determinants.…”

Section: Presence Of Similar Saccharide Structures In Other Bacteria mentioning

confidence: 99%

The lipopolysaccharide of Moraxella catarrhalis

Holme

Rahman

Jansson

et al. 1999

European Journal of Biochemistry

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Moraxella catarrhalis has recently been shown to be both widespread and pathogenic, in contrast to previous reports. Several factors have been suggested as virulence factors, lipopolysaccharide (LPS) being one. Recent studies have shown the LPS to be without the O-chain, i.e. the polysaccharide part, and to have specific structural features corresponding to each of the three serogroups, A, B and C. The structures resemble in many respects those present in other Gram-negative nonenteric bacteria, with a galabiosyl element as a prominent common denominator. The presence of such common structures suggests that the LPS of these bacteria might be a part of a mechanism of survival for bacteria colonizing the human host.Keywords: Moraxella catarrhalis; antigen; structure; NMR; monoclonal antibodies; cross reactions Moraxella catarrhalis, once considered as a nonpathogenic respiratory tract commensal, is increasingly recognized as an important human pathogen [1,2]. It is now reported as the third most common isolate after Streptococcus pneumoniae and Haemophilus influenzae as the causative agent of otitis media in children [1,3,4] as well as lower respiratory tract infections such as bronchitis and pneumonia in the elderly, especially in those with underlying pulmonary disease [1,2,5]. In addition to these common infections, sporadic cases of ophthalmia neonatorum, conjunctivitis, keratitis, urethritis, peritonitis, meningitis, endocarditis and septicemia caused by M. catarrhalis have been reported [2]. Etiological diagnosis of infections with M. catarrhalis in children is invalidated by the high carrier rate in this age group [4,6±8]. A carrier rate of up to 56% has been reported in healthy children, whereas low carrier rates have been reported in adults [8]. As a consequence of repeated infections in childhood, antibody titers against M. catarrhalis are found from an early age in the whole population [9].In search for virulence factors of this bacterium, attention has been paid to surface exposed antigens such as lipopolysaccharide (LPS) [10], outer membrane proteins [11±13], and pili [14±16]. Recently, haemagglutinating ability has been found to correlate to disease caused by M. catarrhalis [17,18]. The factors influencing the pathogenesis of M. catarrhalis infection are not well understood. Only humans seem to be susceptible to infection by M. catarrhalis, and analysis of the immune response has been hampered by the lack of an adequate animal model. Purified LPS from M. catarrhalis has lethal activity for mice, comparable to that of enterobacterial LPS [10]. The free lipid A of two strains of M. catarrhalis shared some common antigenic properties with enterobacterial lipid A [10]. The LPS of M. catarrhalis consists of an oligosaccharide part and lipid A but lacks O-antigenic side chains consisting of repeating units [19,20] which are characteristic of Gram-negative enteric pathogens. In this regard, the LPS of M. catarrhalis resembles that of some other nonenteric Gram-negative bacteria, such as H. influenzae and Ne...

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“…A mAb reactive with a common epitope of M. catarrhalis LPS has been described [41]. It was suggested that the epitope differs from the serotype determinants.…”

Section: Presence Of Similar Saccharide Structures In Other Bacteria mentioning

confidence: 99%

The lipopolysaccharide of Moraxella catarrhalis

Holme

Rahman

Jansson

et al. 1999

European Journal of Biochemistry

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The Genus Moraxella

Hays¹

2006

The Prokaryotes

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Bactericidal Monoclonal Antibody Against Moraxella catarrhalis Lipooligosaccharide Cross-Reacts with Haemophilus Spp.

et al. 2007

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Monoclonal antibodies (MAbs) against lipooligosaccharide (LOS) determinants after immunization of BALB/c mice with heat inactivated Moraxella catarrhalis serotype A were generated. MAb 219A9 was specific for a common epitope of A, B, and C M. catarrhalis serotypes in ELISA and immunofluorescent test (IFT). In both tests it also cross-reacted with whole bacteria and LPS antigens isolated from non-typeable H. influenzae and H. parainfluenzae strains. IgM antibody clone 219A9 possessed a strong bactericidal effect against the three serotypes in the presence of complement. Our results demonstrate that antibodies directed to a single LOS epitope common for A, B, and C serotype could be highly protective. This suggests that the common determinants are very promising in the development of LOS-based vaccine against M. catarrhalis. The cross-reactions of MAb 219A9 with Haemophilus spp. also show that immunization could result in immune response to epitopes conserved in other important respiratory pathogens.

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A monoclonal antibody reactive with a common epitope of Moraxella (Branhamella) catarrhalis lipopolysaccharides

Cited by 11 publications

References 31 publications

The lipopolysaccharide of Moraxella catarrhalis

The lipopolysaccharide of Moraxella catarrhalis

The Genus Moraxella

Bactericidal Monoclonal Antibody Against Moraxella catarrhalis Lipooligosaccharide Cross-Reacts with Haemophilus Spp.

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