A morphometric model to minimize subjectivity in the histological assessment of hepatocellular carcinoma and its precursors in cirrhosis

Vertemati, Maurizio; Vizzotto, Laura; Moscheni, Claudia; Dhillon, A.P.; Dhillon, Amar P.; Quaglia, Alberto

doi:10.1002/jemt.20595

Cited by 11 publications

(7 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since I, II, and III correspond to the number of stages of change, the mean HAI was divided into five categories: 0–10 = normal tissue; 11–20 = mild to moderate damage to the tissue; 21–50 = moderate to severe damage to the tissue; 51–100 = severe damage to the tissue; and greater than 100 = irreparable damage to the tissue. A morphometric model [ 35 , 36 ] was also applied to obtain the volume, area, and diameter parameters of hepatocytes. Images of liver histopathological sections of three animals in each group were made with ×40 objective lens, resulting in nine images per group.…”

Section: Methodsmentioning

confidence: 99%

Antitumor effect of free rhodium (II) citrate and rhodium (II) citrate-loaded maghemite nanoparticles on mice bearing breast cancer: a systemic toxicity assay

et al. 2014

View full text Add to dashboard Cite

Breast cancer is one of the most prevalent cancer types among women. The use of magnetic fluids for specific delivery of drugs represents an attractive platform for chemotherapy. In our previous studies, it was demonstrated that maghemite nanoparticles coated with rhodium (II) citrate (Magh-Rh2Cit) induced in vitro cytotoxicity and in vivo antitumor activity, followed by intratumoral administration in breast carcinoma cells. In this study, our aim was to follow intravenous treatment to evaluate the systemic antitumor activity and toxicity induced by these formulations in Balb/c mice bearing orthotopic 4T1 breast carcinoma. Female Balb/c mice were evaluated with regard to toxicity of intravenous treatments through analyses of hemogram, serum levels of alanine aminotransferase, iron, and creatinine and liver, kidney, and lung histology. The antitumor activity of rhodium (II) citrate (Rh2Cit), Magh-Rh2Cit, and maghemite nanoparticles coated with citrate (Magh-Cit), used as control, was evaluated by tumor volume reduction, histology, and morphometric analysis. Magh-Rh2Cit and Magh-Cit promoted a significant decrease in tumor area, and no experimental groups presented hematotoxic effects or increased levels of serum ALT and creatinine. This observation was corroborated by the histopathological examination of the liver and kidney of mice. Furthermore, the presence of nanoparticles was verified in lung tissue with no morphological changes, supporting the idea that our nanoformulations did not induce toxicity effects. No studies about the systemic action of rhodium (II) citrate-loaded maghemite nanoparticles have been carried out, making this report a suitable starting point for exploring the therapeutic potential of these compounds in treating breast cancer.

show abstract

Section: Methodsmentioning

confidence: 99%

Antitumor effect of free rhodium (II) citrate and rhodium (II) citrate-loaded maghemite nanoparticles on mice bearing breast cancer: a systemic toxicity assay

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Regarding the former, a precise quantification of fibrotic tissue, both in clinical studies and experimental models, has been pursued over the last decade (Dahab et al 2004). Recently, it was shown that the relative volume, determined by pointcounting, is undoubtedly useful in the evaluation of hepatic fibrosis (Vertemati et al 2004), diagnosis of hepatocellular carcinoma (Vertemati et al 2008), and in assessing hepatic steatosis in liver biopsies (Catta-Preta et al 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, it was shown that the relative volume, determined by point‐counting, is undoubtedly useful in the evaluation of hepatic fibrosis (Vertemati et al. 2004), diagnosis of hepatocellular carcinoma (Vertemati et al. 2008), and in assessing hepatic steatosis in liver biopsies (Catta‐Preta et al.…”

Section: Introductionmentioning

confidence: 99%

The use of design‐based stereology to evaluate volumes and numbers in the liver: a review with practical guidelines

2012

View full text Add to dashboard Cite

Stereology offers a number of tools for the analysis of sections in microscopy (which usually provide only twodimensional information) for the purpose of estimating geometric quantities, such as volume, surface area, length or number of particles (cells or other structures). The use of these tools enables recovery of the threedimensional information that is inherent in biological tissues. This review uses the liver as a paradigm for summarizing the most commonly used state-of-the-art methods for quantitation in design-based stereology. Because it is often relevant to distinguish hyperplasia and hypertrophy in liver responses, we also focus on potential pitfalls in the sampling and processing of liver specimens for stereological purposes, and assess the existing methods for volume and number estimation. With respect to volume, we considered whole liver volume (V), volume density (V V ) and so-called local volumes, including the number-weighted volume ( v N ) and the volume-weighted volume ( v v ). For number, we considered the total number (N) and the numerical density (N V ). If correctly applied, current stereological methods guarantee that no bias is introduced in the estimates, which will be therefore accurate; additionally, methods can be tuned for obtaining precise quantitative estimates that can reveal subtle changes in the volume or number of selected hepatic cells. These methods have already detailed the effects of some substances and specific diets on the liver, and should be routinely included in the toolbox of liver research.

show abstract

“…The grading of fibrosis in general and namely of reticulin fibers is of main interest in bone marrow pathologies [86] and fibrous diseases of the liver [87]. For this reason, different methods for the quantification of reticular fibrosis have been developed, some of them consisting in automated morphometry [88][89][90][91][92][93]. However, these methods quantify the percentage of stained area and we considered that not only the amount of fibers, but also the morphometric features were relevant, given that these are usually subjectively assessed.…”

Section: Option 2: To Use Commercial Software For Image Analysis Whicmentioning

confidence: 99%

Tumor Microenvironment Heterogeneity: A Review of the Biology Masterpiece, Evaluation Systems, and Therapeutic Implications

Tadeo¹,

Álvaro²,

Navarro³

et al. 2016

Composition and Function of the Extracellular Matrix in the Human Body

View full text Add to dashboard Cite

A tumor can be considered as a highly heterogeneous functional tissue, connected and dependent on the microenvironment, which sends and receives signals to and from the tumor tissue itself. Tumor cells alter the mechanical properties of the microenvironment in order to create favorable conditions for their proliferation. Stromal cells and noncellular elements of the extracellular matrix (ECM), including the host immune system, the fibrous scaffolding, the fundamental substance, and blood vascularization can determine tumoral cell morphologies, functions, aggressiveness, and response to treatment, as well as an accurate assessment of prognosis of the patients. Robust morphometric digital pathology techniques that are able to standardize measurements and analyse whole sets of immunohistochemical images are called for to identify, describe, and quantify the elements of the ECM. The computer-automated segmentation algorithms are therefore required to increase the knowledge on the tumor microenvironment heterogeneity and to provide new therapeutic targets.

show abstract

A morphometric model to minimize subjectivity in the histological assessment of hepatocellular carcinoma and its precursors in cirrhosis

Abstract: Our novel hybrid classification tool may minimize subjectivity in the histological assessment of nodular lesions in cirrhosis.

Cited by 11 publications

References 15 publications

Antitumor effect of free rhodium (II) citrate and rhodium (II) citrate-loaded maghemite nanoparticles on mice bearing breast cancer: a systemic toxicity assay

Antitumor effect of free rhodium (II) citrate and rhodium (II) citrate-loaded maghemite nanoparticles on mice bearing breast cancer: a systemic toxicity assay

The use of design‐based stereology to evaluate volumes and numbers in the liver: a review with practical guidelines

Tumor Microenvironment Heterogeneity: A Review of the Biology Masterpiece, Evaluation Systems, and Therapeutic Implications

Contact Info

Product

Resources

About