A moso bamboo transcription factor, Phehdz1, positively regulates the drought stress response of transgenic rice

Gao, Yameng; Liu, Huanlong; Zhang, Kaimei; Li, Fēi; Wu, Min; Xiang, Yan

doi:10.1007/s00299-020-02625-w

Cited by 26 publications

(18 citation statements)

References 94 publications

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“…The bacterial fluid was then injected into tobacco leaves with a needle. The treated tobacco was instantaneously expressed in the dark for 36-40 h [ 66 , 67 ]. Finally, each sample was observed with the help of a confocal laser scanning microscope (CarlZeiss LSM710, Jena, Germany).…”

Section: Methodsmentioning

confidence: 99%

Homologous Drought-Induced 19 Proteins, PtDi19-2 and PtDi19-7, Enhance Drought Tolerance in Transgenic Plants

Lin

Chen

et al. 2022

IJMS

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Drought-induced 19 (Di19) proteins play important roles in abiotic stress responses. Thus far, there are no reports about Di19 family in woody plants. Here, eight Di19 genes were identified in poplar. We analyzed phylogenetic tree, conserved protein domain, and gene structure of Di19 gene members in seven species. The results showed the Di19 gene family was very conservative in both dicotyledonous and monocotyledonous forms. On the basis of transcriptome data, the expression patterns of Di19s in poplar under abiotic stress and ABA treatment were further studied. Subsequently, homologous genes PtDi19-2 and PtDi19-7 with strong response to drought stress were identified. PtDi19-2 functions as a nuclear transcriptional activator with a transactivation domain at the C-terminus. PtDi19-7 is a nuclear and membrane localization protein. Additionally, PtDi19-2 and PtDi19-7 were able to interact with each other in yeast two-hybrid system. Overexpression of PtDi19-2 and PtDi19-7 in Arabidopsis was found. Phenotype identification and physiological parameter analysis showed that transgenic Arabidopsis increased ABA sensitivity and drought tolerance. PtDi19-7 was overexpressed in hybrid poplar 84K (Populus alba × Populus glandulosa). Under drought treatment, the phenotype and physiological parameters of transgenic poplar were consistent with those of transgenic Arabidopsis. In addition, exogenous ABA treatment induced lateral bud dormancy of transgenic poplar and stomatal closure of transgenic Arabidopsis. The expression of ABA/drought-related marker genes was upregulated under drought treatment. These results indicated that PtDi19-2 and PtDi19-7 might play a similar role in improving the drought tolerance of transgenic plants through ABA-dependent signaling pathways.

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Section: Methodsmentioning

confidence: 99%

Homologous Drought-Induced 19 Proteins, PtDi19-2 and PtDi19-7, Enhance Drought Tolerance in Transgenic Plants

Lin

Chen

et al. 2022

IJMS

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“…Access to the moso bamboo genome [210,211] provides a chance to many researchers for genome-wide classifications of TFs such as aquaporin, AAAP, UBP, IQD, HD-Zip, Hexokinase, Aux/IAA and ARF, NAC, PeUGE, HSF, and CONSTANS-like in moso bamboo [188,[214][215][216][217][218][219][220][221][222][223]. Genome-wide classifications of TFs' families in moso bamboo have been carried out and have demonstrated a limited molecular characterization in model plants by exogenous gene transfer in rice and Arabidopsis and the expression profile against stresses [224][225][226]. The expression of PeLAC was high in stem and its promoter sequence contains ABRE cisregulatory element that responds to ABA and GA treatments by upregulating and downregulating the transcript of PeLAC, respectively [227].…”

Section: Understanding the Bamboos' Tolerance Using Ngs And Metabolomementioning

confidence: 99%

“…Genome-wide classifications of TFs’ families in moso bamboo have been carried out and have demonstrated a limited molecular characterization in model plants by exogenous gene transfer in rice and Arabidopsis and the expression profile against stresses [ 224 , 225 , 226 ]. The expression of PeLAC was high in stem and its promoter sequence contains ABRE cisregulatory element that responds to ABA and GA treatments by upregulating and downregulating the transcript of PeLAC , respectively [ 227 ].…”

Section: Understanding the Bamboos’ Tolerance Using Ngs And Metabolomementioning

confidence: 99%

“…The accumulation of metabolites also helps plants to encounter stress conditions. An increase in anthocyanin contents was examined in Ma bamboo in the overexpressing leaf-color-related gene, which improved stress resilience against cold and drought conditions by enhancing stress-related antioxidant activity [ 225 ]. Very limited research is available on the heterologous system investigations of genes retrieved using transcriptome data of bamboo [ 228 , 234 , 235 ].…”

Section: Understanding the Bamboos’ Tolerance Using Ngs And Metabolomementioning

confidence: 99%

“…A significant expression alteration of Phehdz1 was induced by drought, salinity, and ABA treatments, but the high expression was in roots. Overexpressing transgene rice (OE-Phehdz1) displayed improved DS tolerance and altered secondary metabolism [ 225 ]. The lignification of tissues in bamboo is a unique character that contains monolignol glucosides (i.e., syringin, coniferin, p-glucocoumaryl alcohol, and guaiacyl) that have been examined as the key storage components of lignin precursors, which are transported to the outer cells [ 236 , 237 ].…”

Section: Understanding the Bamboos’ Tolerance Using Ngs And Metabolomementioning

confidence: 99%

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Entailing the Next-Generation Sequencing and Metabolome for Sustainable Agriculture by Improving Plant Tolerance

Hou

Hussain

Imran

et al. 2022

IJMS

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Crop production is a serious challenge to provide food for the 10 billion individuals forecasted to live across the globe in 2050. The scientists’ emphasize establishing an equilibrium among diversity and quality of crops by enhancing yield to fulfill the increasing demand for food supply sustainably. The exploitation of genetic resources using genomics and metabolomics strategies can help generate resilient plants against stressors in the future. The innovation of the next-generation sequencing (NGS) strategies laid the foundation to unveil various plants’ genetic potential and help us to understand the domestication process to unmask the genetic potential among wild-type plants to utilize for crop improvement. Nowadays, NGS is generating massive genomic resources using wild-type and domesticated plants grown under normal and harsh environments to explore the stress regulatory factors and determine the key metabolites. Improved food nutritional value is also the key to eradicating malnutrition problems around the globe, which could be attained by employing the knowledge gained through NGS and metabolomics to achieve suitability in crop yield. Advanced technologies can further enhance our understanding in defining the strategy to obtain a specific phenotype of a crop. Integration among bioinformatic tools and molecular techniques, such as marker-assisted, QTLs mapping, creation of reference genome, de novo genome assembly, pan- and/or super-pan-genomes, etc., will boost breeding programs. The current article provides sequential progress in NGS technologies, a broad application of NGS, enhancement of genetic manipulation resources, and understanding the crop response to stress by producing plant metabolites. The NGS and metabolomics utilization in generating stress-tolerant plants/crops without deteriorating a natural ecosystem is considered a sustainable way to improve agriculture production. This highlighted knowledge also provides useful research that explores the suitable resources for agriculture sustainability.

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The TCP transcription factor PeTCP10 modulates salt tolerance in transgenic Arabidopsis

Liu

Gao

et al. 2021

Plant Cell Rep

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A moso bamboo transcription factor, Phehdz1, positively regulates the drought stress response of transgenic rice

Cited by 26 publications

References 94 publications

Homologous Drought-Induced 19 Proteins, PtDi19-2 and PtDi19-7, Enhance Drought Tolerance in Transgenic Plants

Homologous Drought-Induced 19 Proteins, PtDi19-2 and PtDi19-7, Enhance Drought Tolerance in Transgenic Plants

Entailing the Next-Generation Sequencing and Metabolome for Sustainable Agriculture by Improving Plant Tolerance

The TCP transcription factor PeTCP10 modulates salt tolerance in transgenic Arabidopsis

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