A mouse monoclonal antibody against a polymorphic determinant in a defined subset of DR molecules

Mazzilli, Maria Cristina; Tanigaki, Nobuyuki; Cascino, Isabella; Porrini, Sandro Costanzi; Trabace, S; Cappellacci, Sandra; Testa, Luana; Gandini, E.

doi:10.1016/0198-8859(86)90043-1

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…2). These findings suggest that the high frequency of crossreactive alloreactive T cell clones is unlikely to be explained by widely spread antigen fragments bound in the groove of the HLA-DR 1 and HLA-DR4 molecules (Table III) (35). Support for this mechanism comes from a recent report describing a sequence homology between a hexapeptide of the EBV-encoded gpl 10 and the third hvr ofdisease-associated haplotypes (36).…”

Section: Resultsmentioning

confidence: 65%

Disease-associated human histocompatibility leukocyte antigen determinants in patients with seropositive rheumatoid arthritis. Functional role in antigen-specific and allogeneic T cell recognition.

Weyand

Goronzy²

1990

J. Clin. Invest.

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The susceptibility to develop seropositive rheumatoid arthritis (RA) has been linked to specific genomic polymorphisms within the HLA complex. Two different haplotypes have been associated with the disease, HLA-DR1 and HLA-DR4. To investigate the link between such phenotypic disease associations and potential immune mechanisms we used alloreactive and antigen-specific human T cell clones. Here we describe a panel of alloreactive T cell clones directed to polymorphic determinants encoded by the third hypervariable region (hvr) of the HLA-DR #,-chain. T cell determinants defined by these clones are shared among HLA-DR1, HLA-Dw4, HLA-Dw13, HLA-Dw14, and HLA-Dw15, and are frequent in a population of RA patients. To study the role of such disease-associated epitopes in antigen-restricted T cell recognition we generated T cell clones from RA patients specific for mycobacterial antigens, Epstein-Barr virus antigens, and tetanus toxoid. In all three antigenic systems T cell clones were restricted to either HLA-DR1 or HLA-DR4. These data suggest that the polymorphisms within the first and second hvr of the HLA-DR B,-chain that are distinct in HLA-DR1 and HLA-DR4 and not associated with the disease are crucially involved in the recognition of antigens. Polymorphic determinants encoded by the third hvr are shared among disease-associated haplotypes and may function to mediate the interaction of alloreactive T cell receptor molecules with the HLA complex. (J. Clin. Invest.

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Section: Resultsmentioning

confidence: 65%

Disease-associated human histocompatibility leukocyte antigen determinants in patients with seropositive rheumatoid arthritis. Functional role in antigen-specific and allogeneic T cell recognition.

Weyand

Goronzy²

1990

J. Clin. Invest.

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“…It exhibits, in immunoprecipitation, a specificity wider than that ascribed by serology (Crumpton et al, 1985). The anti-DP mAb I-LRl recognizes a polymorphic determinant expressed by DP2positive cells and by a proportion of DP blanks (Nadler et al, 1980;Shaw et al, 1982). Transfection with HLA-DP cosmid clones and collaborative histocompatibility testing have demonstrated the restricted specificity of mAb B7/21 for DP products (Watson et al, 1983;Austin et al, 1985).…”

Section: Monoclonal Antibodies and Xenoantiseramentioning

confidence: 99%

mAb KUL/05 IDENTIFIES A DENATURATION‐RESISTANT DETERMINANT SHARED BY CLASS II MHC PRODUCTS DR, DQ AND DP

Giacomini

Tecce

Nicotra

et al. 1989

Int J Immunogenetics

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SUMMARY mAb KUL/05, a novel murine monoclonal antibody, reacts with molecules displaying the typical tissue distribution and molecular profile of class II MHC antigens. An extensive scrutiny employing serological and immunochemical assays on DR homozygous and DRα‐ mutant cell lines has shown that this reagent displays some additional, interesting features, namely mAb KUL/05 (a) binds in a broadly monomorphic fashion to cells of DR1 through seven specifities, (b) recognizes a determinant shared by a large proportion of DR, DQ and DPβ chains from most haplotypes, in both their monomeric and α chain‐associated forms, and (c) reacts with frozen, acetone‐fixed, as well as conventional, formalin‐fixed, paraffin embedded tissues. Thus, mAb KUL/05 is likely to represent a useful adjunct for the study of the expression of class II MHC products in normal and pathological tissue specimens.

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HLA‐DR association in rheumatoid arthritis and the shared susceptibility epitope hypothesis

Galeazzi

Cappellacci

Lulli

et al. 1989

Arthritis & Rheumatism

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A mouse monoclonal antibody against a polymorphic determinant in a defined subset of DR molecules

Cited by 3 publications

References 23 publications

Disease-associated human histocompatibility leukocyte antigen determinants in patients with seropositive rheumatoid arthritis. Functional role in antigen-specific and allogeneic T cell recognition.

Disease-associated human histocompatibility leukocyte antigen determinants in patients with seropositive rheumatoid arthritis. Functional role in antigen-specific and allogeneic T cell recognition.

mAb KUL/05 IDENTIFIES A DENATURATION‐RESISTANT DETERMINANT SHARED BY CLASS II MHC PRODUCTS DR, DQ AND DP

HLA‐DR association in rheumatoid arthritis and the shared susceptibility epitope hypothesis

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