A mouse monoclonal antibody detecting a DR-related MT2-like specificity: Serology and biochemistry

Koning, Frits; Schreuder, Ieke; Giphart, Marius J.; Bruning, Hans

doi:10.1016/0198-8859(84)90027-2

Cited by 88 publications

(31 citation statements)

References 16 publications

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“…This antibody has been shown elsewhere not to crossreact with MHC class I molecules [14]. Mouse IgG1 monoclonal antibody of irrelevant specificity was used as control.…”

Section: Anti-mhc Class II Monoclonal Antibodymentioning

confidence: 99%

ALeishmaniaHomologue of Receptors for Activated C‐Kinase (LACK) Induces Both Interferon‐γ and Interleukin‐10 in Natural Killer Cells of Healthy Blood Donors

et al. 2000

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Natural killer (NK) cells from individuals unexposed to Leishmania organisms proliferate with high interferon (IFN)-gamma secretion in response to crude Leishmania antigen preparations. In an attempt to identify the molecules that induce blood cells to proliferate and to secrete cytokines, we tested the effect of a 36-kDa Leishmania homologue of receptors for activated C-kinase (LACK) on peripheral blood mononuclear cells from unexposed individuals. Mainly CD8(+) and NK cells proliferated in response to LACK. At both the mRNA and soluble protein level, the main sources for LACK-induced IFN-gamma and interleukin (IL)-10 were T and NK cells. Furthermore, in the presence of anti-major histocompatibility complex (MHC) class II antibody, there was inhibition of LACK responses in both CD4(+) and CD16/56(+) cells, with a marked decrease in IFN-gamma but with an increase in IL-10 production. We conclude that the response to LACK is part of the response to Leishmania organisms in unexposed donors described elsewhere. That this NK-dominated response is MHC class II sensitive, whether through a direct or indirect effect, is discussed.

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“…This antibody has been shown elsewhere not to crossreact with MHC class I molecules [14]. Mouse IgG1 monoclonal antibody of irrelevant specificity was used as control.…”

Section: Anti-mhc Class II Monoclonal Antibodymentioning

confidence: 99%

ALeishmaniaHomologue of Receptors for Activated C‐Kinase (LACK) Induces Both Interferon‐γ and Interleukin‐10 in Natural Killer Cells of Healthy Blood Donors

et al. 2000

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“…Cells (10 s) were incubated in V-bottomed microtiter plates (Flow Laboratories) with 10 #1 of purified mAb (1 mg/ml) for 30 rain at 4~ After two washes with PBS containing 0.02 mM sodium azide and 1% BSA (Sigma Chemical Co., St. Louis, MO), the cells were incubated with 1:40 dilution of FITC-labeled F(ab')2 fragments of goat anti-mouse antibody (Tago, Inc., Burlingame, CA) for 30 min at 4~ After three additional washes, the labeled cell samples were analyzed by flow microfluorimetry (FMF) on a FACScan | (Becton Dickinson & Co., Sunnyvale, CA). The anti-MHC class II mAbs PdV5.2 (HLA-DR/ DP/DQ) (20), Q5/13 HLA-DK/DP (21), SPV-L3 (HLA-DQ) (14), and anti-CD3 (SPV-T3b) (14) were described previously).…”

Section: Methodsmentioning

confidence: 99%

Interleukin 10 (IL-10) and viral IL-10 strongly reduce antigen-specific human T cell proliferation by diminishing the antigen-presenting capacity of monocytes via downregulation of class II major histocompatibility complex expression.

Malefyt¹,

Haanen²,

Spits³

et al. 1991

The Journal of Experimental Medicine

1,789

874

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SummaryInterleukin 10 (IL-10) and viral Ibl0 (v-IL-10) strongly reduced antigen-specific proliferation of human T cells and CD4 + T cell clones when monocytes were used as antigen-presenting cells. In contrast, IL-10 and v-Ibl0 did not affect the proliferative responses to antigens presented by autologous Epstein-Barr virus-lymphoblastoid cell line (EBV-LCL). Inhibition of antigen-specific T cell responses was associated with downregulation of constitutive, as well as interferon 3'-or Ib4-induced, class II MHC expression on monocytes by IL-10 and v-Ibl0, resulting in the reduction in antigen-presenting capacity of these ceUs. In contrast, IL-10 and v-Ibl0 had no effect on class II major histocompatibility complex (MHC) expression on EBV-LCL. The reduced antigenpresenting capacity of monocytes correlated with a decreased capacity to mobilize intracellular Ca 2 + in the responder T cell clones. The diminished antigen-presenting capacities of monocytes were not due to inhibitory effects of II.-10 and v-Ibl0 on antigen processing, since the proliferative T cell responses to antigenic peptides, which did not require processing, were equaUy well inhibited. Furthermore, the inhibitory effects of Ibl0 and v-IL-10 on antigen-specific proliferative T cell responses could not be neutralized by exogenous Ib2 or Ib4. Although IL-10 and v-IL-10 suppressed IL-lc~, IL-1B, tumor necrosis factor ot (TNF-c~), and IL-6 production by monocytes, it was excluded that these cytokines played a role in antigen-specific T cell proliferation, since normal antigenspecific responses were observed in the presence of neutralizing anti-Ibl, -IL-6, and -TNF-tx mAbs. Furthermore, addition of saturating concentrations of IL-lot, IL-I~, IL-6, and TNF-o~ to the cultures had no effect on the reduced proliferative T cell responses in the presence of Ibl0, or v-Ibl0. Collectively, our data indicate that IL-10 and v-IL-10 can completely prevent antigen-specific T cell proliferation by inhibition of the antigen-presenting capacity of monocytes through downregulation of class II MHC antigens on monocytes.

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“…The DRw52 haplotypes usually carry three different Diogenes, of which two are expressed [10]. Both the DR/9I and DRyfflll gene products carry DRw52-associated epitopes, as defined by monoclonal antibodies [7,8] or T-cell clones [9]. The polymorphism of the DR^SI gene seems to correlate with the DR private specificities, whereas the DR/fflll gene has only limited polymorphism.…”

Section: Discussionmentioning

confidence: 99%

Matching for Supertypic DR Epitopes Does Not Reduce Mixed Lymphocyte Culture Reactivity

et al. 1987

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It was recently demonstrated that matching for the supertypic DR specificities DRw52 and DRw53 significantly improved graft survival in a group of retrospectively typed kidney transplant patients. We investigated whether this phenomenon was also reflected in vitro in the mixed lymphocyte culture (MLC) test system. MLC matrices were set up, but no effect of the matching between responder and stimulator for DRw52 and DRw53 was observed, although matching for the DR 'private' specificities (DR1-DRw10) was significant. In responder-stimulator combinations, too, which were mismatched for 1 DR antigen only, it did not matter whether or not the mismatched antigen on the stimulator was DRw52 or DRw53 compatible to the responder. These findings imply that matching for supertypic DR epitopes does not necessarily lead to reduced MLC reactivity.

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A mouse monoclonal antibody detecting a DR-related MT2-like specificity: Serology and biochemistry

Cited by 88 publications

References 16 publications

ALeishmaniaHomologue of Receptors for Activated C‐Kinase (LACK) Induces Both Interferon‐γ and Interleukin‐10 in Natural Killer Cells of Healthy Blood Donors

ALeishmaniaHomologue of Receptors for Activated C‐Kinase (LACK) Induces Both Interferon‐γ and Interleukin‐10 in Natural Killer Cells of Healthy Blood Donors

Interleukin 10 (IL-10) and viral IL-10 strongly reduce antigen-specific human T cell proliferation by diminishing the antigen-presenting capacity of monocytes via downregulation of class II major histocompatibility complex expression.

Matching for Supertypic DR Epitopes Does Not Reduce Mixed Lymphocyte Culture Reactivity

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