A multi-axis robot-based bioprinting system supporting natural cell function preservation and cardiac tissue fabrication

Zhang, Zeyu; Wu, Chenming; Dai, Chengkai; Shi, Qingqing; Fang, Guoxin; Xie, Dong; Zhao, Xiangjie; Liu, Yong-Jin; Wang, Charlie C. L.; Wang, Xiu‐Jie

doi:10.1016/j.bioactmat.2022.02.009

Cited by 33 publications

(18 citation statements)

References 22 publications

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“…The 7-axis serial robot used in this study has redundant properties (possessing 7-DoF, more than 3-DoF for the RCM constraint and 3-DoF for surgical tasks), which is capable of performing MIS under the RCM constraint in an active approach. Although more research on robotic bioprinting has been reported recently, the conventional 6-axis manipulators used in previous studies do not have task redundancy and cannot perform the above operations 10,27,44 . In addition to ful lling the RCM constraint, redundancy can also be exploited to achieve other additional tasks, such as obstacle avoidance, human-like behavior, and manipulability optimization in a human-machine cooperation scenario 45,46 , which further advances this therapeutic technique for tissue repair on the interior or exterior of the human body.…”

Section: Discussionmentioning

confidence: 99%

Subaqueous Bioprinting: A Novel Strategy for Fetal Membrane Repair with 7‐Axis Robot‐Assisted Minimally Invasive Surgery

Zhao

et al. 2022

Adv Funct Materials

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Premature rupture of membranes (PROM), de ned as breakage of the amniotic sac prior to delivery, complicates 2-4% of pregnancies, leads to one-third of preterm births, and increases the risk of perinatal mortality and morbidity in survivors. In vivo bioprinting enables the fabrication of three-dimensional (3D) structures directly for tissue repair or reconstruction in living animals but is challenging to perform in the subaqueous narrow space where fetal membranes (FMs) are located. This study reports a novel strategy consisting of an ultrafast photoresponsive hydrogel (1.5 s) and a 7-axis bioprinting robot to perform subaqueous in vivo bioprinting in a minimally invasive approach. Specially, a hydrogel patch with designed gel rivets is printed subaqueously for the therapeutic needs of PROM, and the patch displays robust tissue adhesion, favorable biocompatibility, mechanical properties resembling native tissues, and an appropriate sealing timescale to prolong pregnancy. Experiments performed in an in vitro uterus model and a mid-gestational rabbit model show a favorable sealing effect for PROM, which validates the potential of our subaqueous bioprinting approach for application. This study not only expands upon in vivo bioprinting techniques but also provides valuable insights for the treatment of PROM and other diseases involving tissue injury in clinical settings.

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Section: Discussionmentioning

confidence: 99%

Subaqueous Bioprinting: A Novel Strategy for Fetal Membrane Repair with 7‐Axis Robot‐Assisted Minimally Invasive Surgery

Zhao

et al. 2022

Adv Funct Materials

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“…( Huang et al, 2022 ). 2) In addition to sticking to VEGF, PDA coating can cling to bone progenitor cells, which promotes bone progenitor cell adhesion and reproduction ( Wu et al, 2022 ; Zhang et al, 2022 ). These factors of this paper are extremely important in clinical application and it is of great significance for clinical healing of bone defects.…”

Section: Discussionmentioning

confidence: 99%

Covalent immobilization of VEGF on allogeneic bone through polydopamine coating to improve bone regeneration

Huang

Lü

Liu

et al. 2022

Front. Bioeng. Biotechnol.

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Objective: Promoting bone regeneration and repairing in bone defects is of great significance in clinical work. Using a simple and effective surface treatment method to enhance the osteogenic ability of existing bone scaffold is a promising method. In this article, we study the application of catecholic amino acid 3,4-dihydroxyphenylalanine (DOPA) surface coating chelated with vascular endothelial growth factor (VEGF) on allogeneic bone.Method: Allogeneic bone is immersed in DOPA solution and DOPA form polydopamine (PDA) with good adhesion. Electron microscopy is used to characterize the surface characteristics of allogeneic bone. MC3T3-E1 cells were tested for biocompatibility and osteogenic signal expression. Finally, a 12-week rabbit bone defect model was established to evaluate bone regeneration capability.Results: We found that the surface microenvironment of DOPA bonded allogeneic bone was similar to the natural allogeneic bone. VEGF loaded allografts exhibited satisfying biocompatibility and promoted the expression of osteogenic related signals in vitro. The VEGF loaded allografts healed the bone defect after 12 weeks of implantation that continuous and intact bone cortex was observed.Conclusion: The PDA coating is a simple surface modification method and has mild properties and high adhesion. Meanwhile, the PDA coating can act on the surface modification of different materials. This study provides an efficient surface modification method for enhancing bone regeneration by PDA coating, which has a high potential for translational clinical applications.

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“…To improve the printing efficiency of constructing multilayered slicing structures, some researchers have developed a multiaxis Cartesian bioprinter with two individually controlled nozzles [8] or used multiple six-degree-of-freedom (6-DOF) robots that can print in parallel [9]. Although these methods have the ability to realize simultaneous bioprinting with two nozzles, they lack the ability to perform the bioprinting simultaneously with more printing methods.…”

Section: Modular Designed Platform For Simultaneous Printingmentioning

confidence: 99%

Simultaneous multimaterial multimethod bioprinting

et al. 2022

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Bioprinting is an important technology in the field of biofabrication that aims to create functional biomimetic structures. The flexibility, versatility and functionality of bioprinting enable the fabrication of intricate biological structures by combining cells, biomaterials or growth factors as printing bioinks [1,2]. Conventional bioprinting technologies now face challenges, including the contradiction between the overall mechanical stability and the biological microenvironment of the construct and the vascularization of the printed tissues/organs. People have come to realize that it is unrealistic to build fully functional tissues/organs with just a single material. The complex composition and diversity of native tissues/organs require more than what a single-material bioprinting technique can possibly deliver [3].

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A multi-axis robot-based bioprinting system supporting natural cell function preservation and cardiac tissue fabrication

Cited by 33 publications

References 22 publications

Subaqueous Bioprinting: A Novel Strategy for Fetal Membrane Repair with 7‐Axis Robot‐Assisted Minimally Invasive Surgery

Subaqueous Bioprinting: A Novel Strategy for Fetal Membrane Repair with 7‐Axis Robot‐Assisted Minimally Invasive Surgery

Covalent immobilization of VEGF on allogeneic bone through polydopamine coating to improve bone regeneration

Simultaneous multimaterial multimethod bioprinting

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