A multi-enzyme-like activity exhibiting mussel-inspired nanozyme hydrogel for bacteria-infected wound healing

Zhu, Junrun; Han, Qinqin; Li, Qiulan; Wang, Fang; Dong, Miaodan; Liu, Nuoya; Li, Xiao; Chen, Dan; Yang, Dezhi; Song, Yuzhu; Yang, Yaling

doi:10.1039/d2bm02004a

Cited by 12 publications

(5 citation statements)

References 60 publications

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“…The fluorescent probe DCFH-DA can penetrate the cell membrane and bind to bacterial ROS. 68 As presented in Figure 4E, we found that the MoO 3−x /Cu group was treated with ROS generation; in addition, H 2 O 2 promoted ROS formation in the MoO 3−x /Cu group. The large amount of ROS generated by the polymerase activity of the nanoenzymes can be depleted by GSH, and the concentration at the site of infection is significantly increased due to the abnormal encapsulation of extracellular polymerized Merrick substance.…”

Section: Determination Of Reactive Oxygen Speciesmentioning

confidence: 61%

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Ultralow Loading Copper-Intercalated MoO₃ Nanobelts with High Activity against Antibiotic-Resistant Bacteria

Liu,

Zuo,

et al. 2024

ACS Appl. Mater. Interfaces

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In recent years, the infection rate of antibiotic resistance has been increasing year by year, and the prevalence of super bacteria has posed a great threat to human health. Therefore, there is an urgent need to find new antibiotic alternatives with long-term inhibitory activity against a broad spectrum of bacteria and microorganisms in order to avoid the proliferation of more multidrug-resistant (MDR) bacteria. The presence of natural van der Waals (vdW) gaps in layered materials allows them to be easily inserted by different guest species, providing an attractive strategy for optimizing their physicochemical properties and applications. Here, we have successfully constructed a copper-intercalated α-MoO 3 nanobelt based on nanoenzymes, which is antibacterial through the synergistic effect of multiple enzymes. Compared with α-MoO 3 , MoO 3−x /Cu nanobelts with a copper loading capacity of 2.11% possess enhanced peroxidase (POD) catalytic activity and glutathione (GSH) depletion, indicating that copper intercalation significantly improves the catalytic performance of the nanoenzymes. The MoO 3−x /Cu nanobelts are effective in inducing POD and oxidase (OXD) and catalase (CAT) activities in the presence of H 2 O 2 and O 2 , which resulted in the generation of large amounts of reactive oxygen species (ROS), which were effective in bacterial killing. Interestingly, MoO 3−x /Cu nanobelts can serve as glutathione oxidase (GSHOx)-like nanoenzymes, which can deplete GSH in bacteria and thus significantly improve the bactericidal effect. The multienzyme-catalyzed synergistic antimicrobial strategy shows excellent antimicrobial efficiency against β-lactamase-producing Escherichia coli (ESBL-E. coli) and methicillin-resistant Staphylococcus aureus (MRSA). MoO 3−x /Cu exhibits excellent spectral bactericidal properties at very low concentrations (20 μg mL −1 ). Our work highlights the wide range of antibacterial and antiinfective biological applications of copper-intercalated MoO 3−x /Cu nanobelt catalysts.

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Section: Determination Of Reactive Oxygen Speciesmentioning

confidence: 61%

“…ROS can damage DNA, proteins, and bacterial walls. The fluorescent probe DCFH-DA can penetrate the cell membrane and bind to bacterial ROS . As presented in Figure E, we found that the MoO 3– x /Cu group was treated with ROS generation; in addition, H 2 O 2 promoted ROS formation in the MoO 3– x /Cu group.…”

Section: Resultsmentioning

confidence: 99%

Ultralow Loading Copper-Intercalated MoO₃ Nanobelts with High Activity against Antibiotic-Resistant Bacteria

Liu,

Zuo,

et al. 2024

ACS Appl. Mater. Interfaces

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“…Thus, different nanomaterials were explored that exhibit CAT-like activities such as nCe, iron oxides, AuNPs, and cobalt oxide NPs [ 118 ]. For instance, a multifunctional hydrogel with a multienzyme-like activity was developed by combining mussel-inspired carbon dot reduced-Ag (CDs/AgNPs) and Cu/Fe-nitrogen-imbued carbon (Cu, Fe-NC) [ 119 ]. The NZ exhibited both GSH depletion and OXD-like activity that resulted in hydrogels with exceptional antibacterial properties.…”

Section: Enzymatic Mechanisms and Multifunctional Roles Of Nz@hydrogelsmentioning

confidence: 99%

“…In another study, Zhu et al developed a hydrogel with multiple enzyme-like activities using CDs/AgNPs and Cu, Fe-NC [ 119 ]. During the inflammatory stage of wound healing, the system functioned as a CAT mimic, generating sufficient O 2 by catalyzing intracellular H 2 O 2 to alleviate hypoxia.…”

Section: Enzymatic Mechanisms and Multifunctional Roles Of Nz@hydrogelsmentioning

confidence: 99%

Nanozyme-Engineered Hydrogels for Anti-Inflammation and Skin Regeneration

Kurian,

Singh,

Sagar

et al. 2024

Nano-Micro Lett.

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Inflammatory skin disorders can cause chronic scarring and functional impairments, posing a significant burden on patients and the healthcare system. Conventional therapies, such as corticosteroids and nonsteroidal anti-inflammatory drugs, are limited in efficacy and associated with adverse effects. Recently, nanozyme (NZ)-based hydrogels have shown great promise in addressing these challenges. NZ-based hydrogels possess unique therapeutic abilities by combining the therapeutic benefits of redox nanomaterials with enzymatic activity and the water-retaining capacity of hydrogels. The multifaceted therapeutic effects of these hydrogels include scavenging reactive oxygen species and other inflammatory mediators modulating immune responses toward a pro-regenerative environment and enhancing regenerative potential by triggering cell migration and differentiation. This review highlights the current state of the art in NZ-engineered hydrogels (NZ@hydrogels) for anti-inflammatory and skin regeneration applications. It also discusses the underlying chemo-mechano-biological mechanisms behind their effectiveness. Additionally, the challenges and future directions in this ground, particularly their clinical translation, are addressed. The insights provided in this review can aid in the design and engineering of novel NZ-based hydrogels, offering new possibilities for targeted and personalized skin-care therapies.

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“…This hydrogel promotes wound healing by utilizing the synergistic antibacterial properties of Cu and AgNPs and nanozyme in the hydrogel to remove ROS. 178 Au-AgNCs@ GSH and Cu-Ag nanocluster composite hydrogel are the latest designed dressings. Their common feature is the use of double MNPs to achieve the effect of antibacterial activity enhancement.…”

Section: Clinical Application Of Mnpsmentioning

confidence: 99%

Metal Nanoparticles: Advanced and Promising Technology in Diabetic Wound Therapy

Zheng,

Chen,

Liu

et al. 2024

IJN

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Diabetic wounds pose a significant challenge to public health, primarily due to insufficient blood vessel supply, bacterial infection, excessive oxidative stress, and impaired antioxidant defenses. The aforementioned condition not only places a significant physical burden on patients’ prognosis, but also amplifies the economic strain on the medical system in treating diabetic wounds. Currently, the effectiveness of available treatments for diabetic wounds is limited. However, there is hope in the potential of metal nanoparticles (MNPs) to address these issues. MNPs exhibit excellent anti-inflammatory, antioxidant, antibacterial and pro-angiogenic properties, making them a promising solution for diabetic wounds. In addition, MNPs stimulate the expression of proteins that promote wound healing and serve as drug delivery systems for small-molecule drugs. By combining MNPs with other biomaterials such as hydrogels and chitosan, novel dressings can be developed and revolutionize the treatment of diabetic wounds. The present article provides a comprehensive overview of the research progress on the utilization of MNPs for treating diabetic wounds. Building upon this foundation, we summarize the underlying mechanisms involved in diabetic wound healing and discuss the potential application of MNPs as biomaterials for drug delivery. Furthermore, we provide an extensive analysis and discussion on the clinical implementation of dressings, while also highlighting future prospects for utilizing MNPs in diabetic wound management. In conclusion, MNPs represent a promising strategy for the treatment of diabetic wound healing. Future directions include combining other biological nanomaterials to synthesize new biological dressings or utilizing the other physicochemical properties of MNPs to promote wound healing. Synthetic biomaterials that contain MNPs not only play a role in all stages of diabetic wound healing, but also provide a stable physiological environment for the wound-healing process.

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A multi-enzyme-like activity exhibiting mussel-inspired nanozyme hydrogel for bacteria-infected wound healing

Abstract: Bacterial infection, tissue hypoxia, inflammatory and oxidative stress are several keyproblems in wound healing of chronic infections. Herein, a multi-enzyme-like activitiesmultifunctional hydrogel made up mussel-inspired carbon dots reduced Ag(CDs/AgNPs) and...

Cited by 12 publications

References 60 publications

Ultralow Loading Copper-Intercalated MoO₃ Nanobelts with High Activity against Antibiotic-Resistant Bacteria

Ultralow Loading Copper-Intercalated MoO₃ Nanobelts with High Activity against Antibiotic-Resistant Bacteria

Nanozyme-Engineered Hydrogels for Anti-Inflammation and Skin Regeneration

Metal Nanoparticles: Advanced and Promising Technology in Diabetic Wound Therapy

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A multi-enzyme-like activity exhibiting mussel-inspired nanozyme hydrogel for bacteria-infected wound healing

Abstract: Bacterial infection, tissue hypoxia, inflammatory and oxidative stress are several keyproblems in wound healing of chronic infections. Herein, a multi-enzyme-like activitiesmultifunctional hydrogel made up mussel-inspired carbon dots reduced Ag(CDs/AgNPs) and...

Cited by 12 publications

References 60 publications

Ultralow Loading Copper-Intercalated MoO3 Nanobelts with High Activity against Antibiotic-Resistant Bacteria

Ultralow Loading Copper-Intercalated MoO3 Nanobelts with High Activity against Antibiotic-Resistant Bacteria

Nanozyme-Engineered Hydrogels for Anti-Inflammation and Skin Regeneration

Metal Nanoparticles: Advanced and Promising Technology in Diabetic Wound Therapy

Contact Info

Product

Resources

About

Ultralow Loading Copper-Intercalated MoO₃ Nanobelts with High Activity against Antibiotic-Resistant Bacteria

Ultralow Loading Copper-Intercalated MoO₃ Nanobelts with High Activity against Antibiotic-Resistant Bacteria