Growing environmental concerns have contributed to urban transit alternatives, such as Electric Vehicles (EVS). As a result, the market for EVs is growing each year, which are a solution to mitigate these concerns. Although EVs present several environmental advantages, a massive introduction of them could generate power systems issues. Several works have proposed strategies to mitigate those issues. Since EVs posses batteries with significant capacity, they could provide services to the power grid, such as ancillary services. Thus, this paper presents a methodology where EVs could participate in Secondary frequency response through an EV aggregator. Moreover, EV user's preferences are taken into account to facilitate their participation. The case study of Quito, Ecuador is considered. The results of this methodology show that the EV aggregator has a significant potential for providing electricity market regulation services, especially in view of the use of V2G mode.