Simultaneous high-speed quantitative imaging of mixture fraction and velocity is demonstrated using the fourth-and second-harmonic outputs, respectively, of a burst-mode Nd:YAG laser. A tenfold increase in the record length and 16-fold increase in per-pulse energy are achieved compared with previous measurements of mixture fraction using burst-mode and continuously pulsed diode-pumped solid-state lasers, respectively. The high output energy is used for quantitative, high-speed mixture-fraction imaging with acetone planar laser-induced fluorescence, which also enables simultaneous particle-based velocimetry without interference from particle scattering. A semiquantitative model is used to determine the limitations on fourth-harmonic output energy due to the effects of transient absorption and thermal phase mismatch over a range of repetition rates. Data are presented for mixing within a turbulent jet (Reynolds number of 15,000) and are validated by comparisons with known turbulent mixing laws and previously published data.