“…Take The fragment ion at m/z 779 was produced when the precursor ion lost 42 Da, so it was presumed to be an acetylated compound 76, 78, or 89. However, there were no fragment ions that can be used to distinguish the extra location of the acetylated, comparing literature [12,13] and the different retention times, they were tentatively identified as 2′'-O-acetylsaikosaponin A (Figure 5B The majority of ginseng are protopanaxatriol (PPT) type ginsenosides and protopanaxadiol (PPD) type ginsenosides and contains a small amount of oleanolic acid (OA) type ginsenosides [10,14]. Ginsenosides showed quasi-molecular ion [M-H] − or [M+HCOO] − in negative ion mode, in MS/MS spectra, ginsenosides are prone to breakage of glycosidic bonds, lose sugars (162 Da for Glu, 146 Da for Rha, 176 Da for Glu A), and typical product ions associated with the sapogenins (m/z 475 for PPT, 459 for PPD, and 455 for OA) [10,15,16].…”