A multidisciplinary study on the effects of phloem-limited viruses on the agronomical performance and berry quality of Vitis vinifera cv. Nebbiolo

Giribaldi, Marzia; Purrotti, M.; Pacifico, Davide; Santini, Deborah; Mannini, F.; Caciagli, P.; Rolle, Luca Giorgio Carlo; Cavallarin, Laura; Giuffrida, Maria Gabriella; Marzachı̀, Cristina

doi:10.1016/j.jprot.2011.08.006

Cited by 33 publications

(22 citation statements)

References 75 publications

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“…In particular, differences in berry cohesiveness and resilience were detected in Nebbiolo grapes simultaneously affected by GLRaV-1 and GVA grapevine virus compared to healthy specimens. In fact, proteomics analysis showed that the virus infection mainly influenced the proteins involved in the cell structure metabolism in the pulp with an increase in the cell wall degradation (Giribaldi et al 2011).…”

Section: Evolution Of Texture Parameters During Ripeningmentioning

confidence: 99%

Instrumental Texture Analysis Parameters as Markers of Table-Grape and Winegrape Quality: A Review

Rolle¹,

Siret²,

Segade³

et al. 2011

Am. J. Enol. Vitic.

103

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Texture analysis is a well-established analytical technique in the food industry for evaluating the mechanical and physical characteristics of both raw ingredients and finished products. Instrumental mechanical properties of table grapes, acquired using universal testing machines equipped with specific probes, may be of interest in the viticulture and postharvest sectors to recognize the potential of each variety and help satisfy market requirements. The measured parameters are related to some sensory properties and, therefore, indirectly to consumer acceptability of the product. As texture analysis is a rapid and low-cost analytical technique, it can also be favorably applied in enology as a routine tool for monitoring winegrape quality. Among the different mechanical parameters measurable, berry skin thickness and hardness are indices that reflect anthocyanin extractability and dehydration kinetics with adequate reliability. This review collects the most recent research results from grape texture studies, emphasizes the potentiality and limits of this analytical technique when applied to grape studies, and highlights trends that require further understanding.

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Section: Evolution Of Texture Parameters During Ripeningmentioning

confidence: 99%

Instrumental Texture Analysis Parameters as Markers of Table-Grape and Winegrape Quality: A Review

Rolle¹,

Siret²,

Segade³

et al. 2011

Am. J. Enol. Vitic.

103

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“…The availability of high-throughput analysis methods and a high-quality draft of the grapevine genome sequence (1) have led to the recent application of next-generation sequencing to investigate grape berry development and pathogen infection, although few proteomic studies are available. Upon infection, plants dramatically change at the molecular and biochemical levels as reflected by global transcriptome (2)(3)(4), proteome (5,6), and metabolome (7) system analyses. These studies demonstrate the importance of the complex modulation of plant metabolism and defense responses during pathogen infection.…”

mentioning

confidence: 99%

Modulation of Protein Phosphorylation, N-Glycosylation and Lys-Acetylation in Grape (Vitis vinifera) Mesocarp and Exocarp Owing to Lobesia botrana Infection

Melo‐Braga

Verano‐Braga

León

et al. 2012

Molecular & Cellular Proteomics

112

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Grapevine (Vitis vinifera) is an economically important fruit crop that is subject to many types of insect and pathogen attack. To better elucidate the plant response to Lobesia botrana pathogen infection, we initiated a global comparative proteomic study monitoring steady-state protein expression as well as changes in N-glycosylation, phosphorylation, and Lys-acetylation in control and infected mesocarp and exocarp from V. vinifera cv Italia. A multi-parallel, large-scale proteomic approach employing iTRAQ labeling prior to three peptide enrichment techniques followed by tandem mass spectrometry led to the identification of a total of 3059 proteins, 1135 phosphorylation sites, 323 N-linked glycosylation sites and 138 Lysacetylation sites. Of these, we could identify changes in abundance of 899 proteins. The occupancy of 110 phosphorylation sites, 10 N-glycosylation sites and 20 Lysacetylation sites differentially changed during L. botrana infection. Sequence consensus analysis for phosphorylation sites showed eight significant motifs, two of which containing up-regulated phosphopeptides (X-G-S-X and S-X-X-D) and two containing down-regulated phosphopeptides (R-X-X-S and S-D-X-E) in response to

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“…O amadurecimento irregular dos cachos pode ocasionar aumento da acidez total titulável do mosto da uva (Giribaldi et al, 2011), entretanto, este efeito não foi significativo nas avaliações realizadas. Em geral, o mosto da uva de plantas infectadas tende a apresentar menor pH do que o de plantas sadias (Basso et al, 2014), porém, essa variável também não apresentou diferença significativa nas condições testadas (Tabela 2).…”

Section: Resultsunclassified

“…As plantas infectadas podem apresentar alterações na expressão de genes, redução do potencial fotossintético, aumento da taxa de respiração e redução dos teores de clorofila (Fajardo et al, 2004;Espinoza et al, 2007;Basso et al, 2010b). Especificamente, os efeitos negativos da infecção viral em videiras consistem em redução do teor de sólidos solúveis totais do mosto, maturação irregular e incompleta da uva, redução da qualidade e do rendimento da produção e, em casos mais severos de infecção, podem ocorrer enfraquecimento e atraso das brotações, redução da longevidade e declínio das plantas infectadas (Giribaldi et al, 2011).…”

Section: Introductionunclassified

Desempenho agronômico de videiras com e sem sintomas de viroses, e comparação molecular de isolados virais

Nascimento

Fajardo

Eiras

et al. 2015

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar os efeitos de viroses em videiras sintomáticas e assintomáticas sobre as variáveis agronômicas relacionadas ao vigor das plantas e à qualidade enológica da uva, e comparar os isolados virais obtidos nessas duas condições. Realizaram-se dois experimentos com quatro cultivares. Todas as plantas foram indexadas, por meio da reação em cadeia da polimerase via transcrição reversa (RT-PCR) em tempo real, quanto à provável ocorrência dos seguintes vírus: Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine virus D (GVD), Grapevine leafroll-associated virus (GLRaV-1 ao -4, GLRaV-4 estirpe 5), Grapevine rupestris stem pitting-associated virus (GRSPaV) e Grapevine fleck virus (GFkV). As variáveis avaliadas foram: número de gemas brotadas e não brotadas, número de ramos com ou sem cachos, número total de gemas, número de cachos, massa de cachos frescos, massa total de bagas, massa do engaço, número de bagas por cacho, massa média de baga, sólidos solúveis totais, acidez total titulável, pH, massa de ramos podados ou diâmetros do tronco do porta-enxerto e da copa. Os efeitos negativos foram mais pronunciados nas plantas com sintomas de viroses; no entanto, constatou-se frequentemente que plantas sem sintomas também estavam infectadas. A análise molecular de GRSPaV, GVA e GLRaV-2, isolados de plantas sintomáticas e assintomáticas, resultou em alta percentagem de identidade de nucleotídeos entre isolados homólogos.Termos para indexação: Grapevine, Vitis, complexo do lenho rugoso, enrolamento-da-folha, PCR em tempo real. Agronomic performance of grapevines with and without virus symptoms, and molecular comparison of viral isolatesAbstract -The objective of this work was to evaluate the effect of viroses in symptomatic and asymptomatic grapevines on the agronomic variables related to the plant vigor and the enological quality of grapes, and to compare viral isolates obtained from these two conditions. Two experiments were performed with four cultivars. All plants were indexed by real time, reverse transcription-polymerase chain reaction (RT-PCR) for the probable occurrence of the following viruses: Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine virus D (GVD), Grapevine leafroll-associated virus (GLRaV-1 to -4, GLRaV-4 strain 5), Grapevine rupestris stem pitting-associated virus (GRSPaV), and Grapevine fleck virus (GFkV). The evaluated variables were: number of buds burst and not burst, number of branches with or without bunches, total number of buds, number of bunches, fresh bunch weight, total berry weight, rachis weight, number of berries per bunch, average berry weight, total soluble solids, total titratable acidity, pH, pruned branch weight, or rootstock and scion trunk diameters. The negative effects were more pronounced on the plants with virus symptoms; however, it was observed that plants without symptoms were also often infected. The molecular analysis of GRSPaV, GVA, and GLRaV-2, isolates from symptomatic and asymptomatic plants, resulted in a high perc...

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A multidisciplinary study on the effects of phloem-limited viruses on the agronomical performance and berry quality of Vitis vinifera cv. Nebbiolo

Cited by 33 publications

References 75 publications

Instrumental Texture Analysis Parameters as Markers of Table-Grape and Winegrape Quality: A Review

Instrumental Texture Analysis Parameters as Markers of Table-Grape and Winegrape Quality: A Review

Modulation of Protein Phosphorylation, N-Glycosylation and Lys-Acetylation in Grape (Vitis vinifera) Mesocarp and Exocarp Owing to Lobesia botrana Infection

Desempenho agronômico de videiras com e sem sintomas de viroses, e comparação molecular de isolados virais

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