A multifunctional key to open a new window on the path to natural resources-lessons from a study on chemical composition and biological capability of Paeonia mascula L. from Turkey

Kurt-Celep, İnci; Zengin, Gökhan; Celep, Engin; Dall’Acqua, Stefano; Sut, Stefania; Ferrase, Irene; Ak, Güneş; Uba, Abdullahi Ibrahim; Polat, Rıdvan; Canlı, Deniz; Darendelioğlu, Ekrem; Aumeeruddy, Muhammad Zakariyyah; Shariati, Mohammad Ali; Mahomoodally, Mohamad Fawzi

doi:10.1016/j.fbio.2022.102194

Cited by 10 publications

(28 citation statements)

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“…The methanol extracts exhibited the highest TPC (122.45 ± 0.48 mg GAE/g) and TFC (41.76 ± 0.22 mg RE/g). Also, methanol extracts were the most effective extracts in all antioxidant activity assays (Kurt-Celep et al, 2023). The data obtained from the assays: DPPH, ABTS, CUPRAC,and FRAP as 490.41 ± 0.30,311.70 ± 3.89,543.31 ± 32.27,266.17 ± 8.31 mg TEAC/g, respectively.…”

Section: Discussionmentioning

confidence: 84%

“…In a study, Kurt-Celep et al (2023) investigated the antioxidant activity, enzyme inhibitory, and anticancer properties of aerial parts of Paeonia mascula L. in four different solvents (ethyl acetate, chloroform, methanol, and water) extracts. The methanol extracts exhibited the highest TPC (122.45 ± 0.48 mg GAE/g) and TFC (41.76 ± 0.22 mg RE/g).…”

Section: Discussionmentioning

confidence: 99%

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The phylogenetic relationship based on the ITS, matK and rbcL DNA regions and comparision of chemical contents of twelve Paeonia taxa in Turkey

Cetiz

Turumtay

Burnaz

et al. 2023

Preprint

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Backgroud: Turkey is considered an important gene pool for the distribution of herbaceous Paeonia species. Twelve taxons were recorded in Turkey and all definitions are morphologically and/or anatomically performed. No studies have been conducted based on DNA barcode sequences. In this study, The matK and rbcL genes and ITS region were sequenced to determine phylogenetic relationships of 12 Turkish Paeonia taxa and chemical comparison was performed with root samples. Methods and results: The taxons were collected between May and June 2021 from nine different cities. Leaf materials were used for DNA isolation and ITS, matK and rbcL regions were amplified and sequenced. There was no difference among taxa in terms of rbcL sequences. But the ITS and matK regions distinguished 12 taxa. The genotypes were structured in two groups by ITS and matK data. ITS region distinguished P. peregrina, P. arietina, and P. tenuifolia from other taxa, while matK region distinguished P. arietina and P. witmanniana from other taxa. Both barcode sequences actually showed that the registration of P. mascula subsp. arasicola was faulty and it is 100% similar to P. arietina. Twelve taxa were each found to have characteristic polymorphisms in both the ITS and matK regions that could be used to distinguish the species from each other. The most polymorphism was determined in the ITS region (n=54), while the matK region showed less variability (n=9). We also detected the barcode sequences could successfully discriminate the hybrid polyploid Paoenia species from diploid P. tenuifolia. Except for P. tenuifolia, the other Paeonia species were seen to be hybrid. The methanolic root extracts were examined for total phenolic content, total flavonoid content, and antioxidant activities (by FRAP, ABTS, and DPPH). The results revealed significant variation in extracts, polyphenolic content, and antioxidant properties. In 100 g root, TPC ranged from 204.23 to 2343.89 mg, TFC ranged from 7.73 to 66.16 mg, and FRAP ranged from 523.81 to 4338.62 mg. SC50 values of ABTS ranged from 115.08 to 1115.52 μg/ml and DPPH ranged from 73.83 to 963.59 μg/ml. Conclusion: For the first time, genetic diversity and chemical comparison were performed among Paeonia species along Turkey. It was concluded that 11 of 12 taxa had differences in terms of ITS and matK sequences. It has been shown that barcode sequences must be used for the correct identification of Turkish Paeonia.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

The phylogenetic relationship based on the ITS, matK and rbcL DNA regions and comparision of chemical contents of twelve Paeonia taxa in Turkey

Cetiz

Turumtay

Burnaz

et al. 2023

Preprint

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“…[76][77][78][79] A. caraganae rRNA, a housekeeping gene, was used. [64,70,80] As a result of the analysis, the MMP-2 level increased 1.98 times when untreated HDF cells were compared with H 2 O 2 treated-HDF cells (in Figure 6a). It When EtOH, EtOH/water, and water extracts were applied to HDF cells treated with H 2 O 2 , it was observed that MMP-2 levels decreased by 58.83%, 68.21%, and 87.83%, respectively.…”

Section: Determination Of Intracellular and Mitochondrial Reactive Ox...mentioning

confidence: 94%

“…[64,68,69] Various plants with high antioxidant potential are used for the improvement and rearrangement of ROS-induced impaired molecular signaling pathways. [5,64,70] In studies with Astragalus species, ROSinduced loss of cell cycle in cells is regulated thanks to bioactive components such as various flavonoids, saponins, and alkaloids contained in the plant. [5,71] This information in the literature shows parallelism with the results of Western blot (in Figure 4).…”

Section: Enzyme Inhibitory Effectsmentioning

confidence: 99%

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Unraveling the chemical profile, antioxidant, enzyme inhibitory, cytotoxic potential of different extracts from Astragalus caraganae

Kurt-Celep

Zengin

Uba

et al. 2023

Archiv der Pharmazie

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Six extracts (water, ethanol, ethanol‐water, ethyl acetate, dichloromethane, and n‐hexane) of Astragalus caraganae were studied for their biological activities and bioactive contents. Based on high‐performance liquid chromatography‐mass spectrometry (HPLC‐MS), the ethanol‐water extract yielded the highest total bioactive content (4242.90 µg g−1), followed by the ethanol and water extracts (3721.24 and 3661.37 µg g−1, respectively), while the least total bioactive content was yielded by the hexane extract, followed by the dichloromethane and ethyl acetate extracts (47.44, 274.68, and 688.89 µg g−1, respectively). Rutin, p‐coumaric, chlorogenic, isoquercitrin, and delphindin‐3,5‐diglucoside were among the major components. Unlike the dichloromethane extracts, all the other extracts showed radical scavenging ability in the 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging assay (8.73–52.11 mg Trolox equivalent [TE]/g), while all extracts displayed scavenging property in the 2,2‐azino‐bis(3‐ethylbenzthiazoline‐6‐sulfonic acid) (ABTS) radical scavenging assay (16.18–282.74 mg TE/g). The extracts showed antiacetylcholinesterase (1.27–2.73 mg galantamine equivalent [GALAE]/g), antibutyrylcholinesterase (0.20–5.57 mg GALAE/g) and antityrosinase (9.37–63.56 mg kojic acid equivalent [KAE]/g) effects. The molecular mechanism of the H2O2‐induced oxidative stress pathway was aimed to be elucidated by applying ethanol, ethanol/water and water extracts at 200 µg/mL concentration to human dermal cells (HDFs). A. caraganae in HDF cells had neither a cytotoxic nor genotoxic effect but could have a cytostatic effect in increasing concentrations. The findings have allowed a better insight into the pharmacological potential of the plant, with respect to their chemical entities and bioactive contents, as well as extraction solvents and their polarity.

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Uncovering chemical profiles, biological potentials, and protection effect against ECM destruction in H₂O₂‐treated HDF cells of the extracts of Stachys tundjeliensis

Kurt‐Celep,

Zheleva‐Dimitrova,

Sinan

et al. 2023

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The genus Stachys L., one of the largest genera of the Lamiaceae family, is highly represented in Turkey. This study was conducted to determine the bio‐pharmaceutical potential and phenolic contents of six different extracts from aerial parts of Stachys tundjeliensis. The obtained results showed that the ethanol extract exhibited the highest antioxidant activity in the antioxidant assays. Meanwhile, the ethanol extract displayed strong inhibitory activity against α‐tyrosinase, the dichloromethane extract exhibited potent inhibition against butyrylcholinesterase, and the n‐hexane extract against α‐amylase. Based on ultra‐high performance liquid chromatography coupled to high‐resolution mass spectrometry analysis, more than 90 secondary metabolites, including hydroxybenzoic acid, hydroxycinnamic acid, and their glycosides, acylquinic acids, phenylethanoid glycosides, and various flavonoids were identified or tentatively annotated in the studied S. tundjeliensis extracts. It was observed that the application of S. tundjeliensis eliminated H2O2‐induced oxidative stress. It was determined that protein levels of phospho‐nuclear factor kappa B (NF‐κB), receptor for advanced glycation endproducts, and activator protein‐1, which are activated in the nucleus, decreased, and the synthesis of matrix metalloproteinase (MMP)‐2 and MMP‐9 also decreased to basal levels. Overall, these findings suggest that S. tundjeliensis contains diverse bioactive compounds for the development of nutraceuticals or functional foods with potent biological properties.

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A multifunctional key to open a new window on the path to natural resources-lessons from a study on chemical composition and biological capability of Paeonia mascula L. from Turkey

Cited by 10 publications

References 100 publications

The phylogenetic relationship based on the ITS, matK and rbcL DNA regions and comparision of chemical contents of twelve Paeonia taxa in Turkey

The phylogenetic relationship based on the ITS, matK and rbcL DNA regions and comparision of chemical contents of twelve Paeonia taxa in Turkey

Unraveling the chemical profile, antioxidant, enzyme inhibitory, cytotoxic potential of different extracts from Astragalus caraganae

Uncovering chemical profiles, biological potentials, and protection effect against ECM destruction in H₂O₂‐treated HDF cells of the extracts of Stachys tundjeliensis

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A multifunctional key to open a new window on the path to natural resources-lessons from a study on chemical composition and biological capability of Paeonia mascula L. from Turkey

Cited by 10 publications

References 100 publications

The phylogenetic relationship based on the ITS, matK and rbcL DNA regions and comparision of chemical contents of twelve Paeonia taxa in Turkey

The phylogenetic relationship based on the ITS, matK and rbcL DNA regions and comparision of chemical contents of twelve Paeonia taxa in Turkey

Unraveling the chemical profile, antioxidant, enzyme inhibitory, cytotoxic potential of different extracts from Astragalus caraganae

Uncovering chemical profiles, biological potentials, and protection effect against ECM destruction in H2O2‐treated HDF cells of the extracts of Stachys tundjeliensis

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Product

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Uncovering chemical profiles, biological potentials, and protection effect against ECM destruction in H₂O₂‐treated HDF cells of the extracts of Stachys tundjeliensis